Restriction map for
pFN21AB8945
(Linear) (Six-base) MAPSORT of: KIBB8945.seq Check: 9055 from: 1 to: 912
KIBB8945.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 212 723 912
Size: 212 511 189
Fragments arranged by size:
511 212 189
AccI GT'mk_AC
Cuts at: 0 20 912
Size: 20 892
Acc65I G'GTAC_C
Cuts at: 0 594 912
Size: 594 318
AflII C'TTAA_G
Cuts at: 0 313 912
Size: 313 599
AflIII A'CryG_T
Cuts at: 0 671 912
Size: 671 241
AhdI GACnn_n'nnGTC
Cuts at: 0 867 912
Size: 867 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 531 912
Size: 531 381
ApaLI G'TGCA_C
Cuts at: 0 835 912
Size: 835 77
ApoI r'AATT_y
Cuts at: 0 658 912
Size: 658 254
AvaI C'yCGr_G
Cuts at: 0 79 215 912
Size: 79 136 697
Fragments arranged by size:
697 136 79
BanI G'GyrC_C
Cuts at: 0 229 491 594 912
Size: 229 262 103 318
Fragments arranged by size:
318 262 229 103
BbeI G_GCGC'C
Cuts at: 0 233 912
Size: 233 679
BbvCI CC'TCA_GC
Cuts at: 0 879 912
Size: 879 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 230 804 838 912
Size: 196 34 574 34 74
Fragments arranged by size:
574 196 74 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 298 445 481 912
Size: 298 147 36 431
Fragments arranged by size:
431 298 147 36
Bme1580I G_kGCm'C
Cuts at: 0 184 303 494 790 839 912
Size: 184 119 191 296 49 73
Fragments arranged by size:
296 191 184 119 73 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 670 912
Size: 664 6 242
Fragments arranged by size:
664 242 6
Bpu10I CC'TnA_GC
Cuts at: 0 879 912
Size: 879 33
BsaBI GATnn'nnATC
Cuts at: 0 700 912
Size: 700 212
BsaHI Gr'CG_yC
Cuts at: 0 230 584 912
Size: 230 354 328
Fragments arranged by size:
354 328 230
BsaWI w'CCGG_w
Cuts at: 0 689 912
Size: 689 223
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 832 862 912
Size: 193 30 609 30 50
Fragments arranged by size:
609 193 50 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 201 912
Size: 201 711
BseYI C'CCAG_C
Cuts at: 0 422 912
Size: 422 490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 501 912
Size: 190 311 411
Fragments arranged by size:
411 311 190
BsiEI CG_ry'CG
Cuts at: 0 440 476 912
Size: 440 36 436
Fragments arranged by size:
440 436 36
BsiHKAI G_wGCw'C
Cuts at: 0 839 912
Size: 839 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 158 912
Size: 158 754
Bsp1286I G_dGCh'C
Cuts at: 0 184 303 494 790 839 912
Size: 184 119 191 296 49 73
Fragments arranged by size:
296 191 184 119 73 49
BspEI T'CCGG_A
Cuts at: 0 689 912
Size: 689 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 723 912
Size: 212 511 189
Fragments arranged by size:
511 212 189
BsrBI CCG'CTC
Cuts at: 0 164 912
Size: 164 748
BsrFI r'CCGG_y
Cuts at: 0 232 873 912
Size: 232 641 39
Fragments arranged by size:
641 232 39
BssHII G'CGCG_C
Cuts at: 0 844 912
Size: 844 68
BstEII G'GTnAC_C
Cuts at: 0 258 912
Size: 258 654
BstXI CCAn_nnnn'nTGG
Cuts at: 0 430 912
Size: 430 482
BtgI C'CryG_G
Cuts at: 0 358 440 476 547 569 912
Size: 358 82 36 71 22 343
Fragments arranged by size:
358 343 82 71 36 22
BtsI GCAGTG_nn'
Cuts at: 0 282 301 558 912
Size: 282 19 257 354
Fragments arranged by size:
354 282 257 19
EaeI y'GGCC_r
Cuts at: 0 437 473 912
Size: 437 36 439
