Restriction map for
pFN21AB8868
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BsrI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DpnII DraI DraIII
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NcoI NdeI
NdeII NheI NotI NspI NspV
PacI PaeR7I PflFI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tsp509I TspRI
Tth111I TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AgeI 200 ACCGGT A^CCGGT
AhdI 232 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 13 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 232 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 271 GAGCAC G[AT]GC[AT]^C
BanII 254 GGGCTC G[GA]GC[CT]^C
BsaWI 200 ACCGGT [AT]^CCGG[AT]
BseRI 112 GAGGAG GAGGAG(10/8)
BsiHKAI 271 GAGCAC G[AT]GC[AT]^C
BspMI 221 ACCTGC ACCTGC(4/8)
BsrBI 363 GAGCGG CCGCTC(-3/-3)
BssHII 283 GCGCGC G^CGCGC
BstF5I 373 GGATG GGATG(2/0)
BstNI 369 CCTGG CC^[AT]GG
EaeI 119 TGGCCG [CT]^GGCC[GA]
Eam1105I 232 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 369 CCTGG ^CC[AT]GG
EcoT14I 320 CCAAGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
FokI 373 GGATG GGATG(9/13)
HinfI 350 GAATC G^A[ACGT]TC
MboII 150 GAAGA GAAGA(8/7)
MspA1I 13 CAGCTG C[AC]G^C[GT]G
MvaI 369 CCTGG CC^[AT]GG
NaeI 105 GCCGGC GCC^GGC
NgoMIV 105 GCCGGC G^CCGGC
NlaIII 360 CATG CATG^
NruI 205 TCGCGA TCG^CGA
NsiI 1 ATGCAT ATGCA^T
PflMI 12 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 200 ACCGGT A^CCGGT
PvuII 13 CAGCTG CAG^CTG
SfaNI 3 GCATC GCATC(5/9)
StyI 320 CCAAGG C^C[AT][AT]GG
TfiI 350 GAATC G^A[AT]TC
Van91I 12 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 359 CCATGAGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 78 GGCGCC G[GA]^CG[CT]C
181 GGCGCC
AvaI 73 CCCGGG C^[CT]CG[GA]G
169 CCCGGG
BanI 78 GGCGCC G^G[CT][GA]CC
181 GGCGCC
BbeI 78 GGCGCC GGCGC^C
181 GGCGCC
BmyI 254 GGGCTC G[AGT]GC[ACT]^C
271 GAGCAC
BsaHI 78 GGCGCC G[GA]^CG[CT]C
181 GGCGCC
BsiEI 198 CGACCG CG[GA][CT]^CG
202 CGGTCG
BsmFI 218 GGGAC GGGAC(10/14)
91 GTCCC
BsoBI 73 CCCGGG C^[CT]CG[GA]G
169 CCCGGG
Bsp1286I 254 GGGCTC G[AGT]GC[ACT]^C
271 GAGCAC
BsrFI 105 GCCGGC [GA]^CCGG[CT]
200 ACCGGT
Cfr10I 105 GCCGGC [GA]^CCGG[CT]
200 ACCGGT
DraII 89 AGGTCCC [GA]G^G[ACGT]CC[CT]
218 GGGACCT
EcoO109I 89 AGGTCCC [GA]G^G[ACGT]CC[CT]
218 GGGACCT
HaeII 78 GGCGCC [GA]GCGC^[CT]
181 GGCGCC
Hin1I 78 GGCGCC G[GA]^CG[CT]C
181 GGCGCC
KasI 78 GGCGCC G^GCGCC
181 GGCGCC
NarI 78 GGCGCC GG^CGCC
181 GGCGCC
PpuMI 89 AGGTCCC [GA]G^G[AT]CC[CT]
218 GGGACCT
SfoI 78 GGCGCC GGC^GCC
181 GGCGCC
SmaI 73 CCCGGG CCC^GGG
169 CCCGGG
SmlI 190 CTCAAG C^T[CT][GA]AG
337 CTCAAG
XmaCI 73 CCCGGG C^CCGGG
169 CCCGGG
XmaI 73 CCCGGG C^CCGGG
169 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BsaJI BsiYI BslI BssKI
BstUI Cac8I CfoI Cfr13I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI