(Linear) (Six-base) MAPSORT of: KIBB8861.seq  Check: 9399  from: 1  to: 294



KIBB8861.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     199     231     294 
   Size:        199      32      63

  Fragments arranged by size: 

                 199      63      32
 
BanII G_rGCy'C

Cuts at:      0     163     212     294 
   Size:        163      49      82

  Fragments arranged by size: 

                 163      82      49
 
BbvCI CC'TCA_GC

Cuts at:      0      97     294 
   Size:         97     197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     294 
   Size:        249      45
 
Bpu10I CC'TnA_GC

Cuts at:      0      97     294 
   Size:         97     197
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     196     294 
   Size:        133      63      98

  Fragments arranged by size: 

                 133      98      63
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      34     294 
   Size:         34     260
 
BsaBI GATnn'nnATC

Cuts at:      0     194     294 
   Size:        194     100
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     199     229     294 
   Size:        199      30      65

  Fragments arranged by size: 

                 199      65      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     294 
   Size:         43     251
 
BseYI C'CCAG_C

Cuts at:      0     128     294 
   Size:        128     166
 
BsiHKAI G_wGCw'C

Cuts at:      0     212     294 
   Size:        212      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     163     212     294 
   Size:        163      49      82

  Fragments arranged by size: 

                 163      82      49
 
BstEII G'GTnAC_C

Cuts at:      0      48     123     294 
   Size:         48      75     171

  Fragments arranged by size: 

                 171      75      48
 
BtgI C'CryG_G

Cuts at:      0      44     294 
   Size:         44     250
 
EaeI y'GGCC_r

Cuts at:      0     248     294 
   Size:        248      46
 
EarI CTCTTCn'nnn_

Cuts at:      0     175     294 
   Size:        175     119
 
EcoICRI GAG'CTC

Cuts at:      0     210     294 
   Size:        210      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     294 
   Size:        249      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      69     294 
   Size:         69     225
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     145     177     294 
   Size:        145      32     117

  Fragments arranged by size: 

                 145     117      32
 
HincII GTy'rAC

Cuts at:      0     105     294 
   Size:        105     189
 
MscI TGG'CCA

Cuts at:      0     250     294 
   Size:        250      44
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     199     231     294 
   Size:        199      32      63

  Fragments arranged by size: 

                 199      63      32
 
PshAI GACnn'nnGTC

Cuts at:      0      46     294 
   Size:         46     248
 
SacI G_AGCT'C

Cuts at:      0     212     294 
   Size:        212      82
 
SapI GCTCTTCn'nnn_

Cuts at:      0     175     294 
   Size:        175     119
 
ScaI AGT'ACT

Cuts at:      0     226     294 
   Size:        226      68
 
SmlI C'TyrA_G

Cuts at:      0     148     211     294 
   Size:        148      63      83

  Fragments arranged by size: 

                 148      83      63
 
StyI C'CwwG_G

Cuts at:      0     251     294 
   Size:        251      43
 
TatI w'GTAC_w

Cuts at:      0     224     294 
   Size:        224      70
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     294 
   Size:         42     252
 

 Enzymes that do cut:

     AloI    BanII    BbvCI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I   BstEII     BtgI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI    Hin4I   HincII     MscI     PpiI
    PshAI     SacI     SapI     ScaI     SmlI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BsaAI
    BsaHI    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI