(Circular) (Six-base) MAPSORT of: pFN21AB8833.seq Check: 2617 from: 1 to: 6064 pFN21AB8833 6064 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2076 2135 3407 1937 Size: 139 59 1272 4594 Fragments arranged by size: 4594 1272 139 59 Acc65I G'GTAC_C Cuts at: 2393 3385 2393 Size: 992 5072 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 2369 4127 5511 5779 1632 Size: 566 171 1758 1384 268 1917 Fragments arranged by size: 1917 1758 1384 566 268 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 4963 5940 1203 Size: 3760 977 1327 Fragments arranged by size: 3760 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 2131 4793 2131 Size: 2662 3402 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2644 2676 2644 Size: 32 6032 AlwNI CAG_nnn'CTG Cuts at: 2201 3807 5379 2201 Size: 1606 1572 2886 Fragments arranged by size: 2886 1606 1572 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 3236 3373 3599 3751 5860 1493 Size: 1743 137 226 152 2109 1697 Fragments arranged by size: 2109 1743 1697 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2255 3389 1870 Size: 73 73 239 1134 4545 Fragments arranged by size: 4545 1134 239 73 73 BamHI G'GATC_C Cuts at: 2294 3394 3761 2294 Size: 1100 367 4597 Fragments arranged by size: 4597 1100 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2393 3385 4840 618 Size: 325 217 627 273 333 992 1455 1842 Fragments arranged by size: 1842 1455 992 627 333 325 273 217 BanII G_rGCy'C Cuts at: 1391 2545 3219 3383 1391 Size: 1154 674 164 4072 Fragments arranged by size: 4072 1154 674 164 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 928 Size: 971 231 4862 Fragments arranged by size: 4862 971 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4255 4289 1903 Size: 34 2318 34 3678 Fragments arranged by size: 3678 2318 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3956 5166 3956 Size: 1210 4854 BclI T'GATC_A Cuts at: 1548 1548 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2442 4675 136 Size: 107 122 71 631 605 770 2233 1525 Fragments arranged by size: 2233 1525 770 631 605 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 4125 5281 1790 Size: 2335 1156 2573 Fragments arranged by size: 2573 2335 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3795 4753 442 Size: 416 53 83 399 24 2378 958 1753 Fragments arranged by size: 2378 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2529 2666 3297 4724 1239 Size: 1290 137 631 1427 2579 Fragments arranged by size: 2579 1427 1290 631 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2845 4189 5054 5352 2845 Size: 1344 865 298 3557 Fragments arranged by size: 3557 1344 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4727 882 Size: 617 3228 2219 Fragments arranged by size: 3228 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2735 3855 493 Size: 711 1531 1120 2702 Fragments arranged by size: 2702 1531 1120 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3871 4253 4888 275 Size: 53 83 186 936 2338 382 635 1451 Fragments arranged by size: 2338 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2565 4497 4895 5169 5316 5727 2565 Size: 1932 398 274 147 411 2902 Fragments arranged by size: 2902 1932 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2527 2557 3270 3300 1178 Size: 30 165 30 1124 30 713 30 3942 Fragments arranged by size: 3942 1124 713 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 1186 Size: 958 5106 BseYI C'CCAG_C Cuts at: 2189 2924 5267 5763 2189 Size: 735 2343 496 2490 Fragments arranged by size: 2490 2343 735 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2936 1163 Size: 573 1200 4291 Fragments arranged by size: 4291 1200 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2537 2617 4125 4210 5281 2537 Size: 80 1508 85 1071 3320 Fragments arranged by size: 3320 1508 1071 85 80 BsmI GAATG_Cn' Cuts at: 2731 3575 3668 2731 Size: 844 93 5127 Fragments arranged by size: 5127 844 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2091 2091 Size: 6064 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2537 2545 2617 3219 3383 4125 4210 Size: 399 747 8 72 602 164 742 85 Cuts at: 4210 5281 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 747 742 602 399 164 85 72 8 BspEI T'CCGG_A Cuts at: 2565 5727 2565 Size: 3162 2902 BspHI T'CATG_A Cuts at: 3953 5683 3953 Size: 1730 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3419 844 Size: 2575 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3951 1394 Size: 429 2128 3507 Fragments arranged by size: 3507 2128 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6064 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstYI r'GATC_y Cuts at: 1972 2294 2477 3394 3761 4146 4163 5615 5701 Size: 322 183 917 367 385 17 1452 86 Cuts at: 5701 5713 6059 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 917 385 367 346 322 183 86 17 12 BstZ17I GTA'TAC Cuts at: 2077 2077 Size: 6064 Bsu36I CC'TnA_GG Cuts at: 2238 2238 Size: 6064 BtgI C'CryG_G Cuts at: 513 1065 2004 2889 513 Size: 552 939 885 3688 Fragments arranged by size: 3688 939 885 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5325 Fragments arranged by size: 5325 683 56 BtsI GCAGTG_nn' Cuts at: 3580 4374 4394 3580 Size: 794 20 5250 Fragments arranged by size: 5250 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2891 5071 809 Size: 507 1575 2180 1802 Fragments arranged by size: 2180 1802 1575 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 3511 4400 Size: 54 1425 50 275 18 204 1477 889 Cuts at: 4400 6029 8 Size: 1629 43 Fragments arranged by size: 1629 1477 1425 889 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2089 3994 1353 Size: 736 1905 3423 Fragments arranged by size: 3423 1905 736 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4651 5025 5171 387 Size: 1064 138 3062 374 146 1280 Fragments arranged by size: 3062 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2369 2529 2666 3297 4127 4724 Size: 393 566 171 160 137 631 830 597 Cuts at: 4724 5511 5779 1239 Size: 787 268 1524 Fragments arranged by size: 1524 830 787 631 597 566 393 268 171 160 137 EcoNI CCTnn'n_nnAGG Cuts at: 2627 2627 Size: 6064 EcoO109I rG'GnC_Cy Cuts at: 2332 2412 2632 2332 Size: 80 220 5764 Fragments arranged by size: 5764 220 80 EcoRI G'AATT_C Cuts at: 3236 3373 3751 3236 Size: 137 378 5549 Fragments arranged by size: 5549 378 137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2599 2599 Size: 6064 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 5211 1678 Size: 3533 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 3081 3113 4705 Size: 32 135 32 545 32 932 32 1592 Cuts at: 4705 4737 4779 4811 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1592 932 545 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3408 3654 669 Size: 988 281 1002 468 246 3079 Fragments arranged by size: 3079 1002 988 468 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 2940 3654 2940 Size: 714 5350 KpnI G_GTAC'C Cuts at: 2397 3389 2397 Size: 992 5072 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4242 Fragments arranged by size: 4242 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2951 4022 4381 4540 518 Size: 648 1785 1071 359 159 2042 Fragments arranged by size: 2042 1785 1071 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2304 2912 3480 4157 5305 5550 1606 Size: 433 265 608 568 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 608 568 433 265 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2889 513 Size: 552 939 885 3688 Fragments arranged by size: 3688 939 885 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 3422 4967 1132 Size: 97 2193 1545 2229 Fragments arranged by size: 2229 2193 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6064 PfoI T'CCnGG_A Cuts at: 2643 3227 2643 Size: 584 5480 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2412 2632 2412 Size: 220 5844 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 2592 3059 3634 2592 Size: 467 575 5022 Fragments arranged by size: 5022 575 467 PspXI vC'TCGA_Gb Cuts at: 2255 2255 Size: 6064 PstI C_TGCA'G Cuts at: 830 3416 830 Size: 2586 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2912 2912 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SfcI C'TryA_G Cuts at: 826 1045 3412 4547 5228 5419 826 Size: 219 2367 1135 681 191 1471 Fragments arranged by size: 2367 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 2824 4168 5069 5331 820 Size: 197 926 312 569 1344 901 262 1553 Fragments arranged by size: 1553 1344 926 901 569 312 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 3422 1132 Size: 2290 3774 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6064 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2328 2511 2682 2889 3503 Size: 552 939 318 6 183 171 207 614 Cuts at: 3503 513 Size: 3074 Fragments arranged by size: 3074 939 614 552 318 207 183 171 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2732 4091 4108 4261 4446 4891 325 Size: 1255 1152 1359 17 153 185 445 1498 Fragments arranged by size: 1498 1359 1255 1152 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4310 6022 96 Size: 274 80 33 51 1443 2333 1712 138 Fragments arranged by size: 2333 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2527 2559 6024 6056 2527 Size: 32 3465 32 2535 Fragments arranged by size: 3465 2535 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 2553 2737 2306 Size: 247 184 5633 Fragments arranged by size: 5633 247 184 XhoI C'TCGA_G Cuts at: 1943 2255 1943 Size: 312 5752 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 3236 4193 3236 Size: 957 5107 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI