Restriction map for
pFN21AB8861
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstNI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRII EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeII
MfeI MflI MluI MroI MseI
MslI MspA1I MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIII NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScrFI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AfaI 225 GTAC GT^AC
AspHI 208 GAGCTC G[AT]GC[AT]^C
BalI 248 TGGCCA TGG^CCA
BbvCI 96 CCTCAGC CCTCAGC(-5/-2)
BbvI 4 GCAGC GCAGC(8/12)
BfaI 25 CTAG C^TAG
BpmI 228 CTGGAG CTGGAG(16/14)
BsaBI 190 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 40 GAGACC GGTCTC(1/5)
BseRI 28 GAGGAG GAGGAG(10/8)
BsiHKAI 208 GAGCTC G[AT]GC[AT]^C
BsiYI 65 CCTTTGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 65 CCTTTGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 40 GAGAC GTCTC(1/5)
BsrI 227 ACTGG ACTGG(1/-1)
Cac8I 246 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 81 GGGCC G^G[ACGT]CC
DdeI 97 CTCAG C^T[ACGT]AG
DpnII 282 GATC ^GATC
DsaI 44 CCGTGG C^C[GA][CT]GG
EaeI 248 TGGCCA [CT]^GGCC[GA]
EarI 180 GAAGAG CTCTTC(1/4)
EcoNI 65 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 251 CCAAGG C^C[AT][AT]GG
Fnu4HI 4 GCAGC GC^[ACGT]GC
HapII 84 CCGG C^CGG
HgaI 101 GCGTC GACGC(5/10)
HincII 103 GTCAAC GT[CT]^[GA]AC
HindII 103 GTCAAC GT[CT]^[GA]AC
HpaII 84 CCGG C^CGG
ItaI 4 GCAGC GC^[ACGT]GC
Ksp632I 180 GAAGAG CTCTTC(1/4)
MaeI 25 CTAG C^TAG
MamI 190 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 282 GATC ^GATC
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
MspI 84 CCGG C^CGG
MwoI 138 GCCCCATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 282 GATC ^GATC
PshAI 42 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 225 GTAC GT^AC
SacI 208 GAGCTC GAGCT^C
SapI 180 GAAGAGC GCTCTTC(1/4)
Sau3AI 282 GATC ^GATC
Sau96I 81 GGGCC G^G[ACGT]CC
ScaI 224 AGTACT AGT^ACT
StyI 251 CCAAGG C^C[AT][AT]GG
TfiI 195 GAATC G^A[AT]TC
TseI 4 GCAGC G^C[AT]GC
Tsp45I 49 GTCAC ^GT[GC]AC
TspRI 14 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 35 CCATTGAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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BanII 159 GGGCTC G[GA]GC[CT]^C
208 GAGCTC
BmyI 159 GGGCTC G[AGT]GC[ACT]^C
208 GAGCTC
BsaJI 44 CCGTGG C^C[ACGT][ACGT]GG
251 CCAAGG
Bsp1286I 159 GGGCTC G[AGT]GC[ACT]^C
208 GAGCTC
BstEII 48 GGTCACC G^GT[ACGT]ACC
123 GGTTACC
BstF5I 153 GGATG GGATG(2/0)
189 GGATG
BstPI 48 GGTCACC G^GT[ACGT]ACC
123 GGTTACC
EcoO65I 48 GGTCACC G^GT[ACGT]ACC
123 GGTTACC
FokI 153 GGATG GGATG(9/13)
189 GGATG
HaeIII 82 GGCC GG^CC
249 GGCC
HphI 50 TCACC GGTGA(8/7)
62 TCACC
MaeIII 49 GTCAC ^GT[ACGT]AC
124 GTTAC
PleI 202 GACTC GAGTC(4/5)
31 GAGTC
SmlI 148 CTCAAG C^T[CT][GA]AG
211 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
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AluI HinfI MboII MnlI