(Circular) (Six-base) MAPSORT of: pFN21AB8934.seq Check: 9735 from: 1 to: 5557 pFN21AB8934 5557 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2225 3367 278 Size: 53 83 186 936 689 1142 2468 Fragments arranged by size: 2468 1142 936 689 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3620 5004 5272 1632 Size: 1988 1384 268 1917 Fragments arranged by size: 1988 1917 1384 268 AfeI AGC'GCT Cuts at: 2848 2848 Size: 5557 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 2439 4456 5433 1203 Size: 1236 2017 977 1327 Fragments arranged by size: 2017 1327 1236 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 2458 3300 4872 2458 Size: 842 1572 3143 Fragments arranged by size: 3143 1572 842 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2866 3092 3244 5353 1493 Size: 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2882 1870 Size: 73 939 4545 Fragments arranged by size: 4545 939 73 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2119 2209 2878 4333 618 Size: 325 217 627 332 90 669 1455 1842 Fragments arranged by size: 1842 1455 669 627 332 325 217 90 BanII G_rGCy'C Cuts at: 1391 2013 2291 2524 2876 1391 Size: 622 278 233 352 4072 Fragments arranged by size: 4072 622 352 278 233 BbeI G_GCGC'C Cuts at: 2123 2123 Size: 5557 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4586 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 4168 136 Size: 107 122 71 631 605 649 1847 1525 Fragments arranged by size: 1847 1525 649 631 605 122 107 71 BglII A'GATC_T Cuts at: 2748 5552 2748 Size: 2804 2753 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 2214 2308 3618 4774 1790 Size: 424 94 1310 1156 2573 Fragments arranged by size: 2573 1310 1156 424 94 BmgBI CAC'GTC Cuts at: 1318 2440 1318 Size: 1122 4435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3288 4246 442 Size: 416 53 83 399 24 1871 958 1753 Fragments arranged by size: 1871 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2490 2593 2717 4217 1239 Size: 1251 103 124 1500 2579 Fragments arranged by size: 2579 1500 1251 124 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3682 4547 4845 3682 Size: 865 298 4394 Fragments arranged by size: 4394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4220 882 Size: 617 2721 2219 Fragments arranged by size: 2721 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3348 493 Size: 711 2144 2702 Fragments arranged by size: 2702 2144 711 BsaBI GATnn'nnATC Cuts at: 2786 5551 2786 Size: 2765 2792 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2120 2222 2631 3364 Size: 53 83 186 936 587 102 409 733 Cuts at: 3364 3746 4381 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 733 635 587 409 382 186 102 83 53 BsaWI w'CCGG_w Cuts at: 3990 4388 4662 4809 5220 3990 Size: 398 274 147 411 4327 Fragments arranged by size: 4327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2690 2720 1178 Size: 30 165 30 1287 30 4015 Fragments arranged by size: 4015 1287 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5557 BseYI C'CCAG_C Cuts at: 2059 2113 2227 2366 4760 5256 2059 Size: 54 114 139 2394 496 2360 Fragments arranged by size: 2394 2360 496 139 114 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2471 1163 Size: 573 735 4249 Fragments arranged by size: 4249 735 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2352 2653 3007 3768 4796 1540 Size: 275 186 351 301 354 761 1028 2301 Fragments arranged by size: 2301 1028 761 354 351 301 275 186 BsiHKAI G_wGCw'C Cuts at: 2291 2467 3618 3703 4774 2291 Size: 176 1151 85 1071 3074 Fragments arranged by size: 3074 1151 1071 176 85 BsmI GAATG_Cn' Cuts at: 2664 3068 3161 2664 Size: 404 93 5060 Fragments arranged by size: 5060 404 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2214 2291 2308 2467 2524 2876 Size: 399 223 201 77 17 159 57 352 Cuts at: 2876 3618 3703 4774 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 223 201 159 85 77 57 17 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2912 844 Size: 2068 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2066 2701 3444 1394 Size: 429 243 635 743 3507 Fragments arranged by size: 3507 743 635 429 243 BsrDI GCAATG_nn' Cuts at: 65 1248 2195 4052 4226 65 Size: 1183 947 1857 174 1396 Fragments arranged by size: 1857 1396 1183 947 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4201 4388 1700 Size: 110 2391 187 2869 Fragments arranged by size: 2869 2391 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 3617 4629 3617 Size: 1012 4545 BstAPI GCAn_nnn'nTGC Cuts at: 794 2600 794 Size: 1806 3751 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2434 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2158 2748 2887 3254 3639 3656 5108 5194 Size: 186 590 139 367 385 17 1452 86 Cuts at: 5194 5206 5552 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 590 385 367 346 186 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2334 2346 513 Size: 552 939 330 12 3724 Fragments arranged by size: 3724 939 552 330 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 3073 3867 3887 3073 Size: 794 20 4743 Fragments arranged by size: 4743 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2365 2400 529 Size: 35 1801 35 3686 Fragments arranged by size: 3686 1801 35 35 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2349 2424 Size: 54 1425 50 275 18 492 27 75 Cuts at: 2424 2650 3004 3893 5522 8 Size: 226 354 889 1629 43 Fragments arranged by size: 1629 1425 889 492 354 275 226 75 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2349 2650 3004 1537 Size: 275 537 301 354 4090 Fragments arranged by size: 4090 537 354 301 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 3487 1353 Size: 999 1135 3423 Fragments arranged by size: 3423 1135 999 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2092 2189 4144 4518 4664 387 Size: 1064 138 503 97 1955 374 146 1280 Fragments arranged by size: 1955 1280 1064 503 374 146 138 97 EcoICRI GAG'CTC Cuts at: 2289 2289 Size: 5557 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2490 2593 2717 3620 4217 5004 5272 Size: 393 858 103 124 903 597 787 268 Cuts at: 5272 1239 Size: 1524 Fragments arranged by size: 1524 903 858 787 597 393 268 124 103 EcoO109I rG'GnC_Cy Cuts at: 2579 2579 Size: 5557 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 EcoRV GAT'ATC Cuts at: 2047 2402 2047 Size: 355 5202 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 2123 2779 2850 4704 1678 Size: 445 656 71 1854 2531 Fragments arranged by size: 2531 1854 656 445 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373 Size: 32 135 32 2626 32 42 32 2626 Fragments arranged by size: 2626 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2143 2901 3147 669 Size: 988 281 205 758 246 3079 Fragments arranged by size: 3079 988 758 281 246 205 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KasI G'GCGC_C Cuts at: 2119 2119 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4671 4855 1305 Size: 3366 184 2007 Fragments arranged by size: 3366 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 10 Size: 54 1425 343 594 3141 Fragments arranged by size: 3141 1425 594 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3515 3874 4033 518 Size: 648 2349 359 159 2042 Fragments arranged by size: 2349 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2117 2336 2613 2973 3650 4798 5043 1606 Size: 511 219 277 360 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 511 360 277 245 219 NarI GG'CG_CC Cuts at: 2120 2120 Size: 5557 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4066 Fragments arranged by size: 4066 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 2650 3004 2650 Size: 354 5203 NspI r_CATG'y Cuts at: 1132 1229 2915 4460 1132 Size: 97 1686 1545 2229 Fragments arranged by size: 2229 1686 1545 97 PasI CC'CwG_GG Cuts at: 1348 2204 2215 1348 Size: 856 11 4690 Fragments arranged by size: 4690 856 11 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 2204 2422 1108 Size: 1096 218 4243 Fragments arranged by size: 4243 1096 218 PfoI T'CCnGG_A Cuts at: 2282 2395 2404 2467 2282 Size: 113 9 63 5372 Fragments arranged by size: 5372 113 63 9 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2579 2579 Size: 5557 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PstI C_TGCA'G Cuts at: 830 2909 830 Size: 2079 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 SacI G_AGCT'C Cuts at: 2291 2291 Size: 5557 SacII CC_GC'GG Cuts at: 2337 2337 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SexAI A'CCwGG_T Cuts at: 2575 2575 Size: 5557 SfcI C'TryA_G Cuts at: 826 1045 2905 4040 4721 4912 826 Size: 219 1860 1135 681 191 1471 Fragments arranged by size: 1860 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2121 2121 Size: 5557 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2884 1872 Size: 1012 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3661 4562 4824 820 Size: 197 926 1718 901 262 1553 Fragments arranged by size: 1718 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2040 2154 2730 2832 2996 Size: 552 939 24 12 114 576 102 164 Cuts at: 2996 513 Size: 3074 Fragments arranged by size: 3074 939 576 552 164 114 102 24 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2127 2211 2450 3584 3601 3754 3939 Size: 1255 547 84 239 1134 17 153 185 Cuts at: 3939 4384 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1134 547 445 239 185 153 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3803 5515 96 Size: 274 80 33 51 1443 1826 1712 138 Fragments arranged by size: 1826 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5517 5549 5517 Size: 32 5525 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2201 2583 2201 Size: 382 5175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5557 XmaI C'CCGG_G Cuts at: 1870 2882 1870 Size: 1012 4545 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2223 3365 276 Size: 53 83 186 936 689 1142 2468 Fragments arranged by size: 2468 1142 936 689 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI