Restriction map for
pFN21AB8890
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI Fnu4HI FseI FspI HaeII
HapII Hin1I HincII HindII HindIII
HinfI HpaI HpaII ItaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI TaqI TfiI TseI
TthHB8I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflII 178 CTTAAG C^TTAAG
AluI 575 AGCT AG^CT
ApaI 212 GGGCCC GGGCC^C
Asp700I 558 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 538 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 375 GGACC G^G[AT]CC
BanII 212 GGGCCC G[GA]GC[CT]^C
BfaI 595 CTAG C^TAG
BfrI 178 CTTAAG C^TTAAG
BpmI 11 CTCCAG CTGGAG(16/14)
BsaI 543 GGTCTC GGTCTC(1/5)
Bsp1407I 434 TGTACA T^GTACA
BspHI 419 TCATGA T^CATGA
BsrDI 321 CATTGC GCAATG(2/0)
BsrGI 434 TGTACA T^GTACA
BssSI 502 CTCGTG CACGAG(-5/-1)
CfoI 255 GCGC GCG^C
DpnII 85 GATC ^GATC
DraII 211 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 211 AGGGCCC [GA]G^G[ACGT]CC[CT]
FauI 526 CCCGC CCCGC(4/6)
HgaI 218 GACGC GACGC(5/10)
HhaI 255 GCGC GCG^C
HinP1I 255 GCGC G^CGC
MaeI 595 CTAG C^TAG
MboI 85 GATC ^GATC
MspA1I 527 CCGCTG C[AC]G^C[GT]G
NdeII 85 GATC ^GATC
PflFI 538 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 419 TCATGA T^CATGA
Sau3AI 85 GATC ^GATC
SmlI 178 CTTAAG C^T[CT][GA]AG
SspBI 434 TGTACA T^GTACA
StuI 482 AGGCCT AGG^CCT
Tsp509I 509 AATT ^AATT
Tth111I 538 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 558 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AfaI 60 GTAC GT^AC
435 GTAC
BsmFI 264 GGGAC GGGAC(10/14)
396 GGGAC
BsrI 34 ACTGG ACTGG(1/-1)
13 CCAGT
BstF5I 105 CATCC GGATG(2/0)
450 CATCC
EcoNI 63 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
174 CCTCCTTAAGG
FokI 105 CATCC GGATG(9/13)
450 CATCC
MboII 423 GAAGA GAAGA(8/7)
558 GAAGA
PflMI 159 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
294 CCATGCCCTGG
RsaI 60 GTAC GT^AC
435 GTAC
TspRI 33 CACTG CA[GC]TG[ACGT][ACGT]^
477 CACTG
Van91I 159 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
294 CCATGCCCTGG
XcmI 44 CCATTAACATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
108 CCACCGCCTTCGTGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI BanI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI EcoRII HaeIII
HphI MaeIII MnlI MseI MslI
MvaI NlaIII NlaIV Sau96I ScrFI
SfaNI Tru9I Tsp45I