Fragments arranged by size:
439 437 36
EagI C'GGCC_G
Cuts at: 0 437 473 912
Size: 437 36 439
Fragments arranged by size:
439 437 36
EarI CTCTTCn'nnn_
Cuts at: 0 333 669 912
Size: 333 336 243
Fragments arranged by size:
336 333 243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 556 912
Size: 556 356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 670 912
Size: 664 6 242
Fragments arranged by size:
664 242 6
EcoRI G'AATT_C
Cuts at: 0 658 912
Size: 658 254
FspI TGC'GCA
Cuts at: 0 374 912
Size: 374 538
FspAI rTGC'GCAy
Cuts at: 0 374 912
Size: 374 538
HaeII r_GCGC'y
Cuts at: 0 233 912
Size: 233 679
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 786 818 847 853 879 885 912
Size: 786 32 29 6 26 6 27
Fragments arranged by size:
786 32 29 27 26 6 6
HincII GTy'rAC
Cuts at: 0 21 912
Size: 21 891
KasI G'GCGC_C
Cuts at: 0 229 912
Size: 229 683
KpnI G_GTAC'C
Cuts at: 0 598 912
Size: 598 314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 704 912
Size: 692 12 208
Fragments arranged by size:
692 208 12
MspA1I CmG'CkG
Cuts at: 0 239 360 442 463 478 549 912
Size: 239 121 82 21 15 71 363
Fragments arranged by size:
363 239 121 82 71 21 15
NaeI GCC'GGC
Cuts at: 0 234 875 912
Size: 234 641 37
Fragments arranged by size:
641 234 37
NarI GG'CG_CC
Cuts at: 0 230 912
Size: 230 682
NcoI C'CATG_G
Cuts at: 0 569 912
Size: 569 343
NgoMIV G'CCGG_C
Cuts at: 0 232 873 912
Size: 232 641 39
Fragments arranged by size:
641 232 39
NotI GC'GGCC_GC
Cuts at: 0 473 912
Size: 473 439
NspI r_CATG'y
Cuts at: 0 111 675 912
Size: 111 564 237
Fragments arranged by size:
564 237 111
PciI A'CATG_T
Cuts at: 0 671 912
Size: 671 241
PfoI T'CCnGG_A
Cuts at: 0 641 912
Size: 641 271
SacII CC_GC'GG
Cuts at: 0 361 443 479 550 912
Size: 361 82 36 71 362
Fragments arranged by size:
362 361 82 71 36
SalI G'TCGA_C
Cuts at: 0 19 912
Size: 19 893
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 481 912
Size: 481 431
SfoI GGC'GCC
Cuts at: 0 231 912
Size: 231 681
SmaI CCC'GGG
Cuts at: 0 81 912
Size: 81 831
SmlI C'TyrA_G
Cuts at: 0 313 912
Size: 313 599
SphI G_CATG'C
Cuts at: 0 111 912
Size: 111 801
StyI C'CwwG_G
Cuts at: 0 485 569 912
Size: 485 84 343
Fragments arranged by size:
485 343 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 912
Size: 193 32 687
Fragments arranged by size:
687 193 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 598 912
Size: 534 64 314
Fragments arranged by size:
534 314 64
XmaI C'CCGG_G
Cuts at: 0 79 912
Size: 79 833
Enzymes that do cut:
AarI AccI Acc65I AflII AflIII AhdI AlfI ApaLI
ApoI AvaI BanI BbeI BbvCI BcgI BglI Bme1580I
BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BstEII BstXI BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I
HincII KasI KpnI MmeI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PfoI SacII SalI SfiI
SfoI SmaI SmlI SphI StyI TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AgeI AleI AloI AlwNI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BanII
BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsiWI BsmI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI HindIII HpaI MfeI MluI
MscI MslI NdeI NheI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XhoI XmnI ZraI