(Circular) (Six-base) MAPSORT of: pFN21AB8897.seq Check: 1542 from: 1 to: 5377 pFN21AB8897 5377 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 278 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2385 2720 1937 Size: 448 335 4594 Fragments arranged by size: 4594 448 335 Acc65I G'GTAC_C Cuts at: 2698 2698 Size: 5377 AclI AA'CG_TT Cuts at: 1563 3504 3877 1563 Size: 1941 373 3063 Fragments arranged by size: 3063 1941 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3440 4824 5092 1632 Size: 1808 1384 268 1917 Fragments arranged by size: 1917 1808 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5180 AflIII A'CryG_T Cuts at: 1203 2465 4276 5253 1203 Size: 1262 1811 977 1327 Fragments arranged by size: 1811 1327 1262 977 AgeI A'CCGG_T Cuts at: 4208 4208 Size: 5377 AhdI GACnn_n'nnGTC Cuts at: 4106 4106 Size: 5377 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5377 AlwNI CAG_nnn'CTG Cuts at: 2479 3120 4692 2479 Size: 641 1572 3164 Fragments arranged by size: 3164 1572 641 ApaI G_GGCC'C Cuts at: 2436 2436 Size: 5377 ApaLI G'TGCA_C Cuts at: 3434 4590 3434 Size: 1156 4221 ApoI r'AATT_y Cuts at: 1493 2407 2686 2912 3064 5173 1493 Size: 914 279 226 152 2109 1697 Fragments arranged by size: 2109 1697 914 279 226 152 AseI AT'TA_AT Cuts at: 160 1032 3931 160 Size: 872 2899 1606 Fragments arranged by size: 2899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 AvaI C'yCGr_G Cuts at: 1870 1943 2306 2702 1870 Size: 73 363 396 4545 Fragments arranged by size: 4545 396 363 73 BamHI G'GATC_C Cuts at: 2707 3074 2707 Size: 367 5010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2212 2233 2279 2698 4153 Size: 325 217 627 425 21 46 419 1455 Cuts at: 4153 618 Size: 1842 Fragments arranged by size: 1842 1455 627 425 419 325 217 46 21 BanII G_rGCy'C Cuts at: 1391 2436 2696 1391 Size: 1045 260 4072 Fragments arranged by size: 4072 1045 260 BbeI G_GCGC'C Cuts at: 2216 2237 2283 2216 Size: 21 46 5310 Fragments arranged by size: 5310 46 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2521 2555 3568 3602 1903 Size: 34 584 34 1013 34 3678 Fragments arranged by size: 3678 1013 584 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3269 4479 3269 Size: 1210 4167 BclI T'GATC_A Cuts at: 1548 1548 Size: 5377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2134 2448 3988 Size: 107 122 71 631 605 462 314 1540 Cuts at: 3988 136 Size: 1525 Fragments arranged by size: 1540 1525 631 605 462 314 122 107 71 BglII A'GATC_T Cuts at: 5372 5372 Size: 5377 BlpI GC'TnA_GC Cuts at: 2627 2794 2627 Size: 167 5210 Bme1580I G_kGCm'C Cuts at: 1790 2436 3438 4594 1790 Size: 646 1002 1156 2573 Fragments arranged by size: 2573 1156 1002 646 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 4066 442 Size: 416 53 83 399 24 1691 958 1753 Fragments arranged by size: 1753 1691 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2507 4037 1239 Size: 1262 6 1530 2579 Fragments arranged by size: 2579 1530 1262 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3502 4367 4665 3502 Size: 865 298 4214 Fragments arranged by size: 4214 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4040 882 Size: 617 2541 2219 Fragments arranged by size: 2541 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3168 493 Size: 711 1964 2702 Fragments arranged by size: 2702 1964 711 BsaBI GATnn'nnATC Cuts at: 5371 5371 Size: 5377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2213 2234 2280 3184 Size: 53 83 186 936 680 21 46 904 Cuts at: 3184 3566 4201 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 904 680 635 382 186 83 53 46 21 BsaWI w'CCGG_w Cuts at: 2380 3810 4208 4482 4629 5040 2380 Size: 1430 398 274 147 411 2717 Fragments arranged by size: 2717 1430 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 2480 2510 1178 Size: 30 165 30 1005 30 42 30 4045 Fragments arranged by size: 4045 1005 165 42 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 2669 1186 Size: 1480 3 3894 Fragments arranged by size: 3894 1480 3 BseYI C'CCAG_C Cuts at: 2435 4580 5076 2435 Size: 2145 496 2736 Fragments arranged by size: 2736 2145 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4804 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2206 2443 2827 3588 4616 1540 Size: 275 186 205 237 384 761 1028 2301 Fragments arranged by size: 2301 1028 761 384 275 237 205 186 BsiHKAI G_wGCw'C Cuts at: 2375 3438 3523 4594 2375 Size: 1063 85 1071 3158 Fragments arranged by size: 3158 1071 1063 85 BsmI GAATG_Cn' Cuts at: 2888 2981 2888 Size: 93 5284 BsmBI CGTCTCn'nnnn_ Cuts at: 2640 2640 Size: 5377 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2375 2436 2696 3438 3523 4594 1391 Size: 399 585 61 260 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 585 399 260 85 61 BspEI T'CCGG_A Cuts at: 5040 5040 Size: 5377 BspHI T'CATG_A Cuts at: 3266 4996 3266 Size: 1730 3647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 844 Size: 1888 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2083 2210 3264 1394 Size: 429 260 127 1054 3507 Fragments arranged by size: 3507 1054 429 260 127 BsrDI GCAATG_nn' Cuts at: 65 1248 3872 4046 65 Size: 1183 2624 174 1396 Fragments arranged by size: 2624 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2185 2230 2236 2442 4021 4208 1700 Size: 110 375 45 6 206 1579 187 2869 Fragments arranged by size: 2869 1579 375 206 187 110 45 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5377 BssHII G'CGCG_C Cuts at: 1925 2328 1925 Size: 403 4974 BssSI C'ACGA_G Cuts at: 3437 4449 3437 Size: 1012 4365 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5377 BstBI TT'CG_AA Cuts at: 4181 4181 Size: 5377 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5377 BstYI r'GATC_y Cuts at: 1972 2707 3074 3459 3476 4928 5014 5026 5372 Size: 735 367 385 17 1452 86 12 346 Cuts at: 5372 1972 Size: 1977 Fragments arranged by size: 1977 1452 735 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2312 2390 513 Size: 552 1247 78 3500 Fragments arranged by size: 3500 1247 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4638 Fragments arranged by size: 4638 683 56 BtsI GCAGTG_nn' Cuts at: 2893 3687 3707 2893 Size: 794 20 4563 Fragments arranged by size: 4563 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5342 DraI TTT'AAA Cuts at: 2681 2681 Size: 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4384 809 Size: 507 3068 1802 Fragments arranged by size: 3068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2203 2440 Size: 54 1425 50 275 18 216 157 237 Cuts at: 2440 2824 3713 5342 8 Size: 384 889 1629 43 Fragments arranged by size: 1629 1425 889 384 275 237 216 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2203 2440 2824 1537 Size: 275 391 237 384 4090 Fragments arranged by size: 4090 391 384 275 237 EarI CTCTTCn'nnn_ Cuts at: 1353 2355 3307 1353 Size: 1002 952 3423 Fragments arranged by size: 3423 1002 952 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3964 4338 4484 387 Size: 1064 138 2375 374 146 1280 Fragments arranged by size: 2375 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 2507 3440 4037 4824 5092 1239 Size: 393 869 6 933 597 787 268 1524 Fragments arranged by size: 1524 933 869 787 597 393 268 6 EcoRI G'AATT_C Cuts at: 2407 2686 3064 2407 Size: 279 378 4720 Fragments arranged by size: 4720 378 279 FspI TGC'GCA Cuts at: 2269 3883 2269 Size: 1614 3763 HaeII r_GCGC'y Cuts at: 1678 2216 2237 2283 4524 1678 Size: 538 21 46 2241 2531 Fragments arranged by size: 2531 2241 538 46 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 4018 4050 4092 Size: 32 135 32 671 32 1743 32 42 Cuts at: 4092 4124 1373 Size: 32 2626 Fragments arranged by size: 2626 1743 671 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2721 2967 669 Size: 988 281 783 246 3079 Fragments arranged by size: 3079 988 783 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5377 HpaI GTT'AAC Cuts at: 2967 2967 Size: 5377 KasI G'GCGC_C Cuts at: 2212 2233 2279 2212 Size: 21 46 5310 Fragments arranged by size: 5310 46 21 KpnI G_GTAC'C Cuts at: 2702 2702 Size: 5377 MfeI C'AATT_G Cuts at: 2976 2976 Size: 5377 MluI A'CGCG_T Cuts at: 5253 5253 Size: 5377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 4491 4675 1305 Size: 996 2190 184 2007 Fragments arranged by size: 2190 2007 996 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 10 Size: 54 1425 343 216 3339 Fragments arranged by size: 3339 1425 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 3335 3694 3853 518 Size: 648 2169 359 159 2042 Fragments arranged by size: 2169 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 2110 2314 2401 2439 2793 3470 4618 Size: 446 58 204 87 38 354 677 1148 Cuts at: 4618 4863 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 446 354 245 204 87 58 38 NaeI GCC'GGC Cuts at: 2187 2232 2238 2444 2187 Size: 45 6 206 5120 Fragments arranged by size: 5120 206 45 6 NarI GG'CG_CC Cuts at: 2213 2234 2280 2213 Size: 21 46 5310 Fragments arranged by size: 5310 46 21 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4825 NdeI CA'TA_TG Cuts at: 387 387 Size: 5377 NgoMIV G'CCGG_C Cuts at: 2185 2230 2236 2442 2185 Size: 45 6 206 5120 Fragments arranged by size: 5120 206 45 6 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5377 NotI GC'GGCC_GC Cuts at: 2824 2824 Size: 5377 NspI r_CATG'y Cuts at: 1132 1229 2735 4280 1132 Size: 97 1506 1545 2229 Fragments arranged by size: 2229 1545 1506 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5377 PciI A'CATG_T Cuts at: 4276 4276 Size: 5377 PflMI CCAn_nnn'nTGG Cuts at: 1108 2479 1108 Size: 1371 4006 PmeI GTTT'AAAC Cuts at: 2681 2681 Size: 5377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3478 4959 4991 3446 Size: 32 1481 32 3832 Fragments arranged by size: 3832 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5377 PsiI TTA'TAA Cuts at: 2947 2947 Size: 5377 PspOMI G'GGCC_C Cuts at: 2432 2432 Size: 5377 PstI C_TGCA'G Cuts at: 830 2729 830 Size: 1899 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5377 SacII CC_GC'GG Cuts at: 2315 2315 Size: 5377 SalI G'TCGA_C Cuts at: 1936 2719 1936 Size: 783 4594 SapI GCTCTTCn'nnn_ Cuts at: 2355 2355 Size: 5377 SbfI CC_TGCA'GG Cuts at: 2729 2729 Size: 5377 ScaI AGT'ACT Cuts at: 3625 5337 3625 Size: 1712 3665 SfcI C'TryA_G Cuts at: 826 1045 2725 3860 4541 4732 826 Size: 219 1680 1135 681 191 1471 Fragments arranged by size: 1680 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2448 2448 Size: 5377 SfoI GGC'GCC Cuts at: 2214 2235 2281 2214 Size: 21 46 5310 Fragments arranged by size: 5310 46 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 SgrAI Cr'CCGG_yG Cuts at: 1700 2185 1700 Size: 485 4892 SmaI CCC'GGG Cuts at: 1872 2704 1872 Size: 832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3481 4382 4644 820 Size: 197 926 1538 901 262 1553 Fragments arranged by size: 1553 1538 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3168 493 Size: 2675 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5377 SphI G_CATG'C Cuts at: 1132 2735 1132 Size: 1603 3774 SspI AAT'ATT Cuts at: 5 52 3301 5 Size: 47 3249 2081 Fragments arranged by size: 3249 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5377 StyI C'CwwG_G Cuts at: 513 1065 2816 513 Size: 552 1751 3074 Fragments arranged by size: 3074 1751 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3404 3421 3574 3759 4204 325 Size: 1255 1824 17 153 185 445 1498 Fragments arranged by size: 1824 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3623 5335 96 Size: 274 80 33 51 1443 1646 1712 138 Fragments arranged by size: 1712 1646 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5337 5369 5337 Size: 32 5345 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5377 XbaI T'CTAG_A Cuts at: 2713 2713 Size: 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5377 XmaI C'CCGG_G Cuts at: 1870 2702 1870 Size: 832 4545 XmnI GAAnn'nnTTC Cuts at: 3506 3506 Size: 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 276 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SrfI SwaI XcmI