Restriction map for
pFN21AB8873
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaII AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI Ksp632I
MaeI MaeII MamI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I TspRI Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 378 GGCGCC G[GA]^CG[CT]C
AfaI 330 GTAC GT^AC
AlwNI 115 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 307 GAGCTC G[AT]GC[AT]^C
AspI 90 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 386 CCCGGG C^[CT]CG[GA]G
BbeI 378 GGCGCC GGCGC^C
BsaHI 378 GGCGCC G[GA]^CG[CT]C
BsiEI 481 CGATCG CG[GA][CT]^CG
BsiHKAI 307 GAGCTC G[AT]GC[AT]^C
BsmFI 421 GTCCC GGGAC(10/14)
BsoBI 386 CCCGGG C^[CT]CG[GA]G
BspMI 119 ACCTGC ACCTGC(4/8)
BstEII 87 GGTGACC G^GT[ACGT]ACC
BstPI 87 GGTGACC G^GT[ACGT]ACC
DdeI 282 CTCAG C^T[ACGT]AG
EaeI 434 TGGCCG [CT]^GGCC[GA]
EcoNI 129 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 87 GGTGACC G^GT[ACGT]ACC
EcoT14I 134 CCAAGG C^C[AT][AT]GG
Hin1I 378 GGCGCC G[GA]^CG[CT]C
KasI 378 GGCGCC G^GCGCC
KspI 183 CCGCGG CCGC^GG
MslI 93 CATGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 378 GGCGCC GG^CGCC
PflFI 90 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 481 CGATCG CGAT^CG
RsaI 330 GTAC GT^AC
SacI 307 GAGCTC GAGCT^C
SacII 183 CCGCGG CCGC^GG
SfaNI 475 GATGC GCATC(5/9)
SfoI 378 GGCGCC GGC^GCC
SgrAI 163 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 386 CCCGGG CCC^GGG
SmlI 175 CTCAAG C^T[CT][GA]AG
StuI 26 AGGCCT AGG^CCT
StyI 134 CCAAGG C^C[AT][AT]GG
TaqI 311 TCGA T^CGA
Tth111I 90 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 311 TCGA T^CGA
XcmI 293 CCAGCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 386 CCCGGG C^CCGGG
XmaI 386 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 390 GGGCCC GGGCC^C
412 GGGCCC
BanI 61 GGCACC G^G[CT][GA]CC
378 GGCGCC
BpmI 235 CTGGAG CTGGAG(16/14)
304 CTGGAG
BsrFI 164 GCCGGC [GA]^CCGG[CT]
403 GCCGGC
BstF5I 75 GGATG GGATG(2/0)
474 GGATG
BstNI 408 CCTGG CC^[AT]GG
424 CCAGG
Cfr10I 164 GCCGGC [GA]^CCGG[CT]
403 GCCGGC
DpnII 144 GATC ^GATC
482 GATC
DsaI 183 CCGCGG C^C[GA][CT]GG
374 CCGTGG
EcoRII 408 CCTGG ^CC[AT]GG
424 CCAGG
FokI 75 GGATG GGATG(9/13)
474 GGATG
MaeIII 88 GTGAC ^GT[ACGT]AC
217 GTGAC
MboI 144 GATC ^GATC
482 GATC
MvaI 408 CCTGG CC^[AT]GG
424 CCAGG
NaeI 164 GCCGGC GCC^GGC
403 GCCGGC
NdeII 144 GATC ^GATC
482 GATC
NgoMIV 164 GCCGGC G^CCGGC
403 GCCGGC
NlaIII 93 CATG CATG^
297 CATG
PstI 256 CTGCAG CTGCA^G
340 CTGCAG
PvuII 151 CAGCTG CAG^CTG
198 CAGCTG
Sau3AI 144 GATC ^GATC
482 GATC
SfcI 256 CTGCAG C^T[GA][CT]AG
340 CTGCAG
Tsp45I 88 GTGAC ^GT[GC]AC
217 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I DraII EcoO109I FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MnlI MspA1I MspI MvnI MwoI
NciI NlaIV Sau96I ScrFI TseI