(Circular) (Six-base) MAPSORT of: pFN21AB8838.seq Check: 1678 from: 1 to: 6094 pFN21AB8838 6094 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2323 2323 Size: 6094 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3135 3904 278 Size: 53 83 186 936 1599 769 2468 Fragments arranged by size: 2468 1599 936 769 186 83 53 AccI GT'mk_AC Cuts at: 1937 3437 1937 Size: 1500 4594 Acc65I G'GTAC_C Cuts at: 3415 3415 Size: 6094 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 4157 5541 5809 1632 Size: 557 1968 1384 268 1917 Fragments arranged by size: 1968 1917 1384 557 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5897 AflIII A'CryG_T Cuts at: 1203 4993 5970 1203 Size: 3790 977 1327 Fragments arranged by size: 3790 1327 977 AgeI A'CCGG_T Cuts at: 3104 4925 3104 Size: 1821 4273 AhdI GACnn_n'nnGTC Cuts at: 2380 4823 2380 Size: 2443 3651 AleI CACnn'nnGTG Cuts at: 1166 3059 1166 Size: 1893 4201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3077 1184 Size: 1893 4201 AlwNI CAG_nnn'CTG Cuts at: 2251 2320 2833 3231 3837 5409 2251 Size: 69 513 398 606 1572 2936 Fragments arranged by size: 2936 1572 606 513 398 69 ApaI G_GGCC'C Cuts at: 2198 2806 3142 2198 Size: 608 336 5150 Fragments arranged by size: 5150 608 336 ApaLI G'TGCA_C Cuts at: 4151 5307 4151 Size: 1156 4938 ApoI r'AATT_y Cuts at: 1493 3146 3403 3629 3781 5890 1493 Size: 1653 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 1653 257 226 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 2255 3042 3419 1870 Size: 73 312 787 377 4545 Fragments arranged by size: 4545 787 377 312 73 BamHI G'GATC_C Cuts at: 2551 3424 3791 2551 Size: 873 367 4854 Fragments arranged by size: 4854 873 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2345 3101 3415 4870 Size: 325 217 627 273 285 756 314 1455 Cuts at: 4870 618 Size: 1842 Fragments arranged by size: 1842 1455 756 627 325 314 285 273 217 BanII G_rGCy'C Cuts at: 1391 2072 2198 2806 2978 3116 3142 3413 1391 Size: 681 126 608 172 138 26 271 4072 Fragments arranged by size: 4072 681 608 271 172 138 126 26 BbeI G_GCGC'C Cuts at: 2064 2064 Size: 6094 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4285 4319 1903 Size: 34 2348 34 3678 Fragments arranged by size: 3678 2348 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3986 5196 3986 Size: 1210 4884 BclI T'GATC_A Cuts at: 1548 1548 Size: 6094 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2432 4705 136 Size: 107 122 71 631 605 760 2273 1525 Fragments arranged by size: 2273 1525 760 631 605 122 107 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 3071 3511 3071 Size: 440 5654 Bme1580I G_kGCm'C Cuts at: 1790 2198 2291 2806 3104 3142 4155 5311 1790 Size: 408 93 515 298 38 1013 1156 2573 Fragments arranged by size: 2573 1156 1013 515 408 298 93 38 BmgBI CAC'GTC Cuts at: 1318 2377 2989 1318 Size: 1059 612 4423 Fragments arranged by size: 4423 1059 612 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2778 2843 3825 Size: 416 53 83 399 24 1361 65 982 Cuts at: 3825 4783 442 Size: 958 1753 Fragments arranged by size: 1753 1361 982 958 416 399 83 65 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3251 3251 Size: 6094 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2300 2311 3173 3197 3409 4754 1239 Size: 953 108 11 862 24 212 1345 2579 Fragments arranged by size: 2579 1345 953 862 212 108 24 11 Bpu10I CC'TnA_GC Cuts at: 3209 3209 Size: 6094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 4219 5084 5382 2105 Size: 2114 865 298 2817 Fragments arranged by size: 2817 2114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2723 4757 882 Size: 617 1224 2034 2219 Fragments arranged by size: 2219 2034 1224 617 BsaAI yAC'GTr Cuts at: 493 1204 3885 493 Size: 711 2681 2702 Fragments arranged by size: 2702 2681 711 BsaBI GATnn'nnATC Cuts at: 6088 6088 Size: 6094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 3132 3901 4283 Size: 53 83 186 936 528 1071 769 382 Cuts at: 4283 4918 275 Size: 635 1451 Fragments arranged by size: 1451 1071 936 769 635 528 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3104 4527 4925 5199 5346 5757 3104 Size: 1423 398 274 147 411 3441 Fragments arranged by size: 3441 1423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5869 Fragments arranged by size: 5869 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6094 BseYI C'CCAG_C Cuts at: 2010 2284 3341 5297 5793 2010 Size: 274 1057 1956 496 2311 Fragments arranged by size: 2311 1956 1057 496 274 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5521 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2052 2138 2572 3068 3544 4305 Size: 275 186 51 86 434 496 476 761 Cuts at: 4305 5333 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 496 476 434 275 186 86 51 BsiHKAI G_wGCw'C Cuts at: 2072 4155 4240 5311 2072 Size: 2083 85 1071 2855 Fragments arranged by size: 2855 2083 1071 85 BsmI GAATG_Cn' Cuts at: 2400 3605 3698 2400 Size: 1205 93 4796 Fragments arranged by size: 4796 1205 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2995 2995 Size: 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2198 2291 2806 2978 3104 3116 Size: 399 282 126 93 515 172 126 12 Cuts at: 3116 3142 3413 4155 4240 5311 1391 Size: 26 271 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 399 282 271 172 126 126 93 85 26 12 BspEI T'CCGG_A Cuts at: 5757 5757 Size: 6094 BspHI T'CATG_A Cuts at: 3983 5713 3983 Size: 1730 4364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2323 3202 3449 844 Size: 1479 879 247 3489 Fragments arranged by size: 3489 1479 879 247 BsrBI CCG'CTC Cuts at: 1394 1823 2634 2840 3981 1394 Size: 429 811 206 1141 3507 Fragments arranged by size: 3507 1141 811 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 4589 4763 65 Size: 1183 3341 174 1396 Fragments arranged by size: 3341 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2137 2501 3104 4738 4925 1700 Size: 110 327 364 603 1634 187 2869 Fragments arranged by size: 2869 1634 603 364 327 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6094 BssHII G'CGCG_C Cuts at: 1925 2396 1925 Size: 471 5623 BssSI C'ACGA_G Cuts at: 4154 5166 4154 Size: 1012 5082 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6094 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6094 BstXI CCAn_nnnn'nTGG Cuts at: 2335 2524 2335 Size: 189 5905 BstYI r'GATC_y Cuts at: 1972 2551 2592 3001 3424 3791 4176 4193 5645 Size: 579 41 409 423 367 385 17 1452 Cuts at: 5645 5731 5743 6089 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 579 423 409 385 367 346 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 2575 2970 3367 513 Size: 552 939 42 529 395 397 3240 Fragments arranged by size: 3240 939 552 529 397 395 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2377 487 Size: 683 56 1151 4204 Fragments arranged by size: 4204 1151 683 56 BtsI GCAGTG_nn' Cuts at: 3304 3610 4404 4424 3304 Size: 306 794 20 4974 Fragments arranged by size: 4974 794 306 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6059 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DraIII CAC_nnn'GTG Cuts at: 2352 2352 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2135 2496 2526 Size: 54 1425 50 275 18 305 361 30 Cuts at: 2526 2578 3541 4430 6059 8 Size: 52 963 889 1629 43 Fragments arranged by size: 1629 1425 963 889 361 305 275 54 52 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2135 3541 1537 Size: 275 323 1406 4090 Fragments arranged by size: 4090 1406 323 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3128 3190 4024 1353 Size: 1775 62 834 3423 Fragments arranged by size: 3423 1775 834 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2680 3157 4681 5055 5201 387 Size: 1064 138 1091 477 1524 374 146 1280 Fragments arranged by size: 1524 1280 1091 1064 477 374 146 138 EcoICRI GAG'CTC Cuts at: 2070 2070 Size: 6094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2192 2300 2311 3173 3197 3409 Size: 393 557 3 108 11 862 24 212 Cuts at: 3409 4157 4754 5541 5809 1239 Size: 748 597 787 268 1524 Fragments arranged by size: 1524 862 787 748 597 557 393 268 212 108 24 11 3 EcoO109I rG'GnC_Cy Cuts at: 2194 2195 2803 3191 3347 2194 Size: 1 608 388 156 4941 Fragments arranged by size: 4941 608 388 156 1 EcoRI G'AATT_C Cuts at: 3403 3781 3403 Size: 378 5716 FspI TGC'GCA Cuts at: 4600 4600 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 2045 2064 2275 2660 5241 1678 Size: 367 19 211 385 2581 2531 Fragments arranged by size: 2581 2531 385 367 211 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 2942 2974 4735 Size: 32 135 32 794 32 544 32 1761 Cuts at: 4735 4767 4809 4841 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1761 794 544 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2296 3438 3684 669 Size: 988 281 358 1142 246 3079 Fragments arranged by size: 3079 1142 988 358 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6094 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KasI G'GCGC_C Cuts at: 2060 2060 Size: 6094 KpnI G_GTAC'C Cuts at: 3419 3419 Size: 6094 MfeI C'AATT_G Cuts at: 3693 3693 Size: 6094 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5208 5392 1305 Size: 3903 184 2007 Fragments arranged by size: 3903 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 2528 2580 10 Size: 54 1425 343 666 30 52 3524 Fragments arranged by size: 3524 1425 666 343 54 52 30 MslI CAynn'nnrTG Cuts at: 518 1166 3059 3095 3278 4052 4411 4570 518 Size: 648 1893 36 183 774 359 159 2042 Fragments arranged by size: 2042 1893 774 648 359 183 159 36 MspA1I CmG'CkG Cuts at: 1606 2048 2096 2113 2539 3510 4187 5335 5580 Size: 442 48 17 426 971 677 1148 245 Cuts at: 5580 1606 Size: 2120 Fragments arranged by size: 2120 1148 971 677 442 426 245 48 17 NaeI GCC'GGC Cuts at: 2139 2139 Size: 6094 NarI GG'CG_CC Cuts at: 2061 2061 Size: 6094 NcoI C'CATG_G Cuts at: 513 1065 2004 2575 3367 513 Size: 552 939 571 792 3240 Fragments arranged by size: 3240 939 792 571 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6094 NgoMIV G'CCGG_C Cuts at: 2137 2137 Size: 6094 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 3452 4997 1132 Size: 97 2223 1545 2229 Fragments arranged by size: 2229 2223 1545 97 PasI CC'CwG_GG Cuts at: 1348 2766 1348 Size: 1418 4676 PciI A'CATG_T Cuts at: 4993 4993 Size: 6094 PflMI CCAn_nnn'nTGG Cuts at: 1108 2833 1108 Size: 1725 4369 PfoI T'CCnGG_A Cuts at: 2922 2997 3150 2922 Size: 75 153 5866 Fragments arranged by size: 5866 153 75 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3191 3191 Size: 6094 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6094 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PspOMI G'GGCC_C Cuts at: 2194 2802 3138 2194 Size: 608 336 5150 Fragments arranged by size: 5150 608 336 PstI C_TGCA'G Cuts at: 830 3361 3446 830 Size: 2531 85 3478 Fragments arranged by size: 3478 2531 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 PvuII CAG'CTG Cuts at: 2539 2539 Size: 6094 SacI G_AGCT'C Cuts at: 2072 2072 Size: 6094 SacII CC_GC'GG Cuts at: 2049 2049 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SapI GCTCTTCn'nnn_ Cuts at: 3128 3128 Size: 6094 SbfI CC_TGCA'GG Cuts at: 3361 3446 3361 Size: 85 6009 ScaI AGT'ACT Cuts at: 4342 6054 4342 Size: 1712 4382 SfcI C'TryA_G Cuts at: 826 1045 3357 3442 4577 5258 5449 826 Size: 219 2312 85 1135 681 191 1471 Fragments arranged by size: 2312 1471 1135 681 219 191 85 SfiI GGCCn_nnn'nGGCC Cuts at: 2432 2432 Size: 6094 SfoI GGC'GCC Cuts at: 2062 2062 Size: 6094 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 2501 1700 Size: 801 5293 SmaI CCC'GGG Cuts at: 1872 3421 1872 Size: 1549 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 3042 4198 5099 5361 820 Size: 197 926 177 922 1156 901 262 1553 Fragments arranged by size: 1553 1156 926 922 901 262 197 177 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 3452 1132 Size: 2320 3774 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 3363 3384 1517 Size: 1846 21 4227 Fragments arranged by size: 4227 1846 21 StyI C'CwwG_G Cuts at: 513 1065 2004 2575 2847 3367 3533 513 Size: 552 939 571 272 520 166 3074 Fragments arranged by size: 3074 939 571 552 520 272 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3244 3317 4121 4138 4291 4476 4921 Size: 1255 1664 73 804 17 153 185 445 Cuts at: 4921 325 Size: 1498 Fragments arranged by size: 1664 1498 1255 804 445 185 153 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4340 6052 96 Size: 274 80 33 51 1443 2363 1712 138 Fragments arranged by size: 2363 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 2918 2950 6054 6086 2543 Size: 32 343 32 3104 32 2551 Fragments arranged by size: 3104 2551 343 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6094 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2216 2491 2216 Size: 275 5819 XhoI C'TCGA_G Cuts at: 1943 3042 1943 Size: 1099 4995 XmaI C'CCGG_G Cuts at: 1870 3419 1870 Size: 1549 4545 XmnI GAAnn'nnTTC Cuts at: 3282 4223 3282 Size: 941 5153 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3133 3902 276 Size: 53 83 186 936 1599 769 2468 Fragments arranged by size: 2468 1599 936 769 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SrfI SwaI