(Circular) (Six-base) MAPSORT of: pFN21AB8842.seq Check: 823 from: 1 to: 6160 pFN21AB8842 6160 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3186 3186 Size: 6160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2340 2645 3503 1937 Size: 403 305 858 4594 Fragments arranged by size: 4594 858 403 305 Acc65I G'GTAC_C Cuts at: 2813 3481 2813 Size: 668 5492 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2391 4223 5607 5875 1632 Size: 759 1832 1384 268 1917 Fragments arranged by size: 1917 1832 1384 759 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2889 1184 Size: 1705 4455 AlwNI CAG_nnn'CTG Cuts at: 2902 3106 3903 5475 2902 Size: 204 797 1572 3587 Fragments arranged by size: 3587 1572 797 204 ApaLI G'TGCA_C Cuts at: 4217 5373 4217 Size: 1156 5004 ApoI r'AATT_y Cuts at: 1493 3469 3695 3847 5956 1493 Size: 1976 226 152 2109 1697 Fragments arranged by size: 2109 1976 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2304 2331 2684 2777 3485 1870 Size: 73 361 27 353 93 708 4545 Fragments arranged by size: 4545 708 361 353 93 73 27 BamHI G'GATC_C Cuts at: 2446 3490 3857 2446 Size: 1044 367 4749 Fragments arranged by size: 4749 1044 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2813 3481 4936 618 Size: 325 217 627 1026 668 1455 1842 Fragments arranged by size: 1842 1455 1026 668 627 325 217 BanII G_rGCy'C Cuts at: 1391 2118 3479 1391 Size: 727 1361 4072 Fragments arranged by size: 4072 1361 727 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5189 BbvCI CC'TCA_GC Cuts at: 2496 2798 2496 Size: 302 5858 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4351 4385 1903 Size: 34 50 34 2330 34 3678 Fragments arranged by size: 3678 2330 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5262 4052 Size: 1210 4950 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4771 136 Size: 107 122 71 631 605 3099 1525 Fragments arranged by size: 3099 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2235 6155 2235 Size: 3920 2240 BlpI GC'TnA_GC Cuts at: 2614 3577 2614 Size: 963 5197 Bme1580I G_kGCm'C Cuts at: 1790 2911 3214 4221 5377 1790 Size: 1121 303 1007 1156 2573 Fragments arranged by size: 2573 1156 1121 1007 303 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2167 3891 4849 Size: 416 53 83 399 24 750 1724 958 Cuts at: 4849 442 Size: 1753 Fragments arranged by size: 1753 1724 958 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2710 2710 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2519 2655 2758 2978 3212 4820 1239 Size: 1097 183 136 103 220 234 1608 2579 Fragments arranged by size: 2579 1608 1097 234 220 183 136 103 Bpu10I CC'TnA_GC Cuts at: 2496 2654 2798 2496 Size: 158 144 5858 Fragments arranged by size: 5858 158 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2030 2116 4285 5150 5448 2030 Size: 86 2169 865 298 2742 Fragments arranged by size: 2742 2169 865 298 86 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3302 4823 882 Size: 617 1803 1521 2219 Fragments arranged by size: 2219 1803 1521 617 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3967 4349 4984 275 Size: 53 83 186 936 2434 382 635 1451 Fragments arranged by size: 2434 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2250 4593 4991 5265 5412 5823 2250 Size: 2343 398 274 147 411 2587 Fragments arranged by size: 2587 2343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5935 Fragments arranged by size: 5935 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3362 3398 1186 Size: 2176 36 3948 Fragments arranged by size: 3948 2176 36 BseYI C'CCAG_C Cuts at: 3132 5363 5859 3132 Size: 2231 496 3433 Fragments arranged by size: 3433 2231 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2330 2989 3146 3358 1163 Size: 573 399 195 659 157 212 3965 Fragments arranged by size: 3965 659 573 399 212 195 157 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2716 3610 4371 5399 1540 Size: 275 186 715 894 761 1028 2301 Fragments arranged by size: 2301 1028 894 761 715 275 186 BsiHKAI G_wGCw'C Cuts at: 2606 2615 3228 4221 4306 5377 2606 Size: 9 613 993 85 1071 3389 Fragments arranged by size: 3389 1071 993 613 85 9 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2606 2615 2911 3214 3228 3479 Size: 399 328 488 9 296 303 14 251 Cuts at: 3479 4221 4306 5377 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 488 399 328 303 296 251 85 14 9 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3186 3439 3515 844 Size: 2342 253 76 3489 Fragments arranged by size: 3489 2342 253 76 BsrBI CCG'CTC Cuts at: 1394 1823 3394 4047 1394 Size: 429 1571 653 3507 Fragments arranged by size: 3507 1571 653 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4655 4829 65 Size: 1183 3407 174 1396 Fragments arranged by size: 3407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3244 4804 4991 1700 Size: 110 1434 1560 187 2869 Fragments arranged by size: 2869 1560 1434 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6160 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 2610 3266 794 Size: 1816 656 3688 Fragments arranged by size: 3688 1816 656 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 2782 1184 Size: 1598 4562 BstXI CCAn_nnnn'nTGG Cuts at: 2443 2810 2443 Size: 367 5793 BstYI r'GATC_y Cuts at: 1972 2235 2446 3102 3490 3857 4242 4259 5711 Size: 263 211 656 388 367 385 17 1452 Cuts at: 5711 5797 5809 6155 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 656 388 385 367 346 263 211 86 17 12 BstZ17I GTA'TAC Cuts at: 2646 2646 Size: 6160 BtgI C'CryG_G Cuts at: 513 1065 2027 2091 2571 513 Size: 552 962 64 480 4102 Fragments arranged by size: 4102 962 552 480 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 2462 3336 3370 3676 4470 4490 2462 Size: 874 34 306 794 20 4132 Fragments arranged by size: 4132 874 794 306 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DraIII CAC_nnn'GTG Cuts at: 2610 2610 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3420 3607 4496 Size: 54 1425 50 275 18 1590 187 889 Cuts at: 4496 6125 8 Size: 1629 43 Fragments arranged by size: 1629 1590 1425 889 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4090 1353 Size: 2737 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4747 5121 5267 387 Size: 1064 138 3158 374 146 1280 Fragments arranged by size: 3158 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2391 2519 2655 2758 2978 3212 Size: 393 704 55 128 136 103 220 234 Cuts at: 3212 4223 4820 5607 5875 1239 Size: 1011 597 787 268 1524 Fragments arranged by size: 1524 1011 787 704 597 393 268 234 220 136 128 103 55 EcoNI CCTnn'n_nnAGG Cuts at: 2123 3344 2123 Size: 1221 4939 EcoO109I rG'GnC_Cy Cuts at: 2500 2899 3169 2500 Size: 399 270 5491 Fragments arranged by size: 5491 399 270 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 6160 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 5307 1678 Size: 3629 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4801 4833 4875 4907 1373 Size: 32 135 32 3229 32 42 32 2626 Fragments arranged by size: 3229 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 3504 3750 669 Size: 988 281 223 1343 246 3079 Fragments arranged by size: 3079 1343 988 281 246 223 HindIII A'AGCT_T Cuts at: 748 748 Size: 6160 HpaI GTT'AAC Cuts at: 2161 3750 2161 Size: 1589 4571 KpnI G_GTAC'C Cuts at: 2817 3485 2817 Size: 668 5492 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3478 5274 5458 1305 Size: 2173 1796 184 2007 Fragments arranged by size: 2173 2007 1796 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4338 Fragments arranged by size: 4338 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2441 4118 4477 4636 518 Size: 648 1275 1677 359 159 2042 Fragments arranged by size: 2042 1677 1275 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2036 2314 2495 2554 2736 2965 3147 3153 Size: 430 278 181 59 182 229 182 6 Cuts at: 3153 3361 3429 3576 4253 5401 5646 1606 Size: 208 68 147 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 430 278 245 229 208 182 182 181 147 68 59 6 NaeI GCC'GGC Cuts at: 3246 3246 Size: 6160 NcoI C'CATG_G Cuts at: 513 1065 2091 513 Size: 552 1026 4582 Fragments arranged by size: 4582 1026 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NgoMIV G'CCGG_C Cuts at: 3244 3244 Size: 6160 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 2863 3241 3518 5063 1132 Size: 97 1634 378 277 1545 2229 Fragments arranged by size: 2229 1634 1545 378 277 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6160 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PfoI T'CCnGG_A Cuts at: 3105 3105 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2500 2500 Size: 6160 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PspXI vC'TCGA_Gb Cuts at: 2777 2777 Size: 6160 PstI C_TGCA'G Cuts at: 830 2601 2878 3152 3512 830 Size: 1771 277 274 360 3478 Fragments arranged by size: 3478 1771 360 277 274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 2036 2314 2495 2554 2965 3147 3153 3361 3429 Size: 278 181 59 411 182 6 208 68 Cuts at: 3429 2036 Size: 4767 Fragments arranged by size: 4767 411 278 208 182 181 68 59 6 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SexAI A'CCwGG_T Cuts at: 2154 2154 Size: 6160 SfcI C'TryA_G Cuts at: 826 1045 2597 2874 3148 3508 4643 5324 5515 Size: 219 1552 277 274 360 1135 681 191 Cuts at: 5515 826 Size: 1471 Fragments arranged by size: 1552 1471 1135 681 360 277 274 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 3487 1872 Size: 1615 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2009 2131 2777 4264 5165 5427 Size: 197 926 66 122 646 1487 901 262 Cuts at: 5427 820 Size: 1553 Fragments arranged by size: 1553 1487 926 901 646 262 197 122 66 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 2376 1517 Size: 859 5301 StyI C'CwwG_G Cuts at: 513 1065 2062 2091 2264 2358 3378 3599 513 Size: 552 997 29 173 94 1020 221 3074 Fragments arranged by size: 3074 1020 997 552 221 173 94 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2804 4187 4204 4357 4542 4987 325 Size: 1255 1224 1383 17 153 185 445 1498 Fragments arranged by size: 1498 1383 1255 1224 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4406 6118 96 Size: 274 80 33 51 1443 2429 1712 138 Fragments arranged by size: 2429 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2292 2324 2454 2486 6120 6152 2292 Size: 32 130 32 3634 32 2300 Fragments arranged by size: 3634 2300 130 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3277 3496 3277 Size: 219 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2089 2089 Size: 6160 XhoI C'TCGA_G Cuts at: 1943 2777 1943 Size: 834 5326 XmaI C'CCGG_G Cuts at: 1870 3485 1870 Size: 1615 4545 XmnI GAAnn'nnTTC Cuts at: 4289 4289 Size: 6160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI