(Circular) (Six-base) MAPSORT of: pFN21AB8887.seq Check: 9586 from: 1 to: 5299 pFN21AB8887 5299 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2377 2377 Size: 5299 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2457 3109 278 Size: 53 83 186 936 921 652 2468 Fragments arranged by size: 2468 936 921 652 186 83 53 AccI GT'mk_AC Cuts at: 1937 2642 1937 Size: 705 4594 Acc65I G'GTAC_C Cuts at: 2620 2620 Size: 5299 AclI AA'CG_TT Cuts at: 1563 3426 3799 1563 Size: 1863 373 3063 Fragments arranged by size: 3063 1863 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2226 3362 4746 5014 1632 Size: 594 1136 1384 268 1917 Fragments arranged by size: 1917 1384 1136 594 268 AflII C'TTAA_G Cuts at: 820 1017 2044 820 Size: 197 1027 4075 Fragments arranged by size: 4075 1027 197 AflIII A'CryG_T Cuts at: 1203 4198 5175 1203 Size: 2995 977 1327 Fragments arranged by size: 2995 1327 977 AgeI A'CCGG_T Cuts at: 4130 4130 Size: 5299 AhdI GACnn_n'nnGTC Cuts at: 4028 4028 Size: 5299 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5299 AlwNI CAG_nnn'CTG Cuts at: 2296 3042 4614 2296 Size: 746 1572 2981 Fragments arranged by size: 2981 1572 746 ApaI G_GGCC'C Cuts at: 2237 2237 Size: 5299 ApaLI G'TGCA_C Cuts at: 3356 4512 3356 Size: 1156 4143 ApoI r'AATT_y Cuts at: 1493 2608 2834 2986 5095 1493 Size: 1115 226 152 2109 1697 Fragments arranged by size: 2109 1697 1115 226 152 AseI AT'TA_AT Cuts at: 160 1032 3853 160 Size: 872 2821 1606 Fragments arranged by size: 2821 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5299 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2082 2507 2624 1870 Size: 73 68 71 425 117 4545 Fragments arranged by size: 4545 425 117 73 71 68 BamHI G'GATC_C Cuts at: 2629 2996 2629 Size: 367 4932 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2335 2620 4075 618 Size: 325 217 627 548 285 1455 1842 Fragments arranged by size: 1842 1455 627 548 325 285 217 BanII G_rGCy'C Cuts at: 1391 2025 2237 2618 1391 Size: 634 212 381 4072 Fragments arranged by size: 4072 634 381 212 BbeI G_GCGC'C Cuts at: 2339 2339 Size: 5299 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2311 928 Size: 971 412 3916 Fragments arranged by size: 3916 971 412 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3490 3524 1903 Size: 34 1553 34 3678 Fragments arranged by size: 3678 1553 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3191 4401 3191 Size: 1210 4089 BclI T'GATC_A Cuts at: 1548 1548 Size: 5299 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2547 3910 136 Size: 107 122 71 631 605 875 1363 1525 Fragments arranged by size: 1525 1363 875 631 605 122 107 71 BglII A'GATC_T Cuts at: 5294 5294 Size: 5299 BlpI GC'TnA_GC Cuts at: 2497 2716 2497 Size: 219 5080 Bme1580I G_kGCm'C Cuts at: 1790 2237 3360 4516 1790 Size: 447 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 447 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5299 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3030 3988 442 Size: 416 53 83 399 24 1613 958 1753 Fragments arranged by size: 1753 1613 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2360 1056 Size: 1304 3995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2438 2438 Size: 5299 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2318 2324 2502 3959 1239 Size: 929 150 6 178 1457 2579 Fragments arranged by size: 2579 1457 929 178 150 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2408 3424 4289 4587 2408 Size: 1016 865 298 3120 Fragments arranged by size: 3120 1016 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3962 882 Size: 617 2463 2219 Fragments arranged by size: 2463 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3090 493 Size: 711 1886 2702 Fragments arranged by size: 2702 1886 711 BsaBI GATnn'nnATC Cuts at: 5293 5293 Size: 5299 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2336 2454 3106 3488 Size: 53 83 186 936 803 118 652 382 Cuts at: 3488 4123 275 Size: 635 1451 Fragments arranged by size: 1451 936 803 652 635 382 186 118 83 53 BsaWI w'CCGG_w Cuts at: 2221 3732 4130 4404 4551 4962 2221 Size: 1511 398 274 147 411 2558 Fragments arranged by size: 2558 1511 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5074 Fragments arranged by size: 5074 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2453 2594 1186 Size: 979 288 141 3891 Fragments arranged by size: 3891 979 288 141 BseYI C'CCAG_C Cuts at: 2230 4502 4998 2230 Size: 2272 496 2531 Fragments arranged by size: 2531 2272 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2512 1163 Size: 573 776 3950 Fragments arranged by size: 3950 776 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2251 2749 3510 4538 1540 Size: 275 186 250 498 761 1028 2301 Fragments arranged by size: 2301 1028 761 498 275 250 186 BsiHKAI G_wGCw'C Cuts at: 2089 3360 3445 4516 2089 Size: 1271 85 1071 2872 Fragments arranged by size: 2872 1271 1071 85 BsmI GAATG_Cn' Cuts at: 2810 2903 2810 Size: 93 5206 BsmBI CGTCTCn'nnnn_ Cuts at: 2343 2343 Size: 5299 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2089 2237 2618 3360 3445 4516 Size: 399 235 64 148 381 742 85 1071 Cuts at: 4516 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 381 235 148 85 64 BspEI T'CCGG_A Cuts at: 2221 4962 2221 Size: 2741 2558 BspHI T'CATG_A Cuts at: 3188 4918 3188 Size: 1730 3569 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 2377 2654 844 Size: 1363 170 277 3489 Fragments arranged by size: 3489 1363 277 170 BsrBI CCG'CTC Cuts at: 1394 1823 2308 3186 1394 Size: 429 485 878 3507 Fragments arranged by size: 3507 878 485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3794 3968 65 Size: 1183 2546 174 1396 Fragments arranged by size: 2546 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2409 2546 3943 4130 1700 Size: 110 599 137 1397 187 2869 Fragments arranged by size: 2869 1397 599 187 137 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5299 BssHII G'CGCG_C Cuts at: 1925 2142 2186 2188 2446 1925 Size: 217 44 2 258 4778 Fragments arranged by size: 4778 258 217 44 2 BssSI C'ACGA_G Cuts at: 3359 4371 3359 Size: 1012 4287 BstAPI GCAn_nnn'nTGC Cuts at: 794 2093 2293 794 Size: 1299 200 3800 Fragments arranged by size: 3800 1299 200 BstBI TT'CG_AA Cuts at: 4103 4103 Size: 5299 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5299 BstYI r'GATC_y Cuts at: 1972 2521 2629 2996 3381 3398 4850 4936 4948 Size: 549 108 367 385 17 1452 86 12 Cuts at: 4948 5294 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 549 385 367 346 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2173 513 Size: 552 1108 3639 Fragments arranged by size: 3639 1108 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2035 2445 487 Size: 683 56 809 410 3341 Fragments arranged by size: 3341 809 683 410 56 BtsI GCAGTG_nn' Cuts at: 2271 2501 2815 3609 3629 2271 Size: 230 314 794 20 3941 Fragments arranged by size: 3941 794 314 230 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5299 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5264 DraI TTT'AAA Cuts at: 2603 2603 Size: 5299 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4306 809 Size: 507 2990 1802 Fragments arranged by size: 2990 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2376 2746 Size: 54 1425 50 275 18 418 128 370 Cuts at: 2746 3635 5264 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 418 370 275 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2248 2746 1537 Size: 275 436 498 4090 Fragments arranged by size: 4090 498 436 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3229 1353 Size: 1876 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3886 4260 4406 387 Size: 1064 138 2297 374 146 1280 Fragments arranged by size: 2297 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2226 2318 2324 2502 3362 3959 Size: 393 536 58 92 6 178 860 597 Cuts at: 3959 4746 5014 1239 Size: 787 268 1524 Fragments arranged by size: 1524 860 787 597 536 393 268 178 92 58 6 EcoO109I rG'GnC_Cy Cuts at: 2234 2234 Size: 5299 EcoRI G'AATT_C Cuts at: 2608 2986 2608 Size: 378 4921 FspI TGC'GCA Cuts at: 3805 3805 Size: 5299 HaeII r_GCGC'y Cuts at: 1678 2314 2339 2517 4446 1678 Size: 636 25 178 1929 2531 Fragments arranged by size: 2531 1929 636 178 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 3940 3972 4014 Size: 32 135 32 872 32 1464 32 42 Cuts at: 4014 4046 1373 Size: 32 2626 Fragments arranged by size: 2626 1464 872 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2643 2889 669 Size: 988 281 705 246 3079 Fragments arranged by size: 3079 988 705 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5299 HpaI GTT'AAC Cuts at: 2889 2889 Size: 5299 KasI G'GCGC_C Cuts at: 2335 2335 Size: 5299 KpnI G_GTAC'C Cuts at: 2624 2624 Size: 5299 MfeI C'AATT_G Cuts at: 2898 2898 Size: 5299 MluI A'CGCG_T Cuts at: 5175 5175 Size: 5299 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2150 4413 4597 1305 Size: 845 2263 184 2007 Fragments arranged by size: 2263 2007 845 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 2931 Fragments arranged by size: 2931 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3257 3616 3775 518 Size: 648 2091 359 159 2042 Fragments arranged by size: 2091 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2175 2715 3392 4540 4785 1606 Size: 531 38 540 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 540 531 245 38 NaeI GCC'GGC Cuts at: 2548 2548 Size: 5299 NarI GG'CG_CC Cuts at: 2336 2336 Size: 5299 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4747 NdeI CA'TA_TG Cuts at: 387 387 Size: 5299 NgoMIV G'CCGG_C Cuts at: 2546 2546 Size: 5299 NheI G'CTAG_C Cuts at: 1052 2356 1052 Size: 1304 3995 NotI GC'GGCC_GC Cuts at: 2746 2746 Size: 5299 NspI r_CATG'y Cuts at: 1132 1229 2657 4202 1132 Size: 97 1428 1545 2229 Fragments arranged by size: 2229 1545 1428 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5299 PciI A'CATG_T Cuts at: 4198 4198 Size: 5299 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5299 PmeI GTTT'AAAC Cuts at: 2603 2603 Size: 5299 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3368 3400 4881 4913 3368 Size: 32 1481 32 3754 Fragments arranged by size: 3754 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5299 PsiI TTA'TAA Cuts at: 2869 2869 Size: 5299 PspOMI G'GGCC_C Cuts at: 2233 2233 Size: 5299 PstI C_TGCA'G Cuts at: 830 2199 2651 830 Size: 1369 452 3478 Fragments arranged by size: 3478 1369 452 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5299 SacII CC_GC'GG Cuts at: 2176 2176 Size: 5299 SalI G'TCGA_C Cuts at: 1936 2641 1936 Size: 705 4594 SbfI CC_TGCA'GG Cuts at: 2651 2651 Size: 5299 ScaI AGT'ACT Cuts at: 3547 5259 3547 Size: 1712 3587 SfcI C'TryA_G Cuts at: 826 1045 2195 2647 3782 4463 4654 826 Size: 219 1150 452 1135 681 191 1471 Fragments arranged by size: 1471 1150 1135 681 452 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2547 2547 Size: 5299 SfoI GGC'GCC Cuts at: 2337 2337 Size: 5299 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5299 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5299 SmaI CCC'GGG Cuts at: 1872 2626 1872 Size: 754 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2044 2423 3403 4304 4566 820 Size: 197 926 101 379 980 901 262 1553 Fragments arranged by size: 1553 980 926 901 379 262 197 101 SnaBI TAC'GTA Cuts at: 493 3090 493 Size: 2597 2702 SpeI A'CTAG_T Cuts at: 152 2159 152 Size: 2007 3292 SphI G_CATG'C Cuts at: 1132 2657 1132 Size: 1525 3774 SspI AAT'ATT Cuts at: 5 52 3223 5 Size: 47 3171 2081 Fragments arranged by size: 3171 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5299 StyI C'CwwG_G Cuts at: 513 1065 2738 513 Size: 552 1673 3074 Fragments arranged by size: 3074 1673 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3326 3343 3496 3681 4126 325 Size: 1255 1746 17 153 185 445 1498 Fragments arranged by size: 1746 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3545 5257 96 Size: 274 80 33 51 1443 1568 1712 138 Fragments arranged by size: 1712 1568 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2474 2506 5259 5291 2474 Size: 32 2753 32 2482 Fragments arranged by size: 2753 2482 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5299 XbaI T'CTAG_A Cuts at: 2635 2635 Size: 5299 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 2386 Size: 5299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5299 XmaI C'CCGG_G Cuts at: 1870 2624 1870 Size: 754 4545 XmnI GAAnn'nnTTC Cuts at: 3428 3428 Size: 5299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2455 3107 276 Size: 53 83 186 936 921 652 2468 Fragments arranged by size: 2468 936 921 652 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI