Restriction map for
pFN21AB8952
(Linear) (Six-base) MAPSORT of: KIBB8952.seq Check: 2516 from: 1 to: 981
KIBB8952.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 606 981
Size: 606 375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 981
Size: 833 148
ApaI G_GGCC'C
Cuts at: 0 93 427 981
Size: 93 334 554
Fragments arranged by size:
554 334 93
ApoI r'AATT_y
Cuts at: 0 731 780 918 981
Size: 731 49 138 63
Fragments arranged by size:
731 138 63 49
AvaI C'yCGr_G
Cuts at: 0 74 143 269 399 420 444 981
Size: 74 69 126 130 21 24 537
Fragments arranged by size:
537 130 126 74 69 24 21
AvrII C'CTAG_G
Cuts at: 0 180 981
Size: 180 801
BamHI G'GATC_C
Cuts at: 0 131 981
Size: 131 850
BanII G_rGCy'C
Cuts at: 0 93 388 427 981
Size: 93 295 39 554
Fragments arranged by size:
554 295 93 39
BglI GCCn_nnn'nGGC
Cuts at: 0 439 981
Size: 439 542
Bme1580I G_kGCm'C
Cuts at: 0 93 150 427 981
Size: 93 57 277 554
Fragments arranged by size:
554 277 93 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 391 981
Size: 206 185 590
Fragments arranged by size:
590 206 185
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 96 126 981
Size: 96 30 855
Fragments arranged by size:
855 96 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 257 955 981
Size: 257 698 26
Fragments arranged by size:
698 257 26
BseYI C'CCAG_C
Cuts at: 0 209 331 838 981
Size: 209 122 507 143
Fragments arranged by size:
507 209 143 122
BsiEI CG_ry'CG
Cuts at: 0 364 381 395 431 981
Size: 364 17 14 36 550
Fragments arranged by size:
550 364 36 17 14
BsmBI CGTCTCn'nnnn_
Cuts at: 0 15 981
Size: 15 966
Bsp1286I G_dGCh'C
Cuts at: 0 93 150 388 427 981
Size: 93 57 238 39 554
Fragments arranged by size:
554 238 93 57 39
BsrBI CCG'CTC
Cuts at: 0 33 404 981
Size: 33 371 577
Fragments arranged by size:
577 371 33
BsrFI r'CCGG_y
Cuts at: 0 359 394 463 981
Size: 359 35 69 518
Fragments arranged by size:
518 359 69 35
BstEII G'GTnAC_C
Cuts at: 0 102 981
Size: 102 879
BstYI r'GATC_y
Cuts at: 0 131 981
Size: 131 850
BstZ17I GTA'TAC
Cuts at: 0 607 981
Size: 607 374
Bsu36I CC'TnA_GG
Cuts at: 0 529 981
Size: 529 452
BtgI C'CryG_G
Cuts at: 0 107 251 506 585 981
Size: 107 144 255 79 396
Fragments arranged by size:
396 255 144 107 79
BtsI GCAGTG_nn'
Cuts at: 0 148 981
Size: 148 833
DraI TTT'AAA
Cuts at: 0 545 759 981
Size: 545 214 222
Fragments arranged by size:
545 222 214
EaeI y'GGCC_r
Cuts at: 0 45 361 378 428 516 588 981
Size: 45 316 17 50 88 72 393
Fragments arranged by size:
393 316 88 72 50 45 17
EagI C'GGCC_G
Cuts at: 0 361 378 428 981
Size: 361 17 50 553
Fragments arranged by size:
553 361 50 17
EarI CTCTTCn'nnn_
Cuts at: 0 169 981
Size: 169 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 104 981
Size: 104 877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 391 833 981
Size: 206 185 442 148
Fragments arranged by size:
442 206 185 148
EcoO109I rG'GnC_Cy
Cuts at: 0 89 90 417 981
Size: 89 1 327 564
Fragments arranged by size:
564 327 89 1
EcoRI G'AATT_C
Cuts at: 0 731 981
Size: 731 250
HaeII r_GCGC'y
Cuts at: 0 280 981
Size: 280 701
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 981
Size: 96 32 853
Fragments arranged by size:
853 96 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 981
Size: 947 34
MscI TGG'CCA
Cuts at: 0 590 981
Size: 590 391
MslI CAynn'nnrTG
Cuts at: 0 614 981
Size: 614 367
MspA1I CmG'CkG
Cuts at: 0 24 327 498 508 981
Size: 24 303 171 10 473
Fragments arranged by size:
473 303 171 24 10
NaeI GCC'GGC
Cuts at: 0 465 981
Size: 465 516
NcoI C'CATG_G
Cuts at: 0 585 981
Size: 585 396
NgoMIV G'CCGG_C
Cuts at: 0 463 981
Size: 463 518
PfoI T'CCnGG_A
Cuts at: 0 134 981
Size: 134 847
PpuMI rG'GwC_Cy
Cuts at: 0 417 981
Size: 417 564
PshAI GACnn'nnGTC
Cuts at: 0 7 981
Size: 7 974
PspOMI G'GGCC_C
Cuts at: 0 89 423 981
Size: 89 334 558
Fragments arranged by size:
558 334 89
PstI C_TGCA'G
Cuts at: 0 456 981
Size: 456 525
PvuII CAG'CTG
Cuts at: 0 327 981
Size: 327 654
SacII CC_GC'GG
Cuts at: 0 509 981
Size: 509 472
SfcI C'TryA_G
Cuts at: 0 452 981
Size: 452 529
SmaI CCC'GGG
Cuts at: 0 145 422 981
Size: 145 277 559
Fragments arranged by size:
559 277 145
StyI C'CwwG_G
Cuts at: 0 180 437 585 981
Size: 180 257 148 396
Fragments arranged by size:
396 257 180 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 981
Size: 31 950
TatI w'GTAC_w
Cuts at: 0 948 981
Size: 948 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 598 981
Size: 598 383
XmaI C'CCGG_G
Cuts at: 0 143 420 981
Size: 143 277 561
Fragments arranged by size:
561 277 143
Enzymes that do cut:
AccI AcuI ApaI ApoI AvaI AvrII BamHI BanII
BglI Bme1580I BpmI BsaXI BseRI BseYI BsiEI BsmBI
Bsp1286I BsrBI BsrFI BstEII BstYI BstZ17I Bsu36I BtgI
BtsI DraI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI HaeII Hin4I MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV PfoI PpuMI PshAI PspOMI PstI PvuII
SacII SfcI SmaI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsgI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstXI BtgZI ClaI CspCI
DraIII DrdI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmnI ZraI