Restriction map for
pFN21AB8945
(Circular) (Six-base) MAPSORT of: pFN21AB8945.seq Check: 2396 from: 1 to: 5617
pFN21AB8945 5617 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2217 2728 2217
Size: 511 5106
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2025 2960 1937
Size: 88 935 4594
Fragments arranged by size:
4594 935 88
Acc65I G'GTAC_C
Cuts at: 2599 2938 2599
Size: 339 5278
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3680 5064 5332 1632
Size: 2048 1384 268 1917
Fragments arranged by size:
2048 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2318 820
Size: 197 1301 4119
Fragments arranged by size:
4119 1301 197
AflIII A'CryG_T
Cuts at: 1203 2676 4516 5493 1203
Size: 1473 1840 977 1327
Fragments arranged by size:
1840 1473 1327 977
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 2872 4346 2872
Size: 1474 4143
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2536 1184
Size: 1352 4265
AlwNI CAG_nnn'CTG
Cuts at: 3360 4932 3360
Size: 1572 4045
ApaLI G'TGCA_C
Cuts at: 2840 3674 4830 2840
Size: 834 1156 3627
Fragments arranged by size:
3627 1156 834
ApoI r'AATT_y
Cuts at: 1493 2663 2926 3152 3304 5413 1493
Size: 1170 263 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 263 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2220 2942 1870
Size: 73 141 136 722 4545
Fragments arranged by size:
4545 722 141 136 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2496 2599 2938 4393
Size: 325 217 627 447 262 103 339 1455
Cuts at: 4393 618
Size: 1842
Fragments arranged by size:
1842 1455 627 447 339 325 262 217
103
BanII G_rGCy'C
Cuts at: 1391 2936 1391
Size: 1545 4072
BbeI G_GCGC'C
Cuts at: 2238 2238
Size: 5617
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4646
BbvCI CC'TCA_GC
Cuts at: 2884 2884
Size: 5617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2201 2235 2809 2843 3808 3842 1903
Size: 34 264 34 574 34 965 34 3678
Fragments arranged by size:
3678 965 574 264 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2303 2450 2486
Size: 107 122 71 631 605 631 147 36
Cuts at: 2486 4228 136
Size: 1742 1525
Fragments arranged by size:
1742 1525 631 631 605 147 122 107
71 36
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 2189 2308 2499 2795 2844 3678 4834 1790
Size: 399 119 191 296 49 834 1156 2573
Fragments arranged by size:
2573 1156 834 399 296 191 119 49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4306 442
Size: 416 53 83 399 24 1931 958 1753
Fragments arranged by size:
1931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 2675 4277 1239
Size: 1430 6 1602 2579
Fragments arranged by size:
2579 1602 1430 6
Bpu10I CC'TnA_GC
Cuts at: 2884 2884
Size: 5617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4607 4905 3742
Size: 865 298 4454
Fragments arranged by size:
4454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 2705 5611 2705
Size: 2906 2711
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 2589 3424 3806
Size: 53 83 186 936 702 354 835 382
Cuts at: 3806 4441 275
Size: 635 1451
Fragments arranged by size:
1451 936 835 702 635 382 354 186
83 53
BsaWI w'CCGG_w
Cuts at: 2694 4050 4448 4722 4869 5280 2694
Size: 1356 398 274 147 411 3031
Fragments arranged by size:
3031 1356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 2837 2867 1178
Size: 30 165 30 795 30 609 30 3928
Fragments arranged by size:
3928 795 609 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2206 1186
Size: 1020 4597
BseYI C'CCAG_C
Cuts at: 2427 4820 5316 2427
Size: 2393 496 2728
Fragments arranged by size:
2728 2393 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2506 1163
Size: 573 459 311 4274
Fragments arranged by size:
4274 573 459 311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2445 2481 3067 3828 4856 1540
Size: 275 186 444 36 586 761 1028 2301
Fragments arranged by size:
2301 1028 761 586 444 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2844 3678 3763 4834 2844
Size: 834 85 1071 3627
Fragments arranged by size:
3627 1071 834 85
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
BsmBI CGTCTCn'nnnn_
Cuts at: 2163 2163
Size: 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2308 2499 2795 2844 2936 3678
Size: 399 399 119 191 296 49 92 742
Cuts at: 3678 3763 4834 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 399 296 191 119
92 85 49
BspEI T'CCGG_A
Cuts at: 2694 5280 2694
Size: 2586 3031
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2728 2972 844
Size: 1373 511 244 3489
Fragments arranged by size:
3489 1373 511 244
BsrBI CCG'CTC
Cuts at: 1394 1823 2169 3504 1394
Size: 429 346 1335 3507
Fragments arranged by size:
3507 1335 429 346
BsrDI GCAATG_nn'
Cuts at: 65 1248 4112 4286 65
Size: 1183 2864 174 1396
Fragments arranged by size:
2864 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2237 2878 4261 4448 1700
Size: 110 427 641 1383 187 2869
Fragments arranged by size:
2869 1383 641 427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 2849 1925
Size: 924 4693
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5617
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 2263 1184
Size: 1079 4538
BstXI CCAn_nnnn'nTGG
Cuts at: 2435 2435
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2947 3314 3699 3716 5168 5254 5266 5612
Size: 975 367 385 17 1452 86 12 346
Cuts at: 5612 1972
Size: 1977
Fragments arranged by size:
1977 1452 975 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2363 2445 2481 2552 2574 513
Size: 552 1298 82 36 71 22 3556
Fragments arranged by size:
3556 1298 552 82 71 36 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 2287 2306 2563 3133 3927 3947 2287
Size: 19 257 570 794 20 3957
Fragments arranged by size:
3957 794 570 257 20 19
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 2478 3064
Size: 54 1425 50 275 18 612 36 586
Cuts at: 3064 3953 5582 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 612 586 275 54 50
43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2442 2478 3064 1537
Size: 275 630 36 586 4090
Fragments arranged by size:
4090 630 586 275 36
EarI CTCTTCn'nnn_
Cuts at: 1353 2338 2674 3547 1353
Size: 985 336 873 3423
Fragments arranged by size:
3423 985 873 336
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2561 4204 4578 4724 387
Size: 1064 138 972 1643 374 146 1280
Fragments arranged by size:
1643 1280 1064 972 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2669 2675 3680 4277 5064 5332 1239
Size: 393 1037 6 1005 597 787 268 1524
Fragments arranged by size:
1524 1037 1005 787 597 393 268 6
EcoRI G'AATT_C
Cuts at: 2663 2926 3304 2663
Size: 263 378 4976
Fragments arranged by size:
4976 378 263
FspI TGC'GCA
Cuts at: 2379 4123 2379
Size: 1744 3873
FspAI rTGC'GCAy
Cuts at: 2379 2379
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 2238 4764 1678
Size: 560 2526 2531
Fragments arranged by size:
2531 2526 560
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2791 2823 2852 2858 2884
Size: 32 135 32 1219 32 29 6 26
Cuts at: 2884 2890 4258 4290 4332 4364 1373
Size: 6 1368 32 42 32 2626
Fragments arranged by size:
2626 1368 1219 135 42 32 32 32
32 32 29 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2026 2961 3207 669
Size: 988 281 88 935 246 3079
Fragments arranged by size:
3079 988 935 281 246 88
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KasI G'GCGC_C
Cuts at: 2234 2234
Size: 5617
KpnI G_GTAC'C
Cuts at: 2603 2942 2603
Size: 339 5278
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2697 2709 4731 4915 1305
Size: 1392 12 2022 184 2007
Fragments arranged by size:
2022 2007 1392 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3575 3934 4093 518
Size: 648 2409 359 159 2042
Fragments arranged by size:
2409 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2244 2365 2447 2468 2483 2554 3033 3710
Size: 638 121 82 21 15 71 479 677
Cuts at: 3710 4858 5103 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 638 479 245 121 82
71 21 15
NaeI GCC'GGC
Cuts at: 2239 2880 2239
Size: 641 4976
NarI GG'CG_CC
Cuts at: 2235 2235
Size: 5617
NcoI C'CATG_G
Cuts at: 513 1065 2574 513
Size: 552 1509 3556
Fragments arranged by size:
3556 1509 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NgoMIV G'CCGG_C
Cuts at: 2237 2878 2237
Size: 641 4976
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 2478 3064 2478
Size: 586 5031
NspI r_CATG'y
Cuts at: 1132 1229 2116 2680 2975 4520 1132
Size: 97 887 564 295 1545 2229
Fragments arranged by size:
2229 1545 887 564 295 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5617
PciI A'CATG_T
Cuts at: 2676 4516 2676
Size: 1840 3777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5617
PfoI T'CCnGG_A
Cuts at: 2646 2646
Size: 5617
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2969 830
Size: 2139 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
SacII CC_GC'GG
Cuts at: 2366 2448 2484 2555 2366
Size: 82 36 71 5428
Fragments arranged by size:
5428 82 71 36
SalI G'TCGA_C
Cuts at: 1936 2024 2959 1936
Size: 88 935 4594
Fragments arranged by size:
4594 935 88
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2965 4100 4781 4972 826
Size: 219 1920 1135 681 191 1471
Fragments arranged by size:
1920 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2486 2486
Size: 5617
SfoI GGC'GCC
Cuts at: 2236 2236
Size: 5617
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2086 2944 1872
Size: 214 858 4545
Fragments arranged by size:
4545 858 214
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 3721 4622 4884 820
Size: 197 926 375 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 375 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2116 2975 1132
Size: 984 859 3774
Fragments arranged by size:
3774 984 859
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 2490 2574 3056 513
Size: 552 1425 84 482 3074
Fragments arranged by size:
3074 1425 552 482 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 3661 3814 3999 4444 325
Size: 1255 2064 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 5577 5609 2198
Size: 32 3347 32 2206
Fragments arranged by size:
3347 2206 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2603 2539
Size: 64 5553
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2084 2942 1870
Size: 214 858 4545
Fragments arranged by size:
4545 858 214
XmnI GAAnn'nnTTC
Cuts at: 3746 3746
Size: 5617
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI
SrfI SwaI