Restriction map for
pFN21AB8842
(Linear) (Six-base) MAPSORT of: KIBB8842.seq Check: 9146 from: 1 to: 1455
KIBB8842.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1181 1455
Size: 1181 274
AccI GT'mk_AC
Cuts at: 0 335 640 1455
Size: 335 305 815
Fragments arranged by size:
815 335 305
Acc65I G'GTAC_C
Cuts at: 0 808 1455
Size: 808 647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1455
Size: 386 1069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 884 1455
Size: 884 571
AlwNI CAG_nnn'CTG
Cuts at: 0 897 1101 1455
Size: 897 204 354
Fragments arranged by size:
897 354 204
AvaI C'yCGr_G
Cuts at: 0 299 326 679 772 1455
Size: 299 27 353 93 683
Fragments arranged by size:
683 353 299 93 27
BamHI G'GATC_C
Cuts at: 0 441 1455
Size: 441 1014
BanI G'GyrC_C
Cuts at: 0 808 1455
Size: 808 647
BanII G_rGCy'C
Cuts at: 0 113 1455
Size: 113 1342
BbvCI CC'TCA_GC
Cuts at: 0 491 793 1455
Size: 491 302 662
Fragments arranged by size:
662 491 302
BglII A'GATC_T
Cuts at: 0 230 1455
Size: 230 1225
BlpI GC'TnA_GC
Cuts at: 0 609 1455
Size: 609 846
Bme1580I G_kGCm'C
Cuts at: 0 906 1209 1455
Size: 906 303 246
Fragments arranged by size:
906 303 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 162 1455
Size: 162 1293
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 705 1455
Size: 705 750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 514 650 753 973 1207 1455
Size: 331 183 136 103 220 234 248
Fragments arranged by size:
331 248 234 220 183 136 103
Bpu10I CC'TnA_GC
Cuts at: 0 491 649 793 1455
Size: 491 158 144 662
Fragments arranged by size:
662 491 158 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 111 1455
Size: 25 86 1344
Fragments arranged by size:
1344 86 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 1297 1455
Size: 1297 158
BsaWI w'CCGG_w
Cuts at: 0 245 1455
Size: 245 1210
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1357 1393 1455
Size: 1357 36 62
Fragments arranged by size:
1357 62 36
BseYI C'CCAG_C
Cuts at: 0 1127 1455
Size: 1127 328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 325 984 1141 1353 1455
Size: 130 195 659 157 212 102
Fragments arranged by size:
659 212 195 157 130 102
BsiEI CG_ry'CG
Cuts at: 0 711 1455
Size: 711 744
BsiHKAI G_wGCw'C
Cuts at: 0 601 610 1223 1455
Size: 601 9 613 232
Fragments arranged by size:
613 601 232 9
Bsp1286I G_dGCh'C
Cuts at: 0 113 601 610 906 1209 1223 1455
Size: 113 488 9 296 303 14 232
Fragments arranged by size:
488 303 296 232 113 14 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1181 1434 1455
Size: 1181 253 21
Fragments arranged by size:
1181 253 21
BsrBI CCG'CTC
Cuts at: 0 1389 1455
Size: 1389 66
BsrFI r'CCGG_y
Cuts at: 0 1239 1455
Size: 1239 216
BstAPI GCAn_nnn'nTGC
Cuts at: 0 605 1261 1455
Size: 605 656 194
Fragments arranged by size:
656 605 194
BstEII G'GTnAC_C
Cuts at: 0 777 1455
Size: 777 678
BstXI CCAn_nnnn'nTGG
Cuts at: 0 438 805 1455
Size: 438 367 650
Fragments arranged by size:
650 438 367
BstYI r'GATC_y
Cuts at: 0 230 441 1097 1455
Size: 230 211 656 358
Fragments arranged by size:
656 358 230 211
BstZ17I GTA'TAC
Cuts at: 0 641 1455
Size: 641 814
BtgI C'CryG_G
Cuts at: 0 22 86 566 1455
Size: 22 64 480 889
Fragments arranged by size:
889 480 64 22
BtsI GCAGTG_nn'
Cuts at: 0 457 1331 1365 1455
Size: 457 874 34 90
Fragments arranged by size:
874 457 90 34
DraIII CAC_nnn'GTG
Cuts at: 0 605 1455
Size: 605 850
EaeI y'GGCC_r
Cuts at: 0 1415 1455
Size: 1415 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 386 514 650 753 973 1207 1455
Size: 331 55 128 136 103 220 234 248
Fragments arranged by size:
331 248 234 220 136 128 103 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 1339 1455
Size: 118 1221 116
Fragments arranged by size:
1221 118 116
EcoO109I rG'GnC_Cy
Cuts at: 0 495 894 1164 1455
Size: 495 399 270 291
Fragments arranged by size:
495 399 291 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 1455
Size: 900 555
HincII GTy'rAC
Cuts at: 0 156 1455
Size: 156 1299
HpaI GTT'AAC
Cuts at: 0 156 1455
Size: 156 1299
KpnI G_GTAC'C
Cuts at: 0 812 1455
Size: 812 643
MslI CAynn'nnrTG
Cuts at: 0 436 1455
Size: 436 1019
MspA1I CmG'CkG
Cuts at: 0 31 309 490 549 731 960 1142 1148
Size: 31 278 181 59 182 229 182 6
Cuts at: 1148 1356 1424 1455
Size: 208 68 31
Fragments arranged by size:
278 229 208 182 182 181 68 59
31 31 6
NaeI GCC'GGC
Cuts at: 0 1241 1455
Size: 1241 214
NcoI C'CATG_G
Cuts at: 0 86 1455
Size: 86 1369
NgoMIV G'CCGG_C
Cuts at: 0 1239 1455
Size: 1239 216
NspI r_CATG'y
Cuts at: 0 858 1236 1455
Size: 858 378 219
Fragments arranged by size:
858 378 219
PfoI T'CCnGG_A
Cuts at: 0 1100 1455
Size: 1100 355
PpuMI rG'GwC_Cy
Cuts at: 0 495 1455
Size: 495 960
PspXI vC'TCGA_Gb
Cuts at: 0 772 1455
Size: 772 683
PstI C_TGCA'G
Cuts at: 0 596 873 1147 1455
Size: 596 277 274 308
Fragments arranged by size:
596 308 277 274
PvuII CAG'CTG
Cuts at: 0 31 309 490 549 960 1142 1148 1356
Size: 31 278 181 59 411 182 6 208
Cuts at: 1356 1424 1455
Size: 68 31
Fragments arranged by size:
411 278 208 182 181 68 59 31
31 6
SexAI A'CCwGG_T
Cuts at: 0 149 1455
Size: 149 1306
SfcI C'TryA_G
Cuts at: 0 592 869 1143 1455
Size: 592 277 274 312
Fragments arranged by size:
592 312 277 274
SmlI C'TyrA_G
Cuts at: 0 4 126 772 1455
Size: 4 122 646 683
Fragments arranged by size:
683 646 122 4
StuI AGG'CCT
Cuts at: 0 371 1455
Size: 371 1084
StyI C'CwwG_G
Cuts at: 0 57 86 259 353 1373 1455
Size: 57 29 173 94 1020 82
Fragments arranged by size:
1020 173 94 82 57 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 799 1455
Size: 799 656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 287 319 449 481 1455
Size: 287 32 130 32 974
Fragments arranged by size:
974 287 130 32 32
XbaI T'CTAG_A
Cuts at: 0 1272 1455
Size: 1272 183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 1455
Size: 84 1371
XhoI C'TCGA_G
Cuts at: 0 772 1455
Size: 772 683
Enzymes that do cut:
AarI AccI Acc65I AcuI AlfI AlwNI AvaI BamHI
BanI BanII BbvCI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII
BstXI BstYI BstZ17I BtgI BtsI DraIII EaeI Eco57MI
EcoNI EcoO109I FalI HincII HpaI KpnI MslI MspA1I
NaeI NcoI NgoMIV NspI PfoI PpuMI PspXI PstI
PvuII SexAI SfcI SmlI StuI StyI TaqII TstI
XbaI XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BcgI BciVI BclI BfrBI BglI
BmgBI BmtI BsaAI BsaBI BsaHI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HindIII KasI MfeI MluI MmeI MscI NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XmaI XmnI
ZraI