(Circular) (Six-base) MAPSORT of: pFN21AB8946.seq Check: 7899 from: 1 to: 5644 pFN21AB8946 5644 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2346 2796 2987 1937 Size: 409 450 191 4594 Fragments arranged by size: 4594 450 409 191 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2173 2773 3707 5091 5359 1632 Size: 541 600 934 1384 268 1917 Fragments arranged by size: 1917 1384 934 600 541 268 AfeI AGC'GCT Cuts at: 2738 2738 Size: 5644 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2040 4543 5520 1203 Size: 837 2503 977 1327 Fragments arranged by size: 2503 1327 977 837 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2765 2797 2765 Size: 32 5612 AlwNI CAG_nnn'CTG Cuts at: 2280 3387 4959 2280 Size: 1107 1572 2965 Fragments arranged by size: 2965 1572 1107 ApaI G_GGCC'C Cuts at: 2511 2511 Size: 5644 ApaLI G'TGCA_C Cuts at: 2270 3701 4857 2270 Size: 1431 1156 3057 Fragments arranged by size: 3057 1431 1156 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AscI GG'CGCG_CC Cuts at: 2559 2559 Size: 5644 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2071 2483 2651 2669 2969 1870 Size: 73 128 412 168 18 300 4545 Fragments arranged by size: 4545 412 300 168 128 73 18 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2223 2290 2450 2646 2965 Size: 325 217 627 436 67 160 196 319 Cuts at: 2965 4420 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 436 325 319 217 196 160 67 BanII G_rGCy'C Cuts at: 1391 2211 2268 2336 2511 2963 1391 Size: 820 57 68 175 452 4072 Fragments arranged by size: 4072 820 452 175 68 57 BbeI G_GCGC'C Cuts at: 2294 2650 2294 Size: 356 5288 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 2670 928 Size: 971 213 558 3902 Fragments arranged by size: 3902 971 558 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2274 2511 3705 4861 1790 Size: 484 237 1194 1156 2573 Fragments arranged by size: 2573 1194 1156 484 237 BmgBI CAC'GTC Cuts at: 1318 2320 1318 Size: 1002 4642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2695 4304 1239 Size: 832 624 1609 2579 Fragments arranged by size: 2579 1609 832 624 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2901 4307 882 Size: 617 1402 1406 2219 Fragments arranged by size: 2219 1406 1402 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2291 2406 2647 3451 Size: 53 83 186 936 758 115 241 804 Cuts at: 3451 3833 4468 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 804 758 635 382 241 186 115 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 2237 2267 2453 Size: 30 165 30 726 30 78 30 186 Cuts at: 2453 2483 1178 Size: 30 4339 Fragments arranged by size: 4339 726 186 165 78 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2137 2276 2461 2642 2930 1186 Size: 837 114 139 185 181 288 3900 Fragments arranged by size: 3900 837 288 185 181 139 114 BseYI C'CCAG_C Cuts at: 2294 4847 5343 2294 Size: 2553 496 2595 Fragments arranged by size: 2595 2553 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2304 3094 3855 4883 1540 Size: 275 186 303 790 761 1028 2301 Fragments arranged by size: 2301 1028 790 761 303 275 186 BsiHKAI G_wGCw'C Cuts at: 2268 2274 2336 3705 3790 4861 2268 Size: 6 62 1369 85 1071 3051 Fragments arranged by size: 3051 1369 1071 85 62 6 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 BsmBI CGTCTCn'nnnn_ Cuts at: 2074 2603 2074 Size: 529 5115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2268 2274 2336 2511 2963 3705 Size: 399 421 57 6 62 175 452 742 Cuts at: 3705 3790 4861 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 452 421 399 175 85 62 57 6 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 2999 844 Size: 1319 836 3489 Fragments arranged by size: 3489 1319 836 BsrBI CCG'CTC Cuts at: 1394 1823 2638 3531 1394 Size: 429 815 893 3507 Fragments arranged by size: 3507 893 815 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2303 2563 2702 4288 4475 1700 Size: 110 493 260 139 1586 187 2869 Fragments arranged by size: 2869 1586 493 260 187 139 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 2559 1925 Size: 634 5010 BssSI C'ACGA_G Cuts at: 2267 3704 4716 2267 Size: 1437 1012 3195 Fragments arranged by size: 3195 1437 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2760 2974 3341 3726 3743 5195 5281 5293 Size: 788 214 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 788 385 367 346 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2230 2256 2391 2520 2532 513 Size: 552 939 226 26 135 129 12 3625 Fragments arranged by size: 3625 939 552 226 135 129 26 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 2245 3160 3954 3974 2245 Size: 915 794 20 3915 Fragments arranged by size: 3915 