(Circular) (Six-base) MAPSORT of: pFN21AB8848.seq Check: 3251 from: 1 to: 4906 pFN21AB8848 4906 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2716 278 Size: 53 83 186 936 1180 2468 Fragments arranged by size: 2468 1180 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2249 1937 Size: 312 4594 Acc65I G'GTAC_C Cuts at: 2227 2227 Size: 4906 AclI AA'CG_TT Cuts at: 1563 3033 3406 1563 Size: 1470 373 3063 Fragments arranged by size: 3063 1470 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2969 4353 4621 1632 Size: 512 825 1384 268 1917 Fragments arranged by size: 1917 1384 825 512 268 AfeI AGC'GCT Cuts at: 2129 2129 Size: 4906 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4709 AflIII A'CryG_T Cuts at: 1203 3805 4782 1203 Size: 2602 977 1327 Fragments arranged by size: 2602 1327 977 AgeI A'CCGG_T Cuts at: 3737 3737 Size: 4906 AhdI GACnn_n'nnGTC Cuts at: 3635 3635 Size: 4906 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4906 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4906 AlwNI CAG_nnn'CTG Cuts at: 2649 4221 2649 Size: 1572 3334 ApaLI G'TGCA_C Cuts at: 2963 4119 2963 Size: 1156 3750 ApoI r'AATT_y Cuts at: 1493 2215 2441 2593 4702 1493 Size: 722 226 152 2109 1697 Fragments arranged by size: 2109 1697 722 226 152 AseI AT'TA_AT Cuts at: 160 1032 3460 160 Size: 872 2428 1606 Fragments arranged by size: 2428 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4906 AvaI C'yCGr_G Cuts at: 1870 1943 2231 1870 Size: 73 288 4545 Fragments arranged by size: 4545 288 73 BamHI G'GATC_C Cuts at: 2236 2603 2236 Size: 367 4539 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 3682 618 Size: 325 217 627 440 1455 1842 Fragments arranged by size: 1842 1455 627 440 325 217 BanII G_rGCy'C Cuts at: 1391 2225 1391 Size: 834 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3935 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2026 2060 2084 2118 3097 3131 1903 Size: 34 89 34 24 34 979 34 3678 Fragments arranged by size: 3678 979 89 34 34 34 34 24 BciVI GTATCCnnnnn_n' Cuts at: 2798 4008 2798 Size: 1210 3696 BclI T'GATC_A Cuts at: 1548 2007 1548 Size: 459 4447 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3517 136 Size: 107 122 71 631 605 1845 1525 Fragments arranged by size: 1845 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4901 4901 Size: 4906 BlpI GC'TnA_GC Cuts at: 2323 2323 Size: 4906 Bme1580I G_kGCm'C Cuts at: 1790 2967 4123 1790 Size: 1177 1156 2573 Fragments arranged by size: 2573 1177 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4906 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2637 3595 442 Size: 416 53 83 399 24 1220 958 1753 Fragments arranged by size: 1753 1220 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4906 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 3566 1239 Size: 980 1347 2579 Fragments arranged by size: 2579 1347 980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3031 3896 4194 3031 Size: 865 298 3743 Fragments arranged by size: 3743 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3569 882 Size: 617 2070 2219 Fragments arranged by size: 2219 2070 617 BsaAI yAC'GTr Cuts at: 493 1204 2161 2697 493 Size: 711 957 536 2702 Fragments arranged by size: 2702 957 711 536 BsaBI GATnn'nnATC Cuts at: 4900 4900 Size: 4906 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2713 3095 3730 275 Size: 53 83 186 936 1180 382 635 1451 Fragments arranged by size: 1451 1180 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3339 3737 4011 4158 4569 3339 Size: 398 274 147 411 3676 Fragments arranged by size: 3676 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4681 Fragments arranged by size: 4681 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4906 BseYI C'CCAG_C Cuts at: 4109 4605 4109 Size: 496 4410 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2356 3117 4145 1540 Size: 275 186 355 761 1028 2301 Fragments arranged by size: 2301 1028 761 355 275 186 BsiHKAI G_wGCw'C Cuts at: 2967 3052 4123 2967 Size: 85 1071 3750 Fragments arranged by size: 3750 1071 85 BsmI GAATG_Cn' Cuts at: 2417 2510 2417 Size: 93 4813 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2225 2967 3052 4123 1391 Size: 399 435 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 85 BspEI T'CCGG_A Cuts at: 4569 4569 Size: 4906 BspHI T'CATG_A Cuts at: 2795 4525 2795 Size: 1730 3176 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2261 844 Size: 1417 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2793 1394 Size: 429 970 3507 Fragments arranged by size: 3507 970 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3401 3575 65 Size: 1183 2153 174 1396 Fragments arranged by size: 2153 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3550 3737 1700 Size: 110 1740 187 2869 Fragments arranged by size: 2869 1740 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4906 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4906 BssSI C'ACGA_G Cuts at: 2966 3978 2966 Size: 1012 3894 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4906 BstBI TT'CG_AA Cuts at: 3710 3710 Size: 4906 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4906 BstYI r'GATC_y Cuts at: 1972 2236 2603 2988 3005 4457 4543 4555 4901 Size: 264 367 385 17 1452 86 12 346 Cuts at: 4901 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 264 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4354 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4167 Fragments arranged by size: 4167 683 56 BtsI GCAGTG_nn' Cuts at: 2422 3216 3236 2422 Size: 794 20 4092 Fragments arranged by size: 4092 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4906 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4871 DraI TTT'AAA Cuts at: 2210 2210 Size: 4906 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3913 809 Size: 507 2597 1802 Fragments arranged by size: 2597 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2353 3242 4871 Size: 54 1425 50 275 18 523 889 1629 Cuts at: 4871 8 Size: 43 Fragments arranged by size: 1629 1425 889 523 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2353 1537 Size: 275 541 4090 Fragments arranged by size: 4090 541 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2836 1353 Size: 1483 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3493 3867 4013 387 Size: 1064 138 1904 374 146 1280 Fragments arranged by size: 1904 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2219 2969 3566 4353 4621 1239 Size: 393 512 75 750 597 787 268 1524 Fragments arranged by size: 1524 787 750 597 512 393 268 75 EcoNI CCTnn'n_nnAGG Cuts at: 2201 2201 Size: 4906 EcoRI G'AATT_C Cuts at: 2215 2593 2215 Size: 378 4528 FspI TGC'GCA Cuts at: 3412 3412 Size: 4906 HaeII r_GCGC'y Cuts at: 1678 2131 4053 1678 Size: 453 1922 2531 Fragments arranged by size: 2531 1922 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3547 3579 3621 3653 1373 Size: 32 135 32 1975 32 42 32 2626 Fragments arranged by size: 2626 1975 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2250 2496 669 Size: 988 281 312 246 3079 Fragments arranged by size: 3079 988 312 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4906 HpaI GTT'AAC Cuts at: 2496 2496 Size: 4906 KpnI G_GTAC'C Cuts at: 2231 2231 Size: 4906 MfeI C'AATT_G Cuts at: 2505 2505 Size: 4906 MluI A'CGCG_T Cuts at: 4782 4782 Size: 4906 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4020 4204 1305 Size: 2715 184 2007 Fragments arranged by size: 2715 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3084 Fragments arranged by size: 3084 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2864 3223 3382 518 Size: 648 1698 359 159 2042 Fragments arranged by size: 2042 1698 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2322 2999 4147 4392 1606 Size: 716 677 1148 245 2120 Fragments arranged by size: 2120 1148 716 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4354 NdeI CA'TA_TG Cuts at: 387 387 Size: 4906 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4906 NotI GC'GGCC_GC Cuts at: 2353 2353 Size: 4906 NspI r_CATG'y Cuts at: 1132 1229 2264 3809 1132 Size: 97 1035 1545 2229 Fragments arranged by size: 2229 1545 1035 97 PasI CC'CwG_GG Cuts at: 1348 2116 1348 Size: 768 4138 PciI A'CATG_T Cuts at: 3805 3805 Size: 4906 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4906 PmeI GTTT'AAAC Cuts at: 2210 2210 Size: 4906 PmlI CAC'GTG Cuts at: 2161 2161 Size: 4906 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2975 3007 4488 4520 2975 Size: 32 1481 32 3361 Fragments arranged by size: 3361 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4906 PsiI TTA'TAA Cuts at: 2476 2476 Size: 4906 PstI C_TGCA'G Cuts at: 830 2082 2258 830 Size: 1252 176 3478 Fragments arranged by size: 3478 1252 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4906 SalI G'TCGA_C Cuts at: 1936 2248 1936 Size: 312 4594 SbfI CC_TGCA'GG Cuts at: 2258 2258 Size: 4906 ScaI AGT'ACT Cuts at: 3154 4866 3154 Size: 1712 3194 SfcI C'TryA_G Cuts at: 826 1045 2078 2254 3389 4070 4261 826 Size: 219 1033 176 1135 681 191 1471 Fragments arranged by size: 1471 1135 1033 681 219 191 176 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4906 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4906 SmaI CCC'GGG Cuts at: 1872 2233 1872 Size: 361 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3010 3911 4173 820 Size: 197 926 1067 901 262 1553 Fragments arranged by size: 1553 1067 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2697 493 Size: 2204 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4906 SphI G_CATG'C Cuts at: 1132 2264 1132 Size: 1132 3774 SspI AAT'ATT Cuts at: 5 52 2830 5 Size: 47 2778 2081 Fragments arranged by size: 2778 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4906 StyI C'CwwG_G Cuts at: 513 1065 2345 513 Size: 552 1280 3074 Fragments arranged by size: 3074 1280 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2933 2950 3103 3288 3733 325 Size: 1255 1353 17 153 185 445 1498 Fragments arranged by size: 1498 1353 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 3152 4864 Size: 274 80 33 51 1443 111 1064 1712 Cuts at: 4864 96 Size: 138 Fragments arranged by size: 1712 1443 1064 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4866 4898 4866 Size: 32 4874 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4906 XbaI T'CTAG_A Cuts at: 2242 2242 Size: 4906 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4906 XmaI C'CCGG_G Cuts at: 1870 2231 1870 Size: 361 4545 XmnI GAAnn'nnTTC Cuts at: 3035 3035 Size: 4906 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2714 276 Size: 53 83 186 936 1180 2468 Fragments arranged by size: 2468 1180 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI