Restriction map for
pFN21AB8895
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI HphI ItaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
TfiI TseI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 449 GTAGAC GT^[AC][GT]AC
AflIII 503 ACATGT A^C[GA][CT]GT
AluI 76 AGCT AG^CT
AseI 57 ATTAAT AT^TAAT
AsnI 57 ATTAAT AT^TAAT
Asp700I 55 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 146 TGGCCA TGG^CCA
BamHI 394 GGATCC G^GATCC
BanII 177 GAGCCC G[GA]GC[CT]^C
BcnI 180 CCCGG CC^[GC]GG
BmyI 177 GAGCCC G[AGT]GC[ACT]^C
Bsp1286I 177 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 503 ACATGT A^CATGT
BstAPI 599 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 432 CCAGG CC^[AT]GG
Cac8I 599 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 117 GCGC GCG^C
DraI 355 TTTAAA TTT^AAA
EaeI 146 TGGCCA [CT]^GGCC[GA]
EarI 626 GAAGAG CTCTTC(1/4)
EcoRII 432 CCAGG ^CC[AT]GG
EcoRV 85 GATATC GAT^ATC
HaeII 116 AGCGCC [GA]GCGC^[CT]
HapII 181 CCGG C^CGG
HhaI 117 GCGC GCG^C
HinP1I 117 GCGC G^CGC
HinfI 547 GACTC G^A[ACGT]TC
HpaII 181 CCGG C^CGG
Ksp632I 626 GAAGAG CTCTTC(1/4)
MluNI 146 TGGCCA TGG^CCA
MscI 146 TGGCCA TGG^CCA
MslI 39 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 181 CCGG C^CGG
MvaI 432 CCAGG CC^[AT]GG
MwoI 599 GCAAGCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 180 CCCGG CC^[GC]GG
NdeI 218 CATATG CA^TATG
NspI 503 ACATGT [GA]CATG^[CT]
PleI 547 GACTC GAGTC(4/5)
PshBI 57 ATTAAT AT^TAAT
PstI 606 CTGCAG CTGCA^G
SfcI 606 CTGCAG C^T[GA][CT]AG
SpeI 283 ACTAGT A^CTAGT
StuI 131 AGGCCT AGG^CCT
Tsp45I 616 GTCAC ^GT[GC]AC
XmnI 55 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 196 AAATTC [GA]^AATT[CT]
472 GAATTT
AfaI 156 GTAC GT^AC
568 GTAC
ApoI 196 AAATTC [GA]^AATT[CT]
472 GAATTT
BfaI 284 CTAG C^TAG
596 CTAG
BssKI 180 CCCGG ^CC[ACGT]GG
432 CCAGG
BstF5I 183 GGATG GGATG(2/0)
435 GGATG
BstXI 384 CCAAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
432 CCAGGATGGTGG
BstYI 394 GGATCC [GA]^GATC[CT]
592 GGATCT
DdeI 90 CTTAG C^T[ACGT]AG
231 CTCAG
DpnII 395 GATC ^GATC
593 GATC
FokI 183 GGATG GGATG(9/13)
435 GGATG
MaeI 284 CTAG C^TAG
596 CTAG
MaeIII 616 GTCAC ^GT[ACGT]AC
640 GTTAC
MboI 395 GATC ^GATC
593 GATC
MboII 511 GAAGA GAAGA(8/7)
626 GAAGA
MflI 394 GGATCC [GA]^GATC[CT]
592 GGATCT
NdeII 395 GATC ^GATC
593 GATC
NlaIV 394 GGATCC GG[ACGT]^[ACGT]CC
442 GGTTCC
RsaI 156 GTAC GT^AC
568 GTAC
Sau3AI 395 GATC ^GATC
593 GATC
ScrFI 180 CCCGG CC^[ACGT]GG
432 CCAGG
XhoII 394 GGATCC [GA]^GATC[CT]
592 GGATCT
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AlwI BsrI HaeIII MnlI MseI
NlaIII SfaNI Tru9I Tsp509I TspRI