915 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2325 2337 Size: 54 1425 50 275 18 471 24 12 Cuts at: 2337 2517 2683 2716 3091 3980 5609 8 Size: 180 166 33 375 889 1629 43 Fragments arranged by size: 1629 1425 889 471 375 275 180 166 54 50 43 33 24 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2301 3091 1537 Size: 275 489 790 4090 Fragments arranged by size: 4090 790 489 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2266 2334 2266 Size: 68 5576 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2173 2695 2773 3707 4304 5091 Size: 393 439 102 522 78 934 597 787 Cuts at: 5091 5359 1239 Size: 268 1524 Fragments arranged by size: 1524 934 787 597 522 439 393 268 102 78 EcoNI CCTnn'n_nnAGG Cuts at: 2159 2414 2159 Size: 255 5389 EcoO109I rG'GnC_Cy Cuts at: 2507 2508 2507 Size: 1 5643 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2076 2076 Size: 5644 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2294 2499 2650 2740 2818 4791 1678 Size: 616 205 151 90 78 1973 2531 Fragments arranged by size: 2531 1973 616 205 151 90 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373 Size: 32 135 32 2713 32 42 32 2626 Fragments arranged by size: 2713 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2290 2646 2290 Size: 356 5288 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2175 2191 2499 4758 4942 1305 Size: 870 16 308 2259 184 2007 Fragments arranged by size: 2259 2007 870 308 184 16 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 2718 10 Size: 54 1425 343 495 391 2936 Fragments arranged by size: 2936 1425 495 391 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3602 3961 4120 518 Size: 648 2436 359 159 2042 Fragments arranged by size: 2436 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2232 2258 2534 2586 3060 3737 4885 5130 Size: 626 26 276 52 474 677 1148 245 Cuts at: 5130 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 626 474 276 245 52 26 NaeI GCC'GGC Cuts at: 2305 2565 2704 2305 Size: 260 139 5245 Fragments arranged by size: 5245 260 139 NarI GG'CG_CC Cuts at: 2291 2647 2291 Size: 356 5288 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4153 Fragments arranged by size: 4153 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NgoMIV G'CCGG_C Cuts at: 2303 2563 2702 2303 Size: 260 139 5245 Fragments arranged by size: 5245 260 139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2044 2836 3002 4547 1132 Size: 97 815 792 166 1545 2229 Fragments arranged by size: 2229 1545 815 792 166 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 2040 4543 2040 Size: 2503 3141 PflMI CCAn_nnn'nTGG Cuts at: 1108 2220 1108 Size: 1112 4532 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2507 2507 Size: 5644 PstI C_TGCA'G Cuts at: 830 2289 2996 830 Size: 1459 707 3478 Fragments arranged by size: 3478 1459 707 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SacI G_AGCT'C Cuts at: 2268 2336 2268 Size: 68 5576 SacII CC_GC'GG Cuts at: 2233 2259 2535 2233 Size: 26 276 5342 Fragments arranged by size: 5342 276 26 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2289 2996 2289 Size: 707 4937 ScaI AGT'ACT Cuts at: 2921 3892 5604 2921 Size: 971 1712 2961 Fragments arranged by size: 2961 1712 971 SfcI C'TryA_G Cuts at: 826 1045 2285 2383 2992 4127 4808 4999 826 Size: 219 1240 98 609 1135 681 191 1471 Fragments arranged by size: 1471 1240 1135 681 609 219 191 98 SfoI GGC'GCC Cuts at: 2292 2648 2292 Size: 356 5288 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2485 2971 1872 Size: 613 486 4545 Fragments arranged by size: 4545 613 486 SmlI C'TyrA_G Cuts at: 820 1017 1943 3748 4649 4911 820 Size: 197 926 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 2836 3002 1132 Size: 1704 166 3774 Fragments arranged by size: 3774 1704 166 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2004 3083 513 Size: 552 939 1079 3074 Fragments arranged by size: 3074 1079 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3671 3688 3841 4026 4471 325 Size: 1255 2091 17 153 185 445 1498 Fragments arranged by size: 2091 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2057 2919 3890 Size: 274 80 33 51 1443 80 862 971 Cuts at: 3890 5602 96 Size: 1712 138 Fragments arranged by size: 1712 1443 971 862 274 138 80 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2237 2269 5604 5636 2237 Size: 32 3335 32 2245 Fragments arranged by size: 3335 2245 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2116 1316 Size: 800 4844 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2483 2969 1870 Size: 613 486 4545 Fragments arranged by size: 4545 613 486 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI