(Circular) (Six-base) MAPSORT of: pFN21AB8931.seq Check: 3095 from: 1 to: 5551 pFN21AB8931 5551 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3361 278 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2894 1937 Size: 957 4594 Acc65I G'GTAC_C Cuts at: 2625 2872 2625 Size: 247 5304 AclI AA'CG_TT Cuts at: 1563 3678 4051 1563 Size: 2115 373 3063 Fragments arranged by size: 3063 2115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 3614 4998 5266 1632 Size: 713 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 713 268 AfeI AGC'GCT Cuts at: 2373 2373 Size: 5551 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 2151 4450 5427 1203 Size: 948 2299 977 1327 Fragments arranged by size: 2299 1327 977 948 AgeI A'CCGG_T Cuts at: 4382 4382 Size: 5551 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5551 AlwNI CAG_nnn'CTG Cuts at: 2500 3294 4866 2500 Size: 794 1572 3185 Fragments arranged by size: 3185 1572 794 ApaI G_GGCC'C Cuts at: 2364 2364 Size: 5551 ApaLI G'TGCA_C Cuts at: 3608 4764 3608 Size: 1156 4395 ApoI r'AATT_y Cuts at: 1493 2860 3086 3238 5347 1493 Size: 1367 226 152 2109 1697 Fragments arranged by size: 2109 1697 1367 226 152 AscI GG'CGCG_CC Cuts at: 2616 2616 Size: 5551 AseI AT'TA_AT Cuts at: 160 1032 4105 160 Size: 872 3073 1606 Fragments arranged by size: 3073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2114 2350 2363 2876 1870 Size: 73 87 84 236 13 513 4545 Fragments arranged by size: 4545 513 236 87 84 73 13 BamHI G'GATC_C Cuts at: 2881 3248 2881 Size: 367 5184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2546 2625 2872 4327 618 Size: 325 217 627 759 79 247 1455 1842 Fragments arranged by size: 1842 1455 759 627 325 247 217 79 BanII G_rGCy'C Cuts at: 1391 2364 2604 2870 1391 Size: 973 240 266 4072 Fragments arranged by size: 4072 973 266 240 BbeI G_GCGC'C Cuts at: 2550 2550 Size: 5551 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3742 3776 1903 Size: 34 1805 34 3678 Fragments arranged by size: 3678 1805 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3443 4653 3443 Size: 1210 4341 BclI T'GATC_A Cuts at: 1548 1548 Size: 5551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2395 4162 136 Size: 107 122 71 631 605 723 1767 1525 Fragments arranged by size: 1767 1525 723 631 605 122 107 71 BglII A'GATC_T Cuts at: 2781 5546 2781 Size: 2765 2786 BlpI GC'TnA_GC Cuts at: 2968 2968 Size: 5551 Bme1580I G_kGCm'C Cuts at: 1790 2364 3612 4768 1790 Size: 574 1248 1156 2573 Fragments arranged by size: 2573 1248 1156 574 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5551 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2213 3282 4240 Size: 416 53 83 399 24 796 1069 958 Cuts at: 4240 442 Size: 1753 Fragments arranged by size: 1753 1069 958 796 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2497 2711 4211 1239 Size: 1166 92 214 1500 2579 Fragments arranged by size: 2579 1500 1166 214 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3676 4541 4839 3676 Size: 865 298 4388 Fragments arranged by size: 4388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4214 882 Size: 617 2715 2219 Fragments arranged by size: 2715 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2250 3342 493 Size: 711 1046 1092 2702 Fragments arranged by size: 2702 1092 1046 711 BsaBI GATnn'nnATC Cuts at: 5545 5545 Size: 5551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2547 3358 3740 4375 Size: 53 83 186 936 1014 811 382 635 Cuts at: 4375 275 Size: 1451 Fragments arranged by size: 1451 1014 936 811 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2796 3984 4382 4656 4803 5214 2796 Size: 1188 398 274 147 411 3133 Fragments arranged by size: 3133 1188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2141 2171 2471 2501 2684 Size: 30 165 30 738 30 300 30 183 Cuts at: 2684 2714 1178 Size: 30 4015 Fragments arranged by size: 4015 738 300 183 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 1186 Size: 1162 4389 BseYI C'CCAG_C Cuts at: 4754 5250 4754 Size: 496 5055 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4978 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2245 3001 3762 4790 1540 Size: 275 186 244 756 761 1028 2301 Fragments arranged by size: 2301 1028 761 756 275 244 186 BsiHKAI G_wGCw'C Cuts at: 2159 3612 3697 4768 2159 Size: 1453 85 1071 2942 Fragments arranged by size: 2942 1453 1071 85 BsmI GAATG_Cn' Cuts at: 3062 3155 3062 Size: 93 5458 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2364 2604 2870 3612 3697 4768 Size: 399 369 205 240 266 742 85 1071 Cuts at: 4768 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 369 266 240 205 85 BspEI T'CCGG_A Cuts at: 5214 5214 Size: 5551 BspHI T'CATG_A Cuts at: 3440 5170 3440 Size: 1730 3821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2764 2906 844 Size: 1920 142 3489 Fragments arranged by size: 3489 1920 142 BsrBI CCG'CTC Cuts at: 1394 1823 2643 3438 1394 Size: 429 820 795 3507 Fragments arranged by size: 3507 820 795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2194 4046 4220 65 Size: 1183 946 1852 174 1396 Fragments arranged by size: 1852 1396 1183 946 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2549 4195 4382 1700 Size: 110 739 1646 187 2869 Fragments arranged by size: 2869 1646 739 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5551 BssHII G'CGCG_C Cuts at: 1925 2261 2616 1925 Size: 336 355 4860 Fragments arranged by size: 4860 355 336 BssSI C'ACGA_G Cuts at: 3611 4623 3611 Size: 1012 4539 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5551 BstBI TT'CG_AA Cuts at: 2669 4355 2669 Size: 1686 3865 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5551 BstYI r'GATC_y Cuts at: 1972 2781 2881 3248 3633 3650 5102 5188 5200 Size: 809 100 367 385 17 1452 86 12 Cuts at: 5200 5546 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 809 385 367 346 100 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4060 Fragments arranged by size: 4060 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4812 Fragments arranged by size: 4812 683 56 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2855 2855 Size: 5551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2774 4558 809 Size: 507 1458 1784 1802 Fragments arranged by size: 1802 1784 1458 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2168 2242 2607 Size: 54 1425 50 275 18 338 74 365 Cuts at: 2607 2998 3887 5516 8 Size: 391 889 1629 43 Fragments arranged by size: 1629 1425 889 391 365 338 275 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2242 2998 1537 Size: 275 430 756 4090 Fragments arranged by size: 4090 756 430 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3481 1353 Size: 2128 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2192 4138 4512 4658 387 Size: 1064 138 603 1946 374 146 1280 Fragments arranged by size: 1946 1280 1064 603 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2405 2497 2711 3614 4211 4998 Size: 393 713 60 92 214 903 597 787 Cuts at: 4998 5266 1239 Size: 268 1524 Fragments arranged by size: 1524 903 787 713 597 393 268 214 92 60 EcoO109I rG'GnC_Cy Cuts at: 2320 2468 2320 Size: 148 5403 EcoRI G'AATT_C Cuts at: 2860 3238 2860 Size: 378 5173 FspI TGC'GCA Cuts at: 4057 4057 Size: 5551 HaeII r_GCGC'y Cuts at: 1678 2375 2550 2734 4698 1678 Size: 697 175 184 1964 2531 Fragments arranged by size: 2531 1964 697 184 175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2471 2503 4192 4224 4266 Size: 32 135 32 899 32 1689 32 42 Cuts at: 4266 4298 1373 Size: 32 2626 Fragments arranged by size: 2626 1689 899 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2710 2895 3141 669 Size: 988 281 772 185 246 3079 Fragments arranged by size: 3079 988 772 281 246 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 5551 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KasI G'GCGC_C Cuts at: 2546 2546 Size: 5551 KpnI G_GTAC'C Cuts at: 2629 2876 2629 Size: 247 5304 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 4665 4849 1305 Size: 1227 2133 184 2007 Fragments arranged by size: 2133 2007 1227 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3729 Fragments arranged by size: 3729 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3509 3868 4027 518 Size: 648 2343 359 159 2042 Fragments arranged by size: 2343 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2383 2613 2967 3644 4792 5037 1606 Size: 441 336 230 354 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 441 354 336 245 230 NaeI GCC'GGC Cuts at: 2551 2551 Size: 5551 NarI GG'CG_CC Cuts at: 2547 2547 Size: 5551 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4060 Fragments arranged by size: 4060 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NgoMIV G'CCGG_C Cuts at: 2549 2549 Size: 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2909 4454 1132 Size: 97 1680 1545 2229 Fragments arranged by size: 2229 1680 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5551 PciI A'CATG_T Cuts at: 4450 4450 Size: 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 2500 1108 Size: 1392 4159 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PmlI CAC'GTG Cuts at: 2250 2250 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3620 3652 5133 5165 3620 Size: 32 1481 32 4006 Fragments arranged by size: 4006 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2468 2468 Size: 5551 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5551 PsiI TTA'TAA Cuts at: 3121 3121 Size: 5551 PspOMI G'GGCC_C Cuts at: 2360 2360 Size: 5551 PstI C_TGCA'G Cuts at: 830 2903 830 Size: 2073 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SbfI CC_TGCA'GG Cuts at: 2903 2903 Size: 5551 ScaI AGT'ACT Cuts at: 3799 5511 3799 Size: 1712 3839 SfcI C'TryA_G Cuts at: 826 1045 2899 4034 4715 4906 826 Size: 219 1854 1135 681 191 1471 Fragments arranged by size: 1854 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2548 2548 Size: 5551 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 2549 1700 Size: 849 4702 SmaI CCC'GGG Cuts at: 1872 2032 2116 2352 2365 2878 1872 Size: 160 84 236 13 513 4545 Fragments arranged by size: 4545 513 236 160 84 13 SmlI C'TyrA_G Cuts at: 820 1017 1943 3655 4556 4818 820 Size: 197 926 1712 901 262 1553 Fragments arranged by size: 1712 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2909 1132 Size: 1777 3774 SspI AAT'ATT Cuts at: 5 52 3475 5 Size: 47 3423 2081 Fragments arranged by size: 3423 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5551 StyI C'CwwG_G Cuts at: 513 1065 2004 2990 513 Size: 552 939 986 3074 Fragments arranged by size: 3074 986 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3578 3595 3748 3933 4378 325 Size: 1255 1998 17 153 185 445 1498 Fragments arranged by size: 1998 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3797 5509 96 Size: 274 80 33 51 1443 1820 1712 138 Fragments arranged by size: 1820 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5511 5543 5511 Size: 32 5519 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5551 XmaI C'CCGG_G Cuts at: 1870 2030 2114 2350 2363 2876 1870 Size: 160 84 236 13 513 4545 Fragments arranged by size: 4545 513 236 160 84 13 XmnI GAAnn'nnTTC Cuts at: 3680 3680 Size: 5551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3359 276 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI