(Circular) (Six-base) MAPSORT of: pFN21AB8828.seq Check: 2377 from: 1 to: 6007 pFN21AB8828 6007 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2487 2487 Size: 6007 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2563 3817 278 Size: 53 83 186 936 1027 1254 2468 Fragments arranged by size: 2468 1254 1027 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 2033 3328 2033 Size: 1295 4712 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4070 5454 5722 1632 Size: 2438 1384 268 1917 Fragments arranged by size: 2438 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 3304 4906 5883 1203 Size: 2101 1602 977 1327 Fragments arranged by size: 2101 1602 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6007 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AlwNI CAG_nnn'CTG Cuts at: 2876 2887 3750 5322 2876 Size: 11 863 1572 3561 Fragments arranged by size: 3561 1572 863 11 ApaLI G'TGCA_C Cuts at: 4064 5220 4064 Size: 1156 4851 ApoI r'AATT_y Cuts at: 1493 3316 3542 3694 5803 1493 Size: 1823 226 152 2109 1697 Fragments arranged by size: 2109 1823 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2505 3332 1870 Size: 73 562 827 4545 Fragments arranged by size: 4545 827 562 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 3136 3328 4783 618 Size: 325 217 627 246 1103 192 1455 1842 Fragments arranged by size: 1842 1455 1103 627 325 246 217 192 BanII G_rGCy'C Cuts at: 1391 2534 2861 3016 3326 1391 Size: 1143 327 155 310 4072 Fragments arranged by size: 4072 1143 327 310 155 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2330 928 Size: 971 431 4605 Fragments arranged by size: 4605 971 431 BbvCI CC'TCA_GC Cuts at: 2348 2348 Size: 6007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2625 2659 4198 4232 1903 Size: 34 50 34 604 34 1539 34 3678 Fragments arranged by size: 3678 1539 604 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2087 3194 4618 Size: 107 122 71 631 605 415 1107 1424 Cuts at