(Circular) (Six-base) MAPSORT of: pFN21AB8841.seq Check: 1511 from: 1 to: 6130 pFN21AB8841 6130 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 2652 2719 2838 4193 5577 5845 1632 Size: 476 544 67 119 1355 1384 268 1917 Fragments arranged by size: 1917 1384 1355 544 476 268 119 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 5029 6006 1203 Size: 3826 977 1327 Fragments arranged by size: 3826 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 3297 1166 Size: 2131 3999 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3301 1184 Size: 2117 4013 AlwNI CAG_nnn'CTG Cuts at: 2080 3400 3873 5445 2080 Size: 1320 473 1572 2765 Fragments arranged by size: 2765 1572 1320 473 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 2055 2467 2600 3439 3665 3817 5926 1493 Size: 562 412 133 839 226 152 2109 1697 Fragments arranged by size: 2109 1697 839 562 412 226 152 133 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2364 3239 3455 1870 Size: 73 421 875 216 4545 Fragments arranged by size: 4545 875 421 216 73 BamHI G'GATC_C Cuts at: 2482 3078 3460 3827 2482 Size: 596 382 367 4785 Fragments arranged by size: 4785 596 382 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2512 2802 3451 4906 618 Size: 325 217 627 725 290 649 1455 1842 Fragments arranged by size: 1842 1455 725 649 627 325 290 217 BanII G_rGCy'C Cuts at: 1391 2187 2463 3449 1391 Size: 796 276 986 4072 Fragments arranged by size: 4072 986 796 276 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2820 2938 4022 5232 2820 Size: 118 1084 1210 3718 Fragments arranged by size: 3718 1210 1084 118 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2228 3404 4741 Size: 107 122 71 631 605 556 1176 1337 Cuts at: 4741 136 Size: 1525 Fragments arranged by size: 1525 1337 1176 631 605 556 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2805 4191 5347 1790 Size: 1015 1386 1156 2573 Fragments arranged by size: 2573 1386 1156 1015 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3067 3861 4819 Size: 416 53 83 399 24 1650 794 958 Cuts at: 4819 442 Size: 1753 Fragments arranged by size: 1753 1650 958 794 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2303 2340 2600 2654 2924 2930 4790 Size: 848 216 37 260 54 270 6 1860 Cuts at: 4790 1239 Size: 2579 Fragments arranged by size: 2579 1860 848 270 260 216 54 37 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2052 2573 3030 4255 5120 5418 2052 Size: 521 457 1225 865 298 2764 Fragments arranged by size: 2764 1225 865 521 457 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 3198 6124 3198 Size: 2926 3204 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2351 3937 4319 4954 Size: 53 83 186 936 818 1586 382 635 Cuts at: 4954 275 Size: 1451 Fragments arranged by size: 1586 1451 936 818 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2922 2952 1178 Size: 30 165 30 1519 30 4356 Fragments arranged by size: 4356 1519 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6130 BseYI C'CCAG_C Cuts at: 2215 2302 2329 3136 5333 5829 2215 Size: 87 27 807 2197 496 2516 Fragments arranged by size: 2516 2197 807 496 87 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 1163 Size: 573 357 5200 Fragments arranged by size: 5200 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2205 2940 4191 4276 5347 2205 Size: 735 1251 85 1071 2988 Fragments arranged by size: 2988 1251 1071 735 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 BsmBI CGTCTCn'nnnn_ Cuts at: 2358 2358 Size: 6130 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2205 2463 2805 2940 3449 4191 Size: 399 397 18 258 342 135 509 742 Cuts at: 4191 4276 5347 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 509 399 397 342 258 135 85 18 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2921 3039 4625 4799 65 Size: 1183 1673 118 1586 174 1396 Fragments arranged by size: 1673 1586 1396 1183 174 118 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstXI CCAn_nnnn'nTGG Cuts at: 2223 2310 2223 Size: 87 6043 BstYI r'GATC_y Cuts at: 1972 2112 2281 2397 2482 3078 3460 3827 4212 Size: 140 169 116 85 596 382 367 385 Cuts at: 4212 4229 5681 5767 5779 6125 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 596 385 382 367 346 169 140 116 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2081 3646 4440 4460 2081 Size: 1565 794 20 3751 Fragments arranged by size: 3751 1565 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2057 2092 529 Size: 35 1493 35 4567 Fragments arranged by size: 4567 1493 35 35 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 3168 3168 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3243 5137 809 Size: 507 1927 1894 1802 Fragments arranged by size: 1927 1894 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095 Size: 54 1425 50 275 18 1747 889 1629 Cuts at: 6095 8 Size: 43 Fragments arranged by size: 1747 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3177 4060 1353 Size: 1824 883 3423 Fragments arranged by size: 3423 1824 883 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2108 2303 2340 2600 2652 2654 Size: 393 455 21 195 37 260 52 2 Cuts at: 2654 2719 2838 2924 2930 4193 4790 5577 5845 Size: 65 119 86 6 1263 597 787 268 Cuts at: 5845 1239 Size: 1524 Fragments arranged by size: 1524 1263 787 597 455 393 268 260 195 119 86 65 52 37 21 6 2 EcoO109I rG'GnC_Cy Cuts at: 2326 2423 2683 3397 2326 Size: 97 260 714 5059 Fragments arranged by size: 5059 714 260 97 EcoRI G'AATT_C Cuts at: 2467 2600 3439 3817 2467 Size: 133 839 378 4780 Fragments arranged by size: 4780 839 378 133 EcoRV GAT'ATC Cuts at: 2893 2893 Size: 6130 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3113 3113 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 5277 1678 Size: 3599 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3107 3139 4771 4803 4845 Size: 32 135 32 1535 32 1632 32 42 Cuts at: 4845 4877 1373 Size: 32 2626 Fragments arranged by size: 2626 1632 1535 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 2753 748 Size: 2005 4125 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 2845 3729 2845 Size: 884 5246 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5244 5428 1305 Size: 3939 184 2007 Fragments arranged by size: 3939 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2933 3297 4088 4447 4606 518 Size: 648 1767 364 791 359 159 2042 Fragments arranged by size: 2042 1767 791 648 364 359 159 MspA1I CmG'CkG Cuts at: 1606 2776 3254 3546 4223 5371 5616 1606 Size: 1170 478 292 677 1148 245 2120 Fragments arranged by size: 2120 1170 1148 677 478 292 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 3488 5033 1132 Size: 97 2259 1545 2229 Fragments arranged by size: 2259 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2292 1348 Size: 944 5186 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PfoI T'CCnGG_A Cuts at: 2319 2940 2319 Size: 621 5509 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 2536 3226 3405 3482 830 Size: 1706 690 179 77 3478 Fragments arranged by size: 3478 1706 690 179 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 2776 3254 2776 Size: 478 5652 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3405 3482 3405 Size: 77 6053 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2532 2703 3222 3401 3478 4613 5294 Size: 219 1487 171 519 179 77 1135 681 Cuts at: 5294 5485 826 Size: 191 1471 Fragments arranged by size: 1487 1471 1135 681 519 219 191 179 171 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 2366 3457 1872 Size: 494 1091 4545 Fragments arranged by size: 4545 1091 494 SmlI C'TyrA_G Cuts at: 820 1017 1943 2067 2552 3045 4234 5135 5397 Size: 197 926 124 485 493 1189 901 262 Cuts at: 5397 820 Size: 1553 Fragments arranged by size: 1553 1189 926 901 493 485 262 197 124 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 2494 4054 5 Size: 47 2442 1560 2081 Fragments arranged by size: 2442 2081 1560 47 StuI AGG'CCT Cuts at: 1517 3422 1517 Size: 1905 4225 StyI C'CwwG_G Cuts at: 513 1065 2004 2142 2178 3004 3569 513 Size: 552 939 138 36 826 565 3074 Fragments arranged by size: 3074 939 826 565 552 138 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2450 4157 4174 4327 4512 4957 325 Size: 1255 870 1707 17 153 185 445 1498 Fragments arranged by size: 1707 1498 1255 870 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4376 6088 96 Size: 274 80 33 51 1443 2399 1712 138 Fragments arranged by size: 2399 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 2364 3455 1870 Size: 494 1091 4545 Fragments arranged by size: 4545 1091 494 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI