(Circular) (Six-base) MAPSORT of: pFN21AB8951.seq Check: 3112 from: 1 to: 5683 pFN21AB8951 5683 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2249 2249 Size: 5683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2547 3026 1937 Size: 610 479 4594 Fragments arranged by size: 4594 610 479 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2714 3746 5130 5398 1632 Size: 1082 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1082 1032 268 AflII C'TTAA_G Cuts at: 820 1017 2903 820 Size: 197 1886 3600 Fragments arranged by size: 3600 1886 197 AflIII A'CryG_T Cuts at: 1203 4582 5559 1203 Size: 3379 977 1327 Fragments arranged by size: 3379 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2069 1184 Size: 885 4798 AlwNI CAG_nnn'CTG Cuts at: 2122 3426 4998 2122 Size: 1304 1572 2807 Fragments arranged by size: 2807 1572 1304 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2078 3008 1870 Size: 73 135 930 4545 Fragments arranged by size: 4545 930 135 73 BamHI G'GATC_C Cuts at: 2400 3013 3380 2400 Size: 613 367 4703 Fragments arranged by size: 4703 613 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 3004 4459 618 Size: 325 217 627 393 824 1455 1842 Fragments arranged by size: 1842 1455 824 627 393 325 217 BanII G_rGCy'C Cuts at: 1391 2285 2499 3002 1391 Size: 894 214 503 4072 Fragments arranged by size: 4072 894 503 214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2605 928 Size: 971 706 4006 Fragments arranged by size: 4006 971 706 BbvCI CC'TCA_GC Cuts at: 2738 2738 Size: 5683 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3874 3908 1903 Size: 34 50 34 1853 34 3678 Fragments arranged by size: 3678 1853 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 2502 1548 Size: 954 4729 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2300 4294 136 Size: 107 122 71 631 605 628 1994 1525 Fragments arranged by size: 1994 1525 631 628 605 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 2079 2185 3744 4900 1790 Size: 289 106 1559 1156 2573 Fragments arranged by size: 2573 1559 1156 289 106 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3414 4372 442 Size: 416 53 83 399 24 1997 958 1753 Fragments arranged by size: 1997 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 4343 1239 Size: 1388 1716 2579 Fragments arranged by size: 2579 1716 1388 Bpu10I CC'TnA_GC Cuts at: 2738 2738 Size: 5683 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 4346 882 Size: 617 575 2272 2219 Fragments arranged by size: 2272 2219 617 575 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3490 3872 4507 275 Size: 53 83 186 936 1957 382 635 1451 Fragments arranged by size: 1957 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2260 2343 4116 4514 4788 4935 5346 2260 Size: 83 1773 398 274 147 411 2597 Fragments arranged by size: 2597 1773 411 398 274 147 83 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5458 Fragments arranged by size: 5458 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2576 2647 1186 Size: 1390 71 4222 Fragments arranged by size: 4222 1390 71 BseYI C'CCAG_C Cuts at: 2801 4886 5382 2801 Size: 2085 496 3102 Fragments arranged by size: 3102 2085 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2922 1163 Size: 573 1186 3924 Fragments arranged by size: 3924 1186 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2058 2285 3744 3829 4900 2058 Size: 227 1459 85 1071 2841 Fragments arranged by size: 2841 1459 1071 227 85 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2079 2185 2285 2499 3002 3744 Size: 399 268 21 106 100 214 503 742 Cuts at: 3744 3829 4900 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 503 399 268 214 106 100 85 21 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2249 3038 844 Size: 1405 789 3489 Fragments arranged by size: 3489 1405 789 BsrBI CCG'CTC Cuts at: 1394 1823 2464 3570 1394 Size: 429 641 1106 3507 Fragments arranged by size: 3507 1106 641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2817 4178 4352 65 Size: 1183 1569 1361 174 1396 Fragments arranged by size: 1569 1396 1361 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2390 4327 4514 1700 Size: 110 580 1937 187 2869 Fragments arranged by size: 2869 1937 580 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 2359 1184 Size: 1175 4508 BstXI CCAn_nnnn'nTGG Cuts at: 2710 2710 Size: 5683 BstYI r'GATC_y Cuts at: 1972 2334 2400 2956 3013 3380 3765 3782 5234 Size: 362 66 556 57 367 385 17 1452 Cuts at: 5234 5320 5332 5678 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 556 385 367 362 346 86 66 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2175 513 Size: 552 1110 4021 Fragments arranged by size: 4021 1110 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2044 487 Size: 683 56 818 4126 Fragments arranged by size: 4126 818 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3993 4013 3199 Size: 794 20 4869 Fragments arranged by size: 4869 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2292 2327 529 Size: 35 1728 35 3885 Fragments arranged by size: 3885 1728 35 35 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2382 2847 Size: 54 1425 50 275 18 342 210 465 Cuts at: 2847 3130 4019 5648 8 Size: 283 889 1629 43 Fragments arranged by size: 1629 1425 889 465 342 283 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2729 2783 2961 3613 1353 Size: 1376 54 178 652 3423 Fragments arranged by size: 3423 1376 652 178 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2283 2283 Size: 5683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2627 2714 3746 4343 5130 5398 1239 Size: 393 995 87 1032 597 787 268 1524 Fragments arranged by size: 1524 1032 995 787 597 393 268 87 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 2123 4830 1678 Size: 445 2707 2531 Fragments arranged by size: 2707 2531 445 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4324 4356 4398 4430 1373 Size: 32 135 32 2752 32 42 32 2626 Fragments arranged by size: 2752 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 2304 3282 2304 Size: 978 4705 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 10 Size: 54 1425 343 552 3309 Fragments arranged by size: 3309 1425 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2531 3641 4000 4159 518 Size: 648 885 480 1110 359 159 2042 Fragments arranged by size: 2042 1110 885 648 480 359 159 MspA1I CmG'CkG Cuts at: 1606 2742 2928 3099 3776 4924 5169 1606 Size: 1136 186 171 677 1148 245 2120 Fragments arranged by size: 2120 1148 1136 677 245 186 171 NaeI GCC'GGC Cuts at: 2392 2392 Size: 5683 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5131 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NgoMIV G'CCGG_C Cuts at: 2390 2390 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 3041 4586 1132 Size: 97 1812 1545 2229 Fragments arranged by size: 2229 1812 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 3035 830 Size: 2205 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2742 2742 Size: 5683 SacI G_AGCT'C Cuts at: 2285 2285 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 2678 3931 5643 2678 Size: 1253 1712 2718 Fragments arranged by size: 2718 1712 1253 SfcI C'TryA_G Cuts at: 826 1045 3031 4166 4847 5038 826 Size: 219 1986 1135 681 191 1471 Fragments arranged by size: 1986 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 2080 3010 1872 Size: 208 930 4545 Fragments arranged by size: 4545 930 208 SmlI C'TyrA_G Cuts at: 820 1017 1943 2903 3787 4688 4950 820 Size: 197 926 960 884 901 262 1553 Fragments arranged by size: 1553 960 926 901 884 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2974 1517 Size: 1457 4226 StyI C'CwwG_G Cuts at: 513 1065 3122 513 Size: 552 2057 3074 Fragments arranged by size: 3074 2057 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 2291 2557 3710 3727 3880 4065 Size: 1255 588 123 266 1153 17 153 185 Cuts at: 4065 4510 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1153 588 445 266 185 153 123 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2538 2676 3929 Size: 274 80 33 51 1443 561 138 1253 Cuts at: 3929 5641 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1253 561 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2485 2485 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 2078 3008 1870 Size: 208 930 4545 Fragments arranged by size: 4545 930 208 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI