Restriction map for
pFN21AB8911
(Linear) (Six-base) MAPSORT of: KIBB8911.seq Check: 5381 from: 1 to: 750
KIBB8911.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 35 230 750
Size: 35 195 520
Fragments arranged by size:
520 195 35
Acc65I G'GTAC_C
Cuts at: 0 278 750
Size: 278 472
AfeI AGC'GCT
Cuts at: 0 565 750
Size: 565 185
AflIII A'CryG_T
Cuts at: 0 206 750
Size: 206 544
AleI CACnn'nnGTG
Cuts at: 0 56 750
Size: 56 694
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 598 630 750
Size: 598 32 120
Fragments arranged by size:
598 120 32
AlwNI CAG_nnn'CTG
Cuts at: 0 591 750
Size: 591 159
ApoI r'AATT_y
Cuts at: 0 476 750
Size: 476 274
AscI GG'CGCG_CC
Cuts at: 0 368 750
Size: 368 382
AvaI C'yCGr_G
Cuts at: 0 324 400 750
Size: 324 76 350
Fragments arranged by size:
350 324 76
BanI G'GyrC_C
Cuts at: 0 178 189 278 303 750
Size: 178 11 89 25 447
Fragments arranged by size:
447 178 89 25 11
BanII G_rGCy'C
Cuts at: 0 106 202 750
Size: 106 96 548
Fragments arranged by size:
548 106 96
BbeI G_GCGC'C
Cuts at: 0 182 193 307 750
Size: 182 11 114 443
Fragments arranged by size:
443 182 114 11
BglI GCCn_nnn'nGGC
Cuts at: 0 187 750
Size: 187 563
BlpI GC'TnA_GC
Cuts at: 0 19 196 750
Size: 19 177 554
Fragments arranged by size:
554 177 19
Bme1580I G_kGCm'C
Cuts at: 0 397 750
Size: 397 353
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 159 750
Size: 159 591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 154 316 613 750
Size: 56 98 162 297 137
Fragments arranged by size:
297 162 137 98 56
BsaHI Gr'CG_yC
Cuts at: 0 179 190 257 304 750
Size: 179 11 67 47 446
Fragments arranged by size:
446 179 67 47 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 598 628 750
Size: 598 30 122
Fragments arranged by size:
598 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 652 750
Size: 652 98
BseYI C'CCAG_C
Cuts at: 0 363 750
Size: 363 387
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 523 750
Size: 168 355 227
Fragments arranged by size:
355 227 168
BsiEI CG_ry'CG
Cuts at: 0 269 750
Size: 269 481
BsiHKAI G_wGCw'C
Cuts at: 0 106 131 750
Size: 106 25 619
Fragments arranged by size:
619 106 25
Bsp1286I G_dGCh'C
Cuts at: 0 106 131 202 397 750
Size: 106 25 71 195 353
Fragments arranged by size:
353 195 106 71 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 750
Size: 266 484
BsrFI r'CCGG_y
Cuts at: 0 168 264 300 704 750
Size: 168 96 36 404 46
Fragments arranged by size:
404 168 96 46 36
BssHII G'CGCG_C
Cuts at: 0 368 750
Size: 368 382
BssSI C'ACGA_G
Cuts at: 0 52 144 750
Size: 52 92 606
Fragments arranged by size:
606 92 52
DraIII CAC_nnn'GTG
Cuts at: 0 651 750
Size: 651 99
EaeI y'GGCC_r
Cuts at: 0 90 266 653 750
Size: 90 176 387 97
Fragments arranged by size:
387 176 97 90
EagI C'GGCC_G
Cuts at: 0 266 750
Size: 266 484
EarI CTCTTCn'nnn_
Cuts at: 0 555 750
Size: 555 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 367 516 723 750
Size: 367 149 207 27
Fragments arranged by size:
367 207 149 27
EcoICRI GAG'CTC
Cuts at: 0 104 750
Size: 104 646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 154 316 613 750
Size: 56 98 162 297 137
Fragments arranged by size:
297 162 137 98 56
HaeII r_GCGC'y
Cuts at: 0 169 182 193 307 567 750
Size: 169 13 11 114 260 183
Fragments arranged by size:
260 183 169 114 13 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 495 527 750
Size: 33 32 430 32 223
Fragments arranged by size:
430 223 33 32 32
HincII GTy'rAC
Cuts at: 0 36 750
Size: 36 714
KasI G'GCGC_C
Cuts at: 0 178 189 303 750
Size: 178 11 114 447
Fragments arranged by size:
447 178 114 11
KpnI G_GTAC'C
Cuts at: 0 282 750
Size: 282 468
MluI A'CGCG_T
Cuts at: 0 206 750
Size: 206 544
MscI TGG'CCA
Cuts at: 0 92 655 750
Size: 92 563 95
Fragments arranged by size:
563 95 92
MslI CAynn'nnrTG
Cuts at: 0 56 750
Size: 56 694
MspA1I CmG'CkG
Cuts at: 0 195 360 750
Size: 195 165 390
Fragments arranged by size:
390 195 165
NaeI GCC'GGC
Cuts at: 0 266 302 750
Size: 266 36 448
Fragments arranged by size:
448 266 36
NarI GG'CG_CC
Cuts at: 0 179 190 304 750
Size: 179 11 114 446
Fragments arranged by size:
446 179 114 11
NgoMIV G'CCGG_C
Cuts at: 0 264 300 750
Size: 264 36 450
Fragments arranged by size:
450 264 36
PasI CC'CwG_GG
Cuts at: 0 252 750
Size: 252 498
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 750
Size: 591 159
PfoI T'CCnGG_A
Cuts at: 0 211 553 750
Size: 211 342 197
Fragments arranged by size:
342 211 197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 750
Size: 598 32 120
Fragments arranged by size:
598 120 32
SacI G_AGCT'C
Cuts at: 0 106 750
Size: 106 644
SalI G'TCGA_C
Cuts at: 0 34 750
Size: 34 716
SapI GCTCTTCn'nnn_
Cuts at: 0 555 750
Size: 555 195
SfoI GGC'GCC
Cuts at: 0 180 191 305 750
Size: 180 11 114 445
Fragments arranged by size:
445 180 114 11
SgrAI Cr'CCGG_yG
Cuts at: 0 168 750
Size: 168 582
SmaI CCC'GGG
Cuts at: 0 326 750
Size: 326 424
TatI w'GTAC_w
Cuts at: 0 404 750
Size: 404 346
XmaI C'CCGG_G
Cuts at: 0 324 750
Size: 324 426
Enzymes that do cut:
AccI Acc65I AfeI AflIII AleI AloI AlwNI ApoI
AscI AvaI BanI BanII BbeI BglI BlpI Bme1580I
BplI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI HaeII Hin4I HincII
KasI KpnI MluI MscI MslI MspA1I NaeI NarI
NgoMIV PasI PflMI PfoI PpiI SacI SalI SapI
SfoI SgrAI SmaI TatI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AhdI AlfI
ApaI ApaLI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI
MmeI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI