Restriction map for
pFN21AB8842
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsaHI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI BstBI BstUI Bsu36I
CfoI ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII KasI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NciI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I Van91I XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 808 GGTACC G^GTACC
Asp718I 808 GGTACC G^GTACC
BamHI 441 GGATCC G^GATCC
BanI 808 GGTACC G^G[CT][GA]CC
BanII 109 GGGCTC G[GA]GC[CT]^C
BglII 230 AGATCT A^GATCT
BlpI 608 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 608 GCTCAGC GC^T[ACGT]AGC
BsaI 1303 GAGACC GGTCTC(1/5)
BsaWI 245 ACCGGA [AT]^CCGG[AT]
BsiEI 708 CGACCG CG[GA][CT]^CG
BsmFI 552 GTCCC GGGAC(10/14)
BsrBI 1387 GAGCGG CCGCTC(-3/-3)
BsrFI 1239 GCCGGC [GA]^CCGG[CT]
Bst1107I 639 GTATAC GTA^TAC
BstEII 777 GGTCACC G^GT[ACGT]ACC
BstPI 777 GGTCACC G^GT[ACGT]ACC
BstZ17I 639 GTATAC GTA^TAC
CelII 608 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1239 GCCGGC [GA]^CCGG[CT]
DraIII 600 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1415 TGGCCG [CT]^GGCC[GA]
EcoO65I 777 GGTCACC G^GT[ACGT]ACC
HincII 154 GTTAAC GT[CT]^[GA]AC
HindII 154 GTTAAC GT[CT]^[GA]AC
HpaI 154 GTTAAC GTT^AAC
KpnI 808 GGTACC GGTAC^C
MboII 1105 GAAGA GAAGA(8/7)
MslI 432 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1239 GCCGGC GCC^GGC
NcoI 86 CCATGG C^CATGG
NgoMIV 1239 GCCGGC G^CCGGC
PaeR7I 772 CTCGAG C^TCGAG
PpuMI 494 AGGTCCT [GA]G^G[AT]CC[CT]
SexAI 149 ACCAGGT A^CC[AT]GGT
StuI 369 AGGCCT AGG^CCT
TaqI 773 TCGA T^CGA
TfiI 967 GAATC G^A[AT]TC
TthHB8I 773 TCGA T^CGA
XbaI 1272 TCTAGA T^CTAGA
XcmI 77 CCATCTCTCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 772 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 334 GTCTAC GT^[AC][GT]AC
639 GTATAC
AfaI 428 GTAC GT^AC
809 GTAC
AlwNI 892 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1096 CAGATCCTG
BbvCI 792 CCTCAGC CCTCAGC(-5/-2)
490 GCTGAGG
BseRI 1342 GAGGAG GAGGAG(10/8)
1378 GAGGAG
BsmAI 9 GAGAC GTCTC(1/5)
1303 GAGAC
BspMI 1172 ACCTGC ACCTGC(4/8)
1443 GCAGGT
BstAPI 599 GCACAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1255 GCAGGCTGTGC
BstXI 431 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCAGTTCTTTGG
EcoNI 114 CCTGATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1335 CCTCACCGAGG
FauI 1208 CCCGC CCCGC(4/6)
1407 GCGGG
HapII 246 CCGG C^CGG
1240 CCGG
HpaII 246 CCGG C^CGG
1240 CCGG
MspI 246 CCGG C^CGG
1240 CCGG
NspI 854 ACATGC [GA]CATG^[CT]
1232 ACATGC
RsaI 428 GTAC GT^AC
809 GTAC
Tsp45I 778 GTCAC ^GT[GC]AC
1327 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI Tru9I
TseI TspRI XhoII