(Circular) (Six-base) MAPSORT of: pFN21AB8947.seq Check: 1029 from: 1 to: 5647 pFN21AB8947 5647 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3710 5094 5362 1632 Size: 2078 1384 268 1917 Fragments arranged by size: 2078 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 2806 3390 4962 2806 Size: 584 1572 3491 Fragments arranged by size: 3491 1572 584 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2972 1870 Size: 73 1029 4545 Fragments arranged by size: 4545 1029 73 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2816 2968 4423 618 Size: 325 217 627 1029 152 1455 1842 Fragments arranged by size: 1842 1455 1029 627 325 217 152 BanII G_rGCy'C Cuts at: 1391 2411 2454 2966 1391 Size: 1020 43 512 4072 Fragments arranged by size: 4072 1020 512 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2293 2926 928 Size: 971 394 633 3649 Fragments arranged by size: 3649 971 633 394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2699 2811 4258 Size: 107 122 71 631 605 1027 112 1447 Cuts at: 4258 136 Size: 1525 Fragments arranged by size: 1525 1447 1027 631 605 122 112 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 2516 3064 2516 Size: 548 5099 Bme1580I G_kGCm'C Cuts at: 1790 3708 4864 1790 Size: 1918 1156 2573 Fragments arranged by size: 2573 1918 1156 BmgBI CAC'GTC Cuts at: 1318 2383 1318 Size: 1065 4582 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2327 2378 2861 2882 4307 1239 Size: 962 126 51 483 21 1425 2579 Fragments arranged by size: 2579 1425 962 483 126 51 21 Bpu10I CC'TnA_GC Cuts at: 2228 2474 2228 Size: 246 5401 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2870 2924 3772 4637 4935 2870 Size: 54 848 865 298 3582 Fragments arranged by size: 3582 865 848 298 54 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2354 4310 882 Size: 617 855 1956 2219 Fragments arranged by size: 2219 1956 855 617 BsaAI yAC'GTr Cuts at: 493 1204 2178 2446 3438 493 Size: 711 974 268 992 2702 Fragments arranged by size: 2702 992 974 711 268 BsaBI GATnn'nnATC Cuts at: 5641 5641 Size: 5647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2786 3454 3836 4471 Size: 53 83 186 936 1253 668 382 635 Cuts at: 4471 275 Size: 1451 Fragments arranged by size: 1451 1253 936 668 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2148 2178 2327 2357 1178 Size: 30 165 30 745 30 149 30 4468 Fragments arranged by size: 4468 745 165 149 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2172 2594 2879 1186 Size: 986 422 285 3954 Fragments arranged by size: 3954 986 422 285 BseYI C'CCAG_C Cuts at: 2281 2468 2905 4850 5346 2281 Size: 187 437 1945 496 2582 Fragments arranged by size: 2582 1945 496 437 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 1163 Size: 573 765 4309 Fragments arranged by size: 4309 765 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3708 3793 4864 3708 Size: 85 1071 4491 Fragments arranged by size: 4491 1071 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2411 2454 2966 3708 3793 4864 1391 Size: 399 621 43 512 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 621 512 399 85 43 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 2292 3536 5266 2292 Size: 1244 1730 2673 Fragments arranged by size: 2673 1730 1244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3002 844 Size: 2158 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 2424 2494 1925 Size: 499 70 5078 Fragments arranged by size: 5078 499 70 BssSI C'ACGA_G Cuts at: 2624 3707 4719 2624 Size: 1083 1012 3552 Fragments arranged by size: 3552 1083 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 2704 1184 Size: 1520 4127 BstXI CCAn_nnnn'nTGG Cuts at: 2860 2860 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2130 2844 2977 3344 3729 3746 5198 5284 Size: 158 714 133 367 385 17 1452 86 Cuts at: 5284 5296 5642 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 714 385 367 346 158 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2455 2461 2640 513 Size: 552 939 451 6 179 3520 Fragments arranged by size: 3520 939 552 451 179 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2102 487 Size: 683 56 876 4032 Fragments arranged by size: 4032 876 683 56 BtsI GCAGTG_nn' Cuts at: 3163 3957 3977 3163 Size: 794 20 4833 Fragments arranged by size: 4833 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2172 2236 Size: 54 1425 50 275 18 192 150 64 Cuts at: 2236 2262 2458 2616 2637 2691 2850 2922 3094 Size: 26 196 158 21 54 159 72 172 Cuts at: 3094 3983 5612 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 275 196 192 172 159 158 150 72 64 54 54 50 43 26 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2831 2864 2900 3577 1353 Size: 1478 33 36 677 3423 Fragments arranged by size: 3423 1478 677 36 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2327 2378 2861 2882 3710 4307 Size: 393 569 126 51 483 21 828 597 Cuts at: 4307 5094 5362 1239 Size: 787 268 1524 Fragments arranged by size: 1524 828 787 597 569 483 393 268 126 51 21 EcoNI CCTnn'n_nnAGG Cuts at: 2576 2852 2576 Size: 276 5371 EcoO109I rG'GnC_Cy Cuts at: 2224 2485 2557 2803 2890 2224 Size: 261 72 246 87 4981 Fragments arranged by size: 4981 261 246 87 72 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2362 2911 2362 Size: 549 5098 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2718 2763 4794 1678 Size: 1040 45 2031 2531 Fragments arranged by size: 2531 2031 1040 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2154 2327 2359 2863 Size: 32 135 32 550 32 173 32 504 Cuts at: 2863 2895 4288 4320 4362 4394 1373 Size: 32 1393 32 42 32 2626 Fragments arranged by size: 2626 1393 550 504 173 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2024 2174 2852 10 Size: 54 1425 343 192 150 678 2805 Fragments arranged by size: 2805 1425 678 343 192 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2657 3605 3964 4123 518 Size: 648 1491 948 359 159 2042 Fragments arranged by size: 2042 1491 948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2201 2260 2314 3063 3740 4888 5133 1606 Size: 595 59 54 749 677 1148 245 2120 Fragments arranged by size: 2120 1148 749 677 595 245 59 54 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 3005 4550 1132 Size: 97 1776 1545 2229 Fragments arranged by size: 2229 1776 1545 97 PasI CC'CwG_GG Cuts at: 1348 2566 2647 2728 2764 1348 Size: 1218 81 81 36 4231 Fragments arranged by size: 4231 1218 81 81 36 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5647 PfoI T'CCnGG_A Cuts at: 2304 2838 2304 Size: 534 5113 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PmlI CAC'GTG Cuts at: 2178 2178 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2890 2224 Size: 666 4981 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PstI C_TGCA'G Cuts at: 830 2577 2802 2999 830 Size: 1747 225 197 3478 Fragments arranged by size: 3478 1747 225 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 PvuII CAG'CTG Cuts at: 2314 2314 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2577 2999 2577 Size: 422 5225 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2573 2798 2995 4130 4811 5002 826 Size: 219 1528 225 197 1135 681 191 1471 Fragments arranged by size: 1528 1471 1135 681 225 219 197 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2885 2939 3751 4652 4914 820 Size: 197 926 942 54 812 901 262 1553 Fragments arranged by size: 1553 942 926 901 812 262 197 54 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5647 StyI C'CwwG_G Cuts at: 513 1065 2004 2853 3086 513 Size: 552 939 849 233 3074 Fragments arranged by size: 3074 939 849 552 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2536 3674 3691 3844 4029 4474 325 Size: 1255 956 1138 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1138 956 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3893 5605 96 Size: 274 80 33 51 1443 1916 1712 138 Fragments arranged by size: 1916 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2148 2180 2367 2399 5607 5639 2148 Size: 32 187 32 3208 32 2156 Fragments arranged by size: 3208 2156 187 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2278 2725 2278 Size: 447 5200 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI