Restriction map for
pFN21AB8887
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AclI AcsI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BglII BlnI BsaAI
BsaBI BsaI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BsrI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MfeI
MluI MslI MunI NcoI NdeI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AatII 448 GACGTC GACGT^C
AccIII 216 TCCGGA T^CCGGA
AfaI 324 GTAC GT^AC
AflII 39 CTTAAG C^TTAAG
AlwI 517 GATCC GGATC(4/5)
AlwNI 286 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 228 GGGCCC GGGCC^C
AspHI 80 GAGCAC G[AT]GC[AT]^C
BalI 371 TGGCCA TGG^CCA
BanI 330 GGCGCC G^G[CT][GA]CC
BbeI 330 GGCGCC GGCGC^C
BbsI 313 GTCTTC GAAGAC(2/6)
BfrI 39 CTTAAG C^TTAAG
BglI 536 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 491 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 491 GCTTAGC GC^T[ACGT]AGC
BpuAI 313 GTCTTC GAAGAC(2/6)
BsaWI 216 TCCGGA [AT]^CCGG[AT]
BseAI 216 TCCGGA T^CCGGA
BsgI 486 GTGCAG GTGCAG(16/14)
BsiEI 243 CGGCCG CG[GA][CT]^CG
BsiHKAI 80 GAGCAC G[AT]GC[AT]^C
BsmAI 344 GAGAC GTCTC(1/5)
BsmBI 344 GAGACG CGTCTC(1/5)
BspEI 216 TCCGGA T^CCGGA
BsrBI 301 GAGCGG CCGCTC(-3/-3)
BstF5I 54 CATCC GGATG(2/0)
BstYI 516 AGATCC [GA]^GATC[CT]
CelII 491 GCTTAGC GC^T[ACGT]AGC
DdeI 492 CTTAG C^T[ACGT]AG
DpnII 517 GATC ^GATC
DraII 228 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 168 CCGCGG C^C[GA][CT]GG
EagI 243 CGGCCG C^GGCCG
EclXI 243 CGGCCG C^GGCCG
Eco52I 243 CGGCCG C^GGCCG
EcoO109I 228 GGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 54 CATCC GGATG(9/13)
HphI 275 TCACC GGTGA(8/7)
KasI 330 GGCGCC G^GCGCC
KspI 168 CCGCGG CCGC^GG
MboI 517 GATC ^GATC
MboII 314 TCTTC GAAGA(8/7)
MflI 516 AGATCC [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MroI 216 TCCGGA T^CCGGA
MscI 371 TGGCCA TGG^CCA
MseI 40 TTAA T^TAA
NaeI 541 GCCGGC GCC^GGC
NarI 330 GGCGCC GG^CGCC
NdeII 517 GATC ^GATC
NgoMIV 541 GCCGGC G^CCGGC
NheI 351 GCTAGC G^CTAGC
NlaIII 69 CATG CATG^
PstI 190 CTGCAG CTGCA^G
RsaI 324 GTAC GT^AC
SacII 168 CCGCGG CCGC^GG
Sau3AI 517 GATC ^GATC
SfcI 190 CTGCAG C^T[GA][CT]AG
SfiI 535 GGCCCTGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 330 GGCGCC GGC^GCC
SpeI 154 ACTAGT A^CTAGT
Tru9I 40 TTAA T^TAA
XcmI 374 CCAACGCGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 516 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 330 GGCGCC G[GA]^CG[CT]C
448 GACGTC
BanII 16 GAGCCC G[GA]GC[CT]^C
228 GGGCCC
BsaHI 330 GGCGCC G[GA]^CG[CT]C
448 GACGTC
BspMI 211 GCAGGT ACCTGC(4/8)
381 GCAGGT
BsrFI 404 ACCGGC [GA]^CCGG[CT]
541 GCCGGC
BstAPI 82 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
282 GCAACAGGTGC
Cfr10I 404 ACCGGC [GA]^CCGG[CT]
541 GCCGGC
EaeI 243 CGGCCG [CT]^GGCC[GA]
371 TGGCCA
FauI 63 CCCGC CCCGC(4/6)
207 GCGGG
Hin1I 330 GGCGCC G[GA]^CG[CT]C
448 GACGTC
MaeII 124 ACGT A^CGT
449 ACGT
MaeIII 261 GTCAC ^GT[ACGT]AC
388 GTCAC
MspA1I 130 CCGCTG C[AC]G^C[GT]G
168 CCGCGG
SfaNI 438 GATGC GCATC(5/9)
44 GCATC
SmlI 39 CTTAAG C^T[CT][GA]AG
418 CTCAAG
Tsp45I 261 GTCAC ^GT[GC]AC
388 GTCAC
TspRI 267 CACTG CA[GC]TG[ACGT][ACGT]^
497 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr13I EcoRII
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MaeI MnlI MspI MvaI MvnI
MwoI NciI NlaIV PleI Sau96I
ScrFI TseI