Restriction map for
pFN21AB8866
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI HpaII HphI KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp45I
TspRI Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Alw44I 184 GTGCAC G^TGCAC
ApaLI 184 GTGCAC G^TGCAC
AspI 259 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 206 GAGCTC G[GA]GC[CT]^C
BbsI 256 GAAGAC GAAGAC(2/6)
BfaI 214 CTAG C^TAG
BglII 91 AGATCT A^GATCT
BpuAI 256 GAAGAC GAAGAC(2/6)
BseRI 209 CTCCTC GAGGAG(10/8)
BsiYI 246 CCGAAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 246 CCGAAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 6 ACCTGC ACCTGC(4/8)
BssKI 123 CCTGG ^CC[ACGT]GG
BstAPI 177 GCAGGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 123 CCTGG CC^[AT]GG
BstYI 91 AGATCT [GA]^GATC[CT]
Cfr13I 242 GGCCC G^G[ACGT]CC
DpnII 92 GATC ^GATC
DraII 241 AGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 87 GAAGAG CTCTTC(1/4)
EcoO109I 241 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 123 CCTGG ^CC[AT]GG
EcoT14I 201 CCTTGG C^C[AT][AT]GG
EcoT22I 115 ATGCAT ATGCA^T
HaeIII 242 GGCC GG^CC
Ksp632I 87 GAAGAG CTCTTC(1/4)
MaeI 214 CTAG C^TAG
MboI 92 GATC ^GATC
MflI 91 AGATCT [GA]^GATC[CT]
MvaI 123 CCTGG CC^[AT]GG
NdeII 92 GATC ^GATC
NsiI 115 ATGCAT ATGCA^T
PflFI 259 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 175 CTGCAG CTGCA^G
SacI 206 GAGCTC GAGCT^C
Sau3AI 92 GATC ^GATC
Sau96I 242 GGCCC G^G[ACGT]CC
ScrFI 123 CCTGG CC^[ACGT]GG
SfaNI 226 GATGC GCATC(5/9)
SfcI 175 CTGCAG C^T[GA][CT]AG
StyI 201 CCTTGG C^C[AT][AT]GG
TaqI 142 TCGA T^CGA
Tth111I 259 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 142 TCGA T^CGA
XhoII 91 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AspHI 184 GTGCAC G[AT]GC[AT]^C
206 GAGCTC
BbvI 162 GCTGC GCAGC(8/12)
174 GCTGC
BmyI 184 GTGCAC G[AGT]GC[ACT]^C
206 GAGCTC
BsaJI 123 CCTGGG C^C[ACGT][ACGT]GG
201 CCTTGG
BsiHKAI 184 GTGCAC G[AT]GC[AT]^C
206 GAGCTC
Bsp1286I 184 GTGCAC G[AGT]GC[ACT]^C
206 GAGCTC
BstF5I 69 GGATG GGATG(2/0)
180 GGATG
Fnu4HI 162 GCTGC GC^[ACGT]GC
174 GCTGC
FokI 69 GGATG GGATG(9/13)
180 GGATG
ItaI 162 GCTGC GC^[ACGT]GC
174 GCTGC
MboII 87 GAAGA GAAGA(8/7)
256 GAAGA
MseI 83 TTAA T^TAA
325 TTAA
NlaIV 232 GGTTCC GG[ACGT]^[ACGT]CC
242 GGCCCC
Tru9I 83 TTAA T^TAA
325 TTAA
TseI 162 GCTGC G^C[AT]GC
174 GCTGC
Tsp509I 48 AATT ^AATT
190 AATT
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
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AluI MnlI MwoI NlaIII