(Circular) (Six-base) MAPSORT of: pFN21AB8868.seq Check: 8933 from: 1 to: 5083 pFN21AB8868 5083 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2893 278 Size: 53 83 186 936 1357 2468 Fragments arranged by size: 2468 1357 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2426 1937 Size: 489 4594 Acc65I G'GTAC_C Cuts at: 2404 2404 Size: 5083 AclI AA'CG_TT Cuts at: 1563 3210 3583 1563 Size: 1647 373 3063 Fragments arranged by size: 3063 1647 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3146 4530 4798 1632 Size: 1514 1384 268 1917 Fragments arranged by size: 1917 1514 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4886 AflIII A'CryG_T Cuts at: 1203 3982 4959 1203 Size: 2779 977 1327 Fragments arranged by size: 2779 1327 977 AgeI A'CCGG_T Cuts at: 2205 3914 2205 Size: 1709 3374 AhdI GACnn_n'nnGTC Cuts at: 2242 3812 2242 Size: 1570 3513 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5083 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5083 AlwNI CAG_nnn'CTG Cuts at: 2023 2826 4398 2023 Size: 803 1572 2708 Fragments arranged by size: 2708 1572 803 ApaLI G'TGCA_C Cuts at: 3140 4296 3140 Size: 1156 3927 ApoI r'AATT_y Cuts at: 1493 2392 2618 2770 4879 1493 Size: 899 226 152 2109 1697 Fragments arranged by size: 2109 1697 899 226 152 AseI AT'TA_AT Cuts at: 160 1032 3637 160 Size: 872 2605 1606 Fragments arranged by size: 2605 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5083 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2174 2408 1870 Size: 73 135 96 234 4545 Fragments arranged by size: 4545 234 135 96 73 BamHI G'GATC_C Cuts at: 2413 2780 2413 Size: 367 4716 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2186 2404 3859 618 Size: 325 217 627 296 103 218 1455 1842 Fragments arranged by size: 1842 1455 627 325 296 218 217 103 BanII G_rGCy'C Cuts at: 1391 2263 2402 1391 Size: 872 139 4072 Fragments arranged by size: 4072 872 139 BbeI G_GCGC'C Cuts at: 2087 2190 2087 Size: 103 4980 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4112 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3274 3308 1903 Size: 34 50 34 1253 34 3678 Fragments arranged by size: 3678 1253 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2975 4185 2975 Size: 1210 3873 BclI T'GATC_A Cuts at: 1548 1548 Size: 5083 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5083 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3694 136 Size: 107 122 71 631 605 2022 1525 Fragments arranged by size: 2022 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5078 5078 Size: 5083 BlpI GC'TnA_GC Cuts at: 2500 2500 Size: 5083 Bme1580I G_kGCm'C Cuts at: 1790 3144 4300 1790 Size: 1354 1156 2573 Fragments arranged by size: 2573 1354 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2814 3772 442 Size: 416 53 83 399 24 1397 958 1753 Fragments arranged by size: 1753 1397 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5083 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3743 1239 Size: 2504 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2180 2327 3208 4073 4371 2180 Size: 147 881 865 298 2892 Fragments arranged by size: 2892 881 865 298 147 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3746 882 Size: 617 2247 2219 Fragments arranged by size: 2247 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2874 493 Size: 711 1670 2702 Fragments arranged by size: 2702 1670 711 BsaBI GATnn'nnATC Cuts at: 5077 5077 Size: 5083 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 2187 2890 3272 Size: 53 83 186 936 551 103 703 382 Cuts at: 3272 3907 275 Size: 635 1451 Fragments arranged by size: 1451 936 703 635 551 382 186 103 83 53 BsaWI w'CCGG_w Cuts at: 2205 3516 3914 4188 4335 4746 2205 Size: 1311 398 274 147 411 2542 Fragments arranged by size: 2542 1311 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4858 Fragments arranged by size: 4858 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 1186 Size: 946 4137 BseYI C'CCAG_C Cuts at: 2016 4286 4782 2016 Size: 2270 496 2317 Fragments arranged by size: 2317 2270 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4510 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2206 2210 2533 3294 4322 1540 Size: 275 186 205 4 323 761 1028 2301 Fragments arranged by size: 2301 1028 761 323 275 205 186 4 BsiHKAI G_wGCw'C Cuts at: 2280 3144 3229 4300 2280 Size: 864 85 1071 3063 Fragments arranged by size: 3063 1071 864 85 BsmI GAATG_Cn' Cuts at: 2594 2687 2594 Size: 93 4990 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2280 2402 3144 3229 4300 1391 Size: 399 473 17 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 473 399 122 85 17 BspEI T'CCGG_A Cuts at: 4746 4746 Size: 5083 BspHI T'CATG_A Cuts at: 2972 4702 2972 Size: 1730 3353 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2235 2438 844 Size: 1391 203 3489 Fragments arranged by size: 3489 1391 203 BsrBI CCG'CTC Cuts at: 1394 1823 2370 2970 1394 Size: 429 547 600 3507 Fragments arranged by size: 3507 600 547 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3578 3752 65 Size: 1183 2330 174 1396 Fragments arranged by size: 2330 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2110 2205 3727 3914 1700 Size: 110 300 95 1522 187 2869 Fragments arranged by size: 2869 1522 300 187 110 95 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5083 BssHII G'CGCG_C Cuts at: 1925 2288 1925 Size: 363 4720 BssSI C'ACGA_G Cuts at: 3143 4155 3143 Size: 1012 4071 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5083 BstBI TT'CG_AA Cuts at: 3887 3887 Size: 5083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5083 BstYI r'GATC_y Cuts at: 1972 2413 2780 3165 3182 4634 4720 4732 5078 Size: 441 367 385 17 1452 86 12 346 Cuts at: 5078 1972 Size: 1977 Fragments arranged by size: 1977 1452 441 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4531 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4344 Fragments arranged by size: 4344 683 56 BtsI GCAGTG_nn' Cuts at: 2599 3393 3413 2599 Size: 794 20 4269 Fragments arranged by size: 4269 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5083 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5048 DraI TTT'AAA Cuts at: 2387 2387 Size: 5083 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4090 809 Size: 507 2774 1802 Fragments arranged by size: 2774 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2530 3419 Size: 54 1425 50 275 18 294 406 889 Cuts at: 3419 5048 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 406 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2530 1537 Size: 275 718 4090 Fragments arranged by size: 4090 718 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3013 1353 Size: 1660 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3670 4044 4190 387 Size: 1064 138 2081 374 146 1280 Fragments arranged by size: 2081 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3146 3743 4530 4798 1239 Size: 393 1514 597 787 268 1524 Fragments arranged by size: 1524 1514 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2095 2224 2095 Size: 129 4954 EcoRI G'AATT_C Cuts at: 2392 2770 2392 Size: 378 4705 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2335 2350 2335 Size: 15 5068 FspI TGC'GCA Cuts at: 3589 3589 Size: 5083 HaeII r_GCGC'y Cuts at: 1678 2087 2190 4230 1678 Size: 409 103 2040 2531 Fragments arranged by size: 2531 2040 409 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 3724 3756 3798 Size: 32 135 32 656 32 1464 32 42 Cuts at: 3798 3830 1373 Size: 32 2626 Fragments arranged by size: 2626 1464 656 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2427 2673 669 Size: 988 281 489 246 3079 Fragments arranged by size: 3079 988 489 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5083 HpaI GTT'AAC Cuts at: 2673 2673 Size: 5083 KasI G'GCGC_C Cuts at: 2083 2186 2083 Size: 103 4980 KpnI G_GTAC'C Cuts at: 2408 2408 Size: 5083 MfeI C'AATT_G Cuts at: 2682 2682 Size: 5083 MluI A'CGCG_T Cuts at: 4959 4959 Size: 5083 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4197 4381 1305 Size: 2892 184 2007 Fragments arranged by size: 2892 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3261 Fragments arranged by size: 3261 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3041 3400 3559 518 Size: 648 1875 359 159 2042 Fragments arranged by size: 2042 1875 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2499 3176 4324 4569 1606 Size: 414 479 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 479 414 245 NaeI GCC'GGC Cuts at: 2112 2112 Size: 5083 NarI GG'CG_CC Cuts at: 2084 2187 2084 Size: 103 4980 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4531 NdeI CA'TA_TG Cuts at: 387 387 Size: 5083 NgoMIV G'CCGG_C Cuts at: 2110 2110 Size: 5083 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5083 NotI GC'GGCC_GC Cuts at: 2530 2530 Size: 5083 NruI TCG'CGA Cuts at: 2212 2212 Size: 5083 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5083 NspI r_CATG'y Cuts at: 1132 1229 2441 3986 1132 Size: 97 1212 1545 2229 Fragments arranged by size: 2229 1545 1212 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5083 PciI A'CATG_T Cuts at: 3982 3982 Size: 5083 PflMI CCAn_nnn'nTGG Cuts at: 1108 2023 1108 Size: 915 4168 PfoI T'CCnGG_A Cuts at: 2220 2220 Size: 5083 PmeI GTTT'AAAC Cuts at: 2387 2387 Size: 5083 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3184 4665 4697 3152 Size: 32 1481 32 3538 Fragments arranged by size: 3538 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2095 2224 2095 Size: 129 4954 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5083 PsiI TTA'TAA Cuts at: 2653 2653 Size: 5083 PstI C_TGCA'G Cuts at: 830 2435 830 Size: 1605 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5083 PvuII CAG'CTG Cuts at: 2020 2020 Size: 5083 SalI G'TCGA_C Cuts at: 1936 2425 1936 Size: 489 4594 SbfI CC_TGCA'GG Cuts at: 2435 2435 Size: 5083 ScaI AGT'ACT Cuts at: 3331 5043 3331 Size: 1712 3371 SfcI C'TryA_G Cuts at: 826 1045 2431 3566 4247 4438 826 Size: 219 1386 1135 681 191 1471 Fragments arranged by size: 1471 1386 1135 681 219 191 SfoI GGC'GCC Cuts at: 2085 2188 2085 Size: 103 4980 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5083 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5083 SmaI CCC'GGG Cuts at: 1872 2080 2176 2410 1872 Size: 208 96 234 4545 Fragments arranged by size: 4545 234 208 96 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 2342 3187 4088 4350 820 Size: 197 926 252 147 845 901 262 1553 Fragments arranged by size: 1553 926 901 845 262 252 197 147 SnaBI TAC'GTA Cuts at: 493 2874 493 Size: 2381 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5083 SphI G_CATG'C Cuts at: 1132 2441 1132 Size: 1309 3774 SspI AAT'ATT Cuts at: 5 52 3007 5 Size: 47 2955 2081 Fragments arranged by size: 2955 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5083 StyI C'CwwG_G Cuts at: 513 1065 2325 2522 513 Size: 552 1260 197 3074 Fragments arranged by size: 3074 1260 552 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2367 3110 3127 3280 3465 3910 325 Size: 1255 787 743 17 153 185 445 1498 Fragments arranged by size: 1498 1255 787 743 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3329 5041 96 Size: 274 80 33 51 1443 1352 1712 138 Fragments arranged by size: 1712 1443 1352 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5043 5075 5043 Size: 32 5051 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5083 XbaI T'CTAG_A Cuts at: 2419 2419 Size: 5083 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2371 2371 Size: 5083 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5083 XmaI C'CCGG_G Cuts at: 1870 2078 2174 2408 1870 Size: 208 96 234 4545 Fragments arranged by size: 4545 234 208 96 XmnI GAAnn'nnTTC Cuts at: 3212 3212 Size: 5083 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2891 276 Size: 53 83 186 936 1357 2468 Fragments arranged by size: 2468 1357 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI