Restriction map for
pFN21AB8887
(Linear) (Six-base) MAPSORT of: KIBB8887.seq Check: 569 from: 1 to: 594
KIBB8887.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 372 594
Size: 372 222
AatII G_ACGT'C
Cuts at: 0 452 594
Size: 452 142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 594
Size: 221 373
AflII C'TTAA_G
Cuts at: 0 39 594
Size: 39 555
AlwNI CAG_nnn'CTG
Cuts at: 0 291 594
Size: 291 303
ApaI G_GGCC'C
Cuts at: 0 232 594
Size: 232 362
AvaI C'yCGr_G
Cuts at: 0 6 77 502 594
Size: 6 71 425 92
Fragments arranged by size:
425 92 71 6
BanI G'GyrC_C
Cuts at: 0 330 594
Size: 330 264
BanII G_rGCy'C
Cuts at: 0 20 232 594
Size: 20 212 362
Fragments arranged by size:
362 212 20
BbeI G_GCGC'C
Cuts at: 0 334 594
Size: 334 260
BbsI GAAGACnn'nnnn_
Cuts at: 0 306 594
Size: 306 288
BglI GCCn_nnn'nGGC
Cuts at: 0 542 594
Size: 542 52
BlpI GC'TnA_GC
Cuts at: 0 492 594
Size: 492 102
Bme1580I G_kGCm'C
Cuts at: 0 232 594
Size: 232 362
BmtI G_CTAG'C
Cuts at: 0 355 594
Size: 355 239
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 594
Size: 433 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 313 319 497 594
Size: 163 150 6 178 97
Fragments arranged by size:
178 163 150 97 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 594
Size: 403 191
BsaHI Gr'CG_yC
Cuts at: 0 331 449 594
Size: 331 118 145
Fragments arranged by size:
331 145 118
BsaWI w'CCGG_w
Cuts at: 0 216 594
Size: 216 378
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 448 589 594
Size: 160 288 141 5
Fragments arranged by size:
288 160 141 5
BseYI C'CCAG_C
Cuts at: 0 225 594
Size: 225 369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 594
Size: 507 87
BsiEI CG_ry'CG
Cuts at: 0 246 594
Size: 246 348
BsiHKAI G_wGCw'C
Cuts at: 0 84 594
Size: 84 510
BsmBI CGTCTCn'nnnn_
Cuts at: 0 338 594
Size: 338 256
Bsp1286I G_dGCh'C
Cuts at: 0 20 84 232 594
Size: 20 64 148 362
Fragments arranged by size:
362 148 64 20
BspEI T'CCGG_A
Cuts at: 0 216 594
Size: 216 378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 372 594
Size: 202 170 222
Fragments arranged by size:
222 202 170
BsrBI CCG'CTC
Cuts at: 0 303 594
Size: 303 291
BsrFI r'CCGG_y
Cuts at: 0 404 541 594
Size: 404 137 53
Fragments arranged by size:
404 137 53
BssHII G'CGCG_C
Cuts at: 0 137 181 183 441 594
Size: 137 44 2 258 153
Fragments arranged by size:
258 153 137 44 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 88 288 594
Size: 88 200 306
Fragments arranged by size:
306 200 88
BstYI r'GATC_y
Cuts at: 0 516 594
Size: 516 78
BtgI C'CryG_G
Cuts at: 0 168 594
Size: 168 426
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 30 440 594
Size: 30 410 154
Fragments arranged by size:
410 154 30
BtsI GCAGTG_nn'
Cuts at: 0 266 496 594
Size: 266 230 98
Fragments arranged by size:
266 230 98
EaeI y'GGCC_r
Cuts at: 0 243 371 594
Size: 243 128 223
Fragments arranged by size:
243 223 128
EagI C'GGCC_G
Cuts at: 0 243 594
Size: 243 351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 221 313 319 497 594
Size: 163 58 92 6 178 97
Fragments arranged by size:
178 163 97 92 58 6
EcoO109I rG'GnC_Cy
Cuts at: 0 229 594
Size: 229 365
HaeII r_GCGC'y
Cuts at: 0 309 334 512 594
Size: 309 25 178 82
Fragments arranged by size:
309 178 82 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 594
Size: 439 32 123
Fragments arranged by size:
439 123 32
KasI G'GCGC_C
Cuts at: 0 330 594
Size: 330 264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 594
Size: 145 449
MscI TGG'CCA
Cuts at: 0 373 594
Size: 373 221
MspA1I CmG'CkG
Cuts at: 0 132 170 594
Size: 132 38 424
Fragments arranged by size:
424 132 38
NaeI GCC'GGC
Cuts at: 0 543 594
Size: 543 51
NarI GG'CG_CC
Cuts at: 0 331 594
Size: 331 263
NgoMIV G'CCGG_C
Cuts at: 0 541 594
Size: 541 53
NheI G'CTAG_C
Cuts at: 0 351 594
Size: 351 243
PspOMI G'GGCC_C
Cuts at: 0 228 594
Size: 228 366
PstI C_TGCA'G
Cuts at: 0 194 594
Size: 194 400
SacII CC_GC'GG
Cuts at: 0 171 594
Size: 171 423
SfcI C'TryA_G
Cuts at: 0 190 594
Size: 190 404
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 542 594
Size: 542 52
SfoI GGC'GCC
Cuts at: 0 332 594
Size: 332 262
SmlI C'TyrA_G
Cuts at: 0 39 418 594
Size: 39 379 176
Fragments arranged by size:
379 176 39
SpeI A'CTAG_T
Cuts at: 0 154 594
Size: 154 440
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 469 501 594
Size: 469 32 93
Fragments arranged by size:
469 93 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 594
Size: 381 213
ZraI GAC'GTC
Cuts at: 0 450 594
Size: 450 144
Enzymes that do cut:
AarI AatII AcuI AflII AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI BlpI Bme1580I BmtI BplI
BpmI BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstYI BtgI BtgZI BtsI EaeI EagI Eco57MI
EcoO109I HaeII Hin4I KasI MmeI MscI MspA1I NaeI
NarI NgoMIV NheI PspOMI PstI SacII SfcI SfiI
SfoI SmlI SpeI TstI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmrI Bpu10I BsaI BsaAI BsaBI BsaXI BsiWI
BsmI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MslI
NcoI NdeI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI StuI StyI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI XmnI