Restriction map for
pFN21AB8880
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# NO CUT ENZYMES ( 198 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BclI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HaeIII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI ItaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TseI Tsp45I Tth111I TthHB8I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 190 GTAGAC GT^[AC][GT]AC
AciI 367 GCGG CCGC(-3/-1)
AcsI 13 GAATTT [GA]^AATT[CT]
AlwI 79 GGATC GGATC(4/5)
ApoI 13 GAATTT [GA]^AATT[CT]
Asp700I 286 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 85 GGTCC G^G[AT]CC
BbsI 238 GAAGAC GAAGAC(2/6)
BciVI 336 GGATAC GTATCC(6/5)
BcnI 327 CCCGG CC^[GC]GG
BfaI 472 CTAG C^TAG
BglII 488 AGATCT A^GATCT
BpuAI 238 GAAGAC GAAGAC(2/6)
BsaJI 326 CCCCGG C^C[ACGT][ACGT]GG
BsrI 254 ACTGG ACTGG(1/-1)
BstNI 76 CCTGG CC^[AT]GG
BstYI 488 AGATCT [GA]^GATC[CT]
Cac8I 33 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 85 GGTCC G^G[ACGT]CC
EcoNI 76 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 76 CCTGG ^CC[AT]GG
HapII 328 CCGG C^CGG
HgaI 56 GCGTC GACGC(5/10)
HinfI 150 GAATC G^A[ACGT]TC
HpaII 328 CCGG C^CGG
MaeI 472 CTAG C^TAG
MaeII 97 ACGT A^CGT
MflI 488 AGATCT [GA]^GATC[CT]
MnlI 413 GAGG CCTC(7/6)
MspI 328 CCGG C^CGG
MvaI 76 CCTGG CC^[AT]GG
NciI 327 CCCGG CC^[GC]GG
NlaIII 161 CATG CATG^
PstI 49 CTGCAG CTGCA^G
Sau96I 85 GGTCC G^G[ACGT]CC
SfaNI 245 GCATC GCATC(5/9)
TfiI 150 GAATC G^A[AT]TC
XbaI 471 TCTAGA T^CTAGA
XhoII 488 AGATCT [GA]^GATC[CT]
XmnI 286 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BssKI 76 CCTGG ^CC[ACGT]GG
327 CCCGG
BstF5I 330 GGATG GGATG(2/0)
461 GGATG
DdeI 38 CTCAG C^T[ACGT]AG
126 CTCAG
DpnII 80 GATC ^GATC
489 GATC
FokI 330 GGATG GGATG(9/13)
461 GGATG
MaeIII 268 GTAAC ^GT[ACGT]AC
478 GTTAC
MboI 80 GATC ^GATC
489 GATC
MboII 238 GAAGA GAAGA(8/7)
260 GAAGA
MseI 176 TTAA T^TAA
251 TTAA
NdeII 80 GATC ^GATC
489 GATC
PflMI 70 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
216 CCAAGTGATGG
Sau3AI 80 GATC ^GATC
489 GATC
ScrFI 76 CCTGG CC^[ACGT]GG
327 CCCGG
SfcI 49 CTGCAG C^T[GA][CT]AG
61 CTACAG
Tru9I 176 TTAA T^TAA
251 TTAA
TspRI 52 CAGTG CA[GC]TG[ACGT][ACGT]^
195 CACTG
Van91I 70 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
216 CCAAGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
BsiYI BslI Tsp509I