(Circular) (Six-base) MAPSORT of: pFN21AB8935.seq Check: 6059 from: 1 to: 5557 pFN21AB8935 5557 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3620 5004 5272 1632 Size: 1988 1384 268 1917 Fragments arranged by size: 1988 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 2738 4456 5433 1203 Size: 1535 1718 977 1327 Fragments arranged by size: 1718 1535 1327 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 3300 4872 3300 Size: 1572 3985 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2566 2866 3092 3244 5353 1493 Size: 1073 300 226 152 2109 1697 Fragments arranged by size: 2109 1697 1073 300 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2319 2346 2882 1870 Size: 73 376 27 536 4545 Fragments arranged by size: 4545 536 376 73 27 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2316 2349 2316 Size: 33 5524 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2350 2878 4333 618 Size: 325 217 627 563 528 1455 1842 Fragments arranged by size: 1842 1455 627 563 528 325 217 BanII G_rGCy'C Cuts at: 1391 2876 1391 Size: 1485 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4586 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2223 2257 3748 3782 1903 Size: 34 286 34 1491 34 3678 Fragments arranged by size: 3678 1491 286 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4168 136 Size: 107 122 71 631 605 2496 1525 Fragments arranged by size: 2496 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 2353 3618 4774 1790 Size: 563 1265 1156 2573 Fragments arranged by size: 2573 1265 1156 563 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3288 4246 442 Size: 416 53 83 399 24 1871 958 1753 Fragments arranged by size: 1871 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4217 1239 Size: 2978 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3682 4547 4845 3682 Size: 865 298 4394 Fragments arranged by size: 4394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4220 882 Size: 617 2721 2219 Fragments arranged by size: 2721 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3348 493 Size: 711 2144 2702 Fragments arranged by size: 2702 2144 711 BsaBI GATnn'nnATC Cuts at: 5551 5551 Size: 5557 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3364 3746 4381 275 Size: 53 83 186 936 1831 382 635 1451 Fragments arranged by size: 1831 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3990 4388 4662 4809 5220 3990 Size: 398 274 147 411 4327 Fragments arranged by size: 4327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5332 Fragments arranged by size: 5332 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5557 BseYI C'CCAG_C Cuts at: 4760 5256 4760 Size: 496 5061 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 1163 Size: 573 657 4327 Fragments arranged by size: 4327 657 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3007 3768 4796 1540 Size: 275 186 1006 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3618 3703 4774 3618 Size: 85 1071 4401 Fragments arranged by size: 4401 1071 85 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2353 2876 3618 3703 4774 1391 Size: 399 563 523 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 563 523 399 85 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2239 2912 844 Size: 1395 673 3489 Fragments arranged by size: 3489 1395 673 BsrBI CCG'CTC Cuts at: 1394 1823 3444 1394 Size: 429 1621 3507 Fragments arranged by size: 3507 1621 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2293 2427 4052 4226 65 Size: 1183 1045 134 1625 174 1396 Fragments arranged by size: 1625 1396 1183 1045 174 134 BsrFI r'CCGG_y Cuts at: 1700 1810 4201 4388 1700 Size: 110 2391 187 2869 Fragments arranged by size: 2869 2391 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 3617 4629 3617 Size: 1012 4545 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 2410 4361 2410 Size: 1951 3606 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2038 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2459 2887 3254 3639 3656 5108 5194 5206 Size: 487 428 367 385 17 1452 86 12 Cuts at: 5206 5552 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 487 428 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2256 2256 Size: 5557 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4066 Fragments arranged by size: 4066 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2226 487 Size: 683 56 1000 3818 Fragments arranged by size: 3818 1000 683 56 BtsI GCAGTG_nn' Cuts at: 3073 3867 3887 3073 Size: 794 20 4743 Fragments arranged by size: 4743 794 20 ClaI AT'CG_AT Cuts at: 1554 2842 1554 Size: 1288 4269 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2695 2730 529 Size: 35 2131 35 3356 Fragments arranged by size: 3356 2131 35 35 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2018 4564 809 Size: 507 702 2546 1802 Fragments arranged by size: 2546 1802 702 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2703 3004 3893 Size: 54 1425 50 275 18 873 301 889 Cuts at: 3893 5522 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 873 301 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3004 1537 Size: 275 1192 4090 Fragments arranged by size: 4090 1192 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2412 3487 1353 Size: 1059 1075 3423 Fragments arranged by size: 3423 1075 1059 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4144 4518 4664 387 Size: 1064 138 2555 374 146 1280 Fragments arranged by size: 2555 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3620 4217 5004 5272 1239 Size: 393 1988 597 787 268 1524 Fragments arranged by size: 1988 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2718 2718 Size: 5557 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 EcoRV GAT'ATC Cuts at: 2538 2538 Size: 5557 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 4704 1678 Size: 3026 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2302 2334 2527 Size: 32 135 32 605 32 93 32 193 Cuts at: 2527 2559 4198 4230 4272 4304 1373 Size: 32 1639 32 42 32 2626 Fragments arranged by size: 2626 1639 605 193 135 93 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 2433 3156 2433 Size: 723 4834 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4671 4855 1305 Size: 3366 184 2007 Fragments arranged by size: 3366 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2705 10 Size: 54 1425 343 873 2862 Fragments arranged by size: 2862 1425 873 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3515 3874 4033 518 Size: 648 2349 359 159 2042 Fragments arranged by size: 2349 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2283 2973 3650 4798 5043 1606 Size: 677 690 677 1148 245 2120 Fragments arranged by size: 2120 1148 690 677 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4066 Fragments arranged by size: 4066 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2742 2915 4460 1132 Size: 97 1513 173 1545 2229 Fragments arranged by size: 2229 1545 1513 173 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5557 PciI A'CATG_T Cuts at: 2738 4456 2738 Size: 1718 3839 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5557 PfoI T'CCnGG_A Cuts at: 2168 2168 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2718 2718 Size: 5557 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PstI C_TGCA'G Cuts at: 830 2079 2404 2909 830 Size: 1249 325 505 3478 Fragments arranged by size: 3478 1249 505 325 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SanDI GG'GwC_CC Cuts at: 2718 2718 Size: 5557 SapI GCTCTTCn'nnn_ Cuts at: 2412 2412 Size: 5557 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 2275 3805 5517 2275 Size: 1530 1712 2315 Fragments arranged by size: 2315 1712 1530 SfcI C'TryA_G Cuts at: 826 1045 2075 2400 2905 4040 4721 4912 826 Size: 219 1030 325 505 1135 681 191 1471 Fragments arranged by size: 1471 1135 1030 681 505 325 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2884 1872 Size: 1012 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2319 3661 4562 4824 820 Size: 197 926 376 1342 901 262 1553 Fragments arranged by size: 1553 1342 926 901 376 262 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 2053 2596 3481 5 Size: 47 2001 543 885 2081 Fragments arranged by size: 2081 2001 885 543 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2996 513 Size: 552 939 992 3074 Fragments arranged by size: 3074 992 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3584 3601 3754 3939 4384 325 Size: 1255 2004 17 153 185 445 1498 Fragments arranged by size: 2004 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2123 2273 3803 Size: 274 80 33 51 1443 146 150 1530 Cuts at: 3803 5515 96 Size: 1712 138 Fragments arranged by size: 1712 1530 1443 274 150 146 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2839 2871 5517 5549 2839 Size: 32 2646 32 2847 Fragments arranged by size: 2847 2646 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 2319 1943 Size: 376 5181 XmaI C'CCGG_G Cuts at: 1870 2882 1870 Size: 1012 4545 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI