(Circular) (Six-base) MAPSORT of: pFN21AB8840.seq Check: 5215 from: 1 to: 6106 pFN21AB8840 6106 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2511 3449 1937 Size: 574 938 4594 Fragments arranged by size: 4594 938 574 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2462 3296 4169 5553 5821 1632 Size: 830 834 873 1384 268 1917 Fragments arranged by size: 1917 1384 873 834 830 268 AflII C'TTAA_G Cuts at: 820 1017 2558 820 Size: 197 1541 4368 Fragments arranged by size: 4368 1541 197 AflIII A'CryG_T Cuts at: 1203 5005 5982 1203 Size: 3802 977 1327 Fragments arranged by size: 3802 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2286 2469 1184 Size: 1102 183 4821 Fragments arranged by size: 4821 1102 183 AlwNI CAG_nnn'CTG Cuts at: 2167 2605 3849 5421 2167 Size: 438 1244 1572 2852 Fragments arranged by size: 2852 1572 1244 438 ApaI G_GGCC'C Cuts at: 2711 3231 2711 Size: 520 5586 ApaLI G'TGCA_C Cuts at: 2042 4163 5319 2042 Size: 2121 1156 2829 Fragments arranged by size: 2829 2121 1156 ApoI r'AATT_y Cuts at: 1493 3415 3641 3793 5902 1493 Size: 1922 226 152 2109 1697 Fragments arranged by size: 2109 1922 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2466 2931 3431 1870 Size: 73 523 465 500 4545 Fragments arranged by size: 4545 523 500 465 73 BamHI G'GATC_C Cuts at: 3342 3436 3803 3342 Size: 94 367 5645 Fragments arranged by size: 5645 367 94 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2582 2763 2834 3427 4882 Size: 325 217 627 795 181 71 593 1455 Cuts at: 4882 618 Size: 1842 Fragments arranged by size: 1842 1455 795 627 593 325 217 181 71 BanII G_rGCy'C Cuts at: 1391 2438 2711 2825 3231 3337 3425 1391 Size: 1047 273 114 406 106 88 4072 Fragments arranged by size: 4072 1047 406 273 114 106 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BbvCI CC'TCA_GC Cuts at: 3183 3183 Size: 6106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4297 4331 1903 Size: 34 2360 34 3678 Fragments arranged by size: 3678 2360 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2138 2900 3068 Size: 107 122 71 631 605 466 762 168 Cuts at: 3068 4717 136 Size: 1649 1525 Fragments arranged by size: 1649 1525 762 631 605 466 168 122 107 71 BglII A'GATC_T Cuts at: 3196 6101 3196 Size: 2905 3201 BlpI GC'TnA_GC Cuts at: 2432 3336 3523 2432 Size: 904 187 5015 Fragments arranged by size: 5015 904 187 Bme1580I G_kGCm'C Cuts at: 1790 2046 2275 2711 2766 2837 2890 3231 4167 Size: 256 229 436 55 71 53 341 936 Cuts at: 4167 5323 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 936 436 341 256 229 71 55 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2793 2799 3837 Size: 416 53 83 399 24 1376 6 1038 Cuts at: 3837 4795 442 Size: 958 1753 Fragments arranged by size: 1753 1376 1038 958 416 399 83 53 24 6 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 3362 4766 1239 Size: 1044 1079 1404 2579 Fragments arranged by size: 2579 1404 1079 1044 Bpu10I CC'TnA_GC Cuts at: 2925 2964 2976 3114 3183 2925 Size: 39 12 138 69 5848 Fragments arranged by size: 5848 138 69 39 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2561 4231 5096 5394 2561 Size: 1670 865 298 3273 Fragments arranged by size: 3273 1670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3913 4295 4930 275 Size: 53 83 186 936 2380 382 635 1451 Fragments arranged by size: 2380 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4539 4937 5211 5358 5769 4539 Size: 398 274 147 411 4876 Fragments arranged by size: 4876 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2281 2311 1178 Size: 30 165 30 878 30 4973 Fragments arranged by size: 4973 878 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2619 3200 1186 Size: 1433 581 4092 Fragments arranged by size: 4092 1433 581 BseYI C'CCAG_C Cuts at: 2142 3078 5309 5805 2142 Size: 936 2231 496 2443 Fragments arranged by size: 2443 2231 936 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2493 1163 Size: 573 757 4776 Fragments arranged by size: 4776 757 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2311 2438 4167 4252 5323 2046 Size: 265 127 1729 85 1071 2829 Fragments arranged by size: 2829 1729 1071 265 127 85 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2275 2311 2438 2711 2766 2825 Size: 399 256 229 36 127 273 55 59 Cuts at: 2825 2837 2890 3231 3337 3425 4167 4252 5323 Size: 12 53 341 106 88 742 85 1071 Cuts at: 5323 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 341 273 256 229 127 106 88 85 59 55 53 36 12 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 3995 5725 3995 Size: 1730 4376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2327 2433 3461 844 Size: 1483 106 1028 3489 Fragments arranged by size: 3489 1483 1028 106 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3383 4601 4775 65 Size: 1183 2135 1218 174 1396 Fragments arranged by size: 2135 1396 1218 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 2268 2883 4166 5178 2268 Size: 615 1283 1012 3196 Fragments arranged by size: 3196 1283 1012 615 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 794 Size: 1373 4733 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3010 2011 Size: 999 5107 BstYI r'GATC_y Cuts at: 1972 2122 3107 3196 3342 3436 3803 4188 4205 Size: 150 985 89 146 94 367 385 17 Cuts at: 4205 5657 5743 5755 6101 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 985 385 367 346 150 146 94 89 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3240 3240 Size: 6106 BtgI C'CryG_G Cuts at: 513 1065 2064 513 Size: 552 999 4555 Fragments arranged by size: 4555 999 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 2093 2209 2348 2484 2603 3622 4416 4436 2093 Size: 116 139 136 119 1019 794 20 3763 Fragments arranged by size: 3763 1019 794 139 136 119 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 2871 3553 Size: 54 1425 50 275 18 549 492 682 Cuts at: 3553 4442 6071 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 682 549 492 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 3164 3206 4036 1353 Size: 729 1082 42 830 3423 Fragments arranged by size: 3423 1082 830 729 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2436 2436 Size: 6106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2462 3296 3362 4169 4766 5553 Size: 393 651 179 834 66 807 597 787 Cuts at: 5553 5821 1239 Size: 268 1524 Fragments arranged by size: 1524 834 807 787 651 597 393 268 179 66 EcoNI CCTnn'n_nnAGG Cuts at: 2087 2087 Size: 6106 EcoO109I rG'GnC_Cy Cuts at: 2080 2164 2545 2707 2708 2732 2793 3228 3254 Size: 84 381 162 1 24 61 435 26 Cuts at: 3254 2080 Size: 4932 Fragments arranged by size: 4932 435 381 162 84 61 26 24 1 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FspI TGC'GCA Cuts at: 2201 4612 2201 Size: 2411 3695 HaeII r_GCGC'y Cuts at: 1678 5253 1678 Size: 3575 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2107 2139 3290 3322 4747 Size: 32 135 32 535 32 1151 32 1425 Cuts at: 4747 4779 4821 4853 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1425 1151 535 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2209 5220 5404 1305 Size: 904 3011 184 2007 Fragments arranged by size: 3011 2007 904 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 2873 10 Size: 54 1425 343 549 492 3243 Fragments arranged by size: 3243 1425 549 492 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4064 4423 4582 518 Size: 648 843 2055 359 159 2042 Fragments arranged by size: 2055 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3331 3340 3522 4199 5347 5592 1606 Size: 1725 9 182 677 1148 245 2120 Fragments arranged by size: 2120 1725 1148 677 245 182 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5554 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2027 2414 3464 5009 1132 Size: 97 798 387 1050 1545 2229 Fragments arranged by size: 2229 1545 1050 798 387 97 PasI CC'CwG_GG Cuts at: 1348 2735 2830 3084 1348 Size: 1387 95 254 4370 Fragments arranged by size: 4370 1387 254 95 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 1108 Size: 1041 5065 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2793 2793 Size: 6106 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PspOMI G'GGCC_C Cuts at: 2707 3227 2707 Size: 520 5586 PstI C_TGCA'G Cuts at: 830 2163 2338 2610 3458 830 Size: 1333 175 272 848 3478 Fragments arranged by size: 3478 1333 848 272 175 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 SacI G_AGCT'C Cuts at: 2438 2438 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SanDI GG'GwC_CC Cuts at: 2793 2793 Size: 6106 SbfI CC_TGCA'GG Cuts at: 2163 2338 3458 2163 Size: 175 1120 4811 Fragments arranged by size: 4811 1120 175 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SfcI C'TryA_G Cuts at: 826 1045 2159 2221 2334 2606 3454 4589 5270 Size: 219 1114 62 113 272 848 1135 681 Cuts at: 5270 5461 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1114 848 681 272 219 191 113 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 2468 2933 3433 1872 Size: 596 465 500 4545 Fragments arranged by size: 4545 596 500 465 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 2558 4210 5111 5373 820 Size: 197 926 597 18 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 597 262 197 18 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 2414 3464 1132 Size: 1282 1050 3774 Fragments arranged by size: 3774 1282 1050 SrfI GCCC'GGGC Cuts at: 2933 2933 Size: 6106 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 2853 2895 2916 2937 2943 2997 3024 Size: 552 1788 42 21 21 6 54 27 Cuts at: 3024 3030 3042 3057 3063 3545 513 Size: 6 12 15 6 482 3074 Fragments arranged by size: 3074 1788 552 482 54 42 27 21 21 15 12 6 6 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2507 2570 4133 4150 4303 4488 4933 Size: 1255 927 63 1563 17 153 185 445 Cuts at: 4933 325 Size: 1498 Fragments arranged by size: 1563 1498 1255 927 445 185 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4352 6064 96 Size: 274 80 33 51 1443 2375 1712 138 Fragments arranged by size: 2375 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2224 2256 2281 2313 2338 2370 6066 6098 2224 Size: 32 25 32 25 32 3696 32 2232 Fragments arranged by size: 3696 2232 32 32 32 32 25 25 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2575 2950 3064 2011 Size: 564 375 114 5053 Fragments arranged by size: 5053 564 375 114 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 2466 2931 3431 1870 Size: 596 465 500 4545 Fragments arranged by size: 4545 596 500 465 XmnI GAAnn'nnTTC Cuts at: 4235 4235 Size: 6106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SwaI