(Circular) (Six-base) MAPSORT of: pFN21AB8837.seq Check: 2262 from: 1 to: 6094 pFN21AB8837 6094 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2947 2963 2947 Size: 16 6078 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3904 278 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2391 3147 3437 1937 Size: 454 756 290 4594 Fragments arranged by size: 4594 756 454 290 Acc65I G'GTAC_C Cuts at: 3415 3415 Size: 6094 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2380 2542 4157 5541 5809 1632 Size: 748 162 1615 1384 268 1917 Fragments arranged by size: 1917 1615 1384 748 268 162 AflII C'TTAA_G Cuts at: 820 1017 3347 820 Size: 197 2330 3567 Fragments arranged by size: 3567 2330 197 AflIII A'CryG_T Cuts at: 1203 4993 5970 1203 Size: 3790 977 1327 Fragments arranged by size: 3790 1327 977 AgeI A'CCGG_T Cuts at: 4925 4925 Size: 6094 AhdI GACnn_n'nnGTC Cuts at: 4823 4823 Size: 6094 AleI CACnn'nnGTG Cuts at: 1166 2510 2687 1166 Size: 1344 177 4573 Fragments arranged by size: 4573 1344 177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6094 AlwNI CAG_nnn'CTG Cuts at: 2140 2842 3837 5409 2140 Size: 702 995 1572 2825 Fragments arranged by size: 2825 1572 995 702 ApaLI G'TGCA_C Cuts at: 4151 5307 4151 Size: 1156 4938 ApoI r'AATT_y Cuts at: 1493 3403 3629 3781 5890 1493 Size: 1910 226 152 2109 1697 Fragments arranged by size: 2109 1910 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 3419 1870 Size: 73 1476 4545 Fragments arranged by size: 4545 1476 73 BamHI G'GATC_C Cuts at: 3424 3791 3424 Size: 367 5727 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2724 3062 3415 4870 Size: 325 217 627 242 695 338 353 1455 Cuts at: 4870 618 Size: 1842 Fragments arranged by size: 1842 1455 695 627 353 338 325 242 217 BanII G_rGCy'C Cuts at: 1391 3287 3413 1391 Size: 1896 126 4072 Fragments arranged by size: 4072 1896 126 BbeI G_GCGC'C Cuts at: 2728 3066 2728 Size: 338 5756 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5123 BbvCI CC'TCA_GC Cuts at: 3183 3183 Size: 6094 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3316 3350 4285 4319 1903 Size: 34 1379 34 935 34 3678 Fragments arranged by size: 3678 1379 935 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3986 5196 3986 Size: 1210 4884 BclI T'GATC_A Cuts at: 1548 1548 Size: 6094 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2624 2733 2948 Size: 107 122 71 631 605 952 109 215 Cuts at: 2948 4705 136 Size: 1757 1525 Fragments arranged by size: 1757 1525 952 631 605 215 122 109 107 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 3511 3511 Size: 6094 Bme1580I G_kGCm'C Cuts at: 1790 4155 5311 1790 Size: 2365 1156 2573 Fragments arranged by size: 2573 2365 1156 BmgBI CAC'GTC Cuts at: 1318 2323 1318 Size: 1005 5089 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3043 3825 4783 Size: 416 53 83 399 24 1626 782 958 Cuts at: 4783 442 Size: 1753 Fragments arranged by size: 1753 1626 958 782 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2117 2189 2255 2340 2783 3143 3236 Size: 857 21 72 66 85 443 360 93 Cuts at: 3236 4754 1239 Size: 1518 2579 Fragments arranged by size: 2579 1518 857 443 360 93 85 72 66 21 Bpu10I CC'TnA_GC Cuts at: 3183 3183 Size: 6094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2792 4219 5084 5382 2792 Size: 1427 865 298 3504 Fragments arranged by size: 3504 1427 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2270 2428 2444 2583 3022 4757 882 Size: 617 771 158 16 139 439 1735 2219 Fragments arranged by size: 2219 1735 771 617 439 158 139 16 BsaAI yAC'GTr Cuts at: 493 1204 3885 493 Size: 711 2681 2702 Fragments arranged by size: 2702 2681 711 BsaBI GATnn'nnATC Cuts at: 6088 6088 Size: 6094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2178 2268 2725 2829 Size: 53 83 186 936 645 90 457 104 Cuts at: 2829 3063 3189 3901 4283 4918 275 Size: 234 126 712 382 635 1451 Fragments arranged by size: 1451 936 712 645 635 457 382 234 186 126 104 90 83 53 BsaWI w'CCGG_w Cuts at: 4527 4925 5199 5346 5757 4527 Size: 398 274 147 411 4864 Fragments arranged by size: 4864 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2699 2729 1178 Size: 30 165 30 1296 30 4543 Fragments arranged by size: 4543 1296 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 1186 Size: 868 5226 BseYI C'CCAG_C Cuts at: 2541 3192 5297 5793 2541 Size: 651 2105 496 2842 Fragments arranged by size: 2842 2105 651 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5521 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2549 2865 3544 4305 5333 1540 Size: 275 186 548 316 679 761 1028 2301 Fragments arranged by size: 2301 1028 761 679 548 316 275 186 BsiHKAI G_wGCw'C Cuts at: 2706 4155 4240 5311 2706 Size: 1449 85 1071 3489 Fragments arranged by size: 3489 1449 1071 85 BsmI GAATG_Cn' Cuts at: 3605 3698 3605 Size: 93 6001 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2706 3287 3413 4155 4240 5311 1391 Size: 399 916 581 126 742 85 1071 2174 Fragments arranged by size: 2174 1071 916 742 581 399 126 85 BspEI T'CCGG_A Cuts at: 5757 5757 Size: 6094 BspHI T'CATG_A Cuts at: 3983 5713 3983 Size: 1730 4364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2377 2718 2947 2963 2988 3332 3449 Size: 1502 31 341 229 16 25 344 117 Cuts at: 3449 844 Size: 3489 Fragments arranged by size: 3489 1502 344 341 229 117 31 25 16 BsrBI CCG'CTC Cuts at: 1394 1823 2605 2997 3981 1394 Size: 429 782 392 984 3507 Fragments arranged by size: 3507 984 782 429 392 BsrDI GCAATG_nn' Cuts at: 65 1248 4589 4763 65 Size: 1183 3341 174 1396 Fragments arranged by size: 3341 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2348 2618 2662 2721 2845 4738 4925 Size: 110 538 270 44 59 124 1893 187 Cuts at: 4925 1700 Size: 2869 Fragments arranged by size: 2869 1893 538 270 187 124 110 59 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 4154 5166 4154 Size: 1012 5082 BstAPI GCAn_nnn'nTGC Cuts at: 794 2710 794 Size: 1916 4178 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 2105 2975 1184 Size: 921 870 4303 Fragments arranged by size: 4303 921 870 BstXI CCAn_nnnn'nTGG Cuts at: 2059 3073 2059 Size: 1014 5080 BstYI r'GATC_y Cuts at: 1972 2469 2583 3424 3791 4176 4193 5645 5731 Size: 497 114 841 367 385 17 1452 86 Cuts at: 5731 5743 6089 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 841 497 385 367 346 114 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2151 513 Size: 552 939 21 126 4456 Fragments arranged by size: 4456 939 552 126 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5355 Fragments arranged by size: 5355 683 56 BtsI GCAGTG_nn' Cuts at: 2894 3610 4404 4424 2894 Size: 716 794 20 4564 Fragments arranged by size: 4564 794 716 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6059 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DraIII CAC_nnn'GTG Cuts at: 2900 2900 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2460 2529 Size: 54 1425 50 275 18 231 399 69 Cuts at: 2529 2546 2862 2940 2949 3006 3255 3541 4430 Size: 17 316 78 9 57 249 286 889 Cuts at: 4430 6059 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 399 316 286 275 249 231 78 69 57 54 50 43 18 17 9 EagI C'GGCC_G Cuts at: 1537 1812 2546 2862 3541 1537 Size: 275 734 316 679 4090 Fragments arranged by size: 4090 734 679 316 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3381 4024 1353 Size: 2028 643 3423 Fragments arranged by size: 3423 2028 643 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2153 4681 5055 5201 387 Size: 1064 138 564 2528 374 146 1280 Fragments arranged by size: 2528 1280 1064 564 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2117 2189 2255 2340 2380 2542 Size: 393 464 21 72 66 85 40 162 Cuts at: 2542 2783 3143 3236 4157 4754 5541 5809 1239 Size: 241 360 93 921 597 787 268 1524 Fragments arranged by size: 1524 921 787 597 464 393 360 268 241 162 93 85 72 66 40 21 EcoNI CCTnn'n_nnAGG Cuts at: 2114 2384 2477 3088 2114 Size: 270 93 611 5120 Fragments arranged by size: 5120 611 270 93 EcoO109I rG'GnC_Cy Cuts at: 2080 2245 2335 2521 2839 3211 2080 Size: 165 90 186 318 372 4963 Fragments arranged by size: 4963 372 318 186 165 90 EcoRI G'AATT_C Cuts at: 3403 3781 3403 Size: 378 5716 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2512 2512 Size: 6094 FspI TGC'GCA Cuts at: 2087 2504 4600 2087 Size: 417 2096 3581 Fragments arranged by size: 3581 2096 417 FspAI rTGC'GCAy Cuts at: 2504 2504 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 2728 3066 3144 5241 1678 Size: 1050 338 78 2097 2531 Fragments arranged by size: 2531 2097 1050 338 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2699 2731 2918 2950 2969 Size: 32 135 32 1127 32 187 32 19 Cuts at: 2969 3001 4735 4767 4809 4841 1373 Size: 32 1734 32 42 32 2626 Fragments arranged by size: 2626 1734 1127 187 135 42 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 3438 3684 669 Size: 988 281 1500 246 3079 Fragments arranged by size: 3079 1500 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6094 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KasI G'GCGC_C Cuts at: 2724 3062 2724 Size: 338 5756 KpnI G_GTAC'C Cuts at: 3419 3419 Size: 6094 MfeI C'AATT_G Cuts at: 3693 3693 Size: 6094 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2676 3081 5208 5392 1305 Size: 1371 405 2127 184 2007 Fragments arranged by size: 2127 2007 1371 405 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2063 2942 3008 3257 10 Size: 54 1425 343 231 879 66 249 2847 Fragments arranged by size: 2847 1425 879 343 249 231 66 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2510 2687 3071 3101 4052 4411 Size: 648 891 453 177 384 30 951 359 Cuts at: 4411 4570 518 Size: 159 2042 Fragments arranged by size: 2042 951 891 648 453 384 359 177 159 30 MspA1I CmG'CkG Cuts at: 1606 2436 2465 2545 2570 3366 3510 4187 5335 Size: 830 29 80 25 796 144 677 1148 Cuts at: 5335 5580 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 830 796 677 245 144 80 29 25 NaeI GCC'GGC Cuts at: 2350 2620 2664 2350 Size: 270 44 5780 Fragments arranged by size: 5780 270 44 NarI GG'CG_CC Cuts at: 2725 3063 2725 Size: 338 5756 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4603 Fragments arranged by size: 4603 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6094 NgoMIV G'CCGG_C Cuts at: 2348 2618 2662 2348 Size: 270 44 5780 Fragments arranged by size: 5780 270 44 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 2862 3541 2862 Size: 679 5415 NspI r_CATG'y Cuts at: 1132 1229 3452 4997 1132 Size: 97 2223 1545 2229 Fragments arranged by size: 2229 2223 1545 97 PasI CC'CwG_GG Cuts at: 1348 2852 1348 Size: 1504 4590 PciI A'CATG_T Cuts at: 4993 4993 Size: 6094 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6094 PfoI T'CCnGG_A Cuts at: 2133 2133 Size: 6094 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2335 2521 2245 Size: 90 186 5818 Fragments arranged by size: 5818 186 90 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6094 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PstI C_TGCA'G Cuts at: 830 2580 3329 3446 830 Size: 1750 749 117 3478 Fragments arranged by size: 3478 1750 749 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 PvuII CAG'CTG Cuts at: 3366 3366 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SbfI CC_TGCA'GG Cuts at: 2580 3329 3446 2580 Size: 749 117 5228 Fragments arranged by size: 5228 749 117 ScaI AGT'ACT Cuts at: 4342 6054 4342 Size: 1712 4382 SfcI C'TryA_G Cuts at: 826 1045 2576 3325 3442 4577 5258 5449 826 Size: 219 1531 749 117 1135 681 191 1471 Fragments arranged by size: 1531 1471 1135 749 681 219 191 117 SfiI GGCCn_nnn'nGGCC Cuts at: 2948 2948 Size: 6094 SfoI GGC'GCC Cuts at: 2726 3064 2726 Size: 338 5756 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 2721 1700 Size: 1021 5073 SmaI CCC'GGG Cuts at: 1872 3421 1872 Size: 1549 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2807 3347 4198 5099 5361 820 Size: 197 926 864 540 851 901 262 1553 Fragments arranged by size: 1553 926 901 864 851 540 262 197 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 3452 1132 Size: 2320 3774 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 2768 1517 Size: 1251 4843 StyI C'CwwG_G Cuts at: 513 1065 2004 2064 2508 3533 513 Size: 552 939 60 444 1025 3074 Fragments arranged by size: 3074 1025 939 552 444 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2322 2555 2977 4121 4138 4291 4476 Size: 1255 742 233 422 1144 17 153 185 Cuts at: 4476 4921 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1144 742 445 422 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3351 4340 6052 Size: 274 80 33 51 1443 1374 989 1712 Cuts at: 6052 96 Size: 138 Fragments arranged by size: 1712 1443 1374 989 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6054 6086 6054 Size: 32 6062 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6094 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2071 2897 2915 2071 Size: 826 18 5250 Fragments arranged by size: 5250 826 18 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6094 XmaI C'CCGG_G Cuts at: 1870 3419 1870 Size: 1549 4545 XmnI GAAnn'nnTTC Cuts at: 4223 4223 Size: 6094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3902 276 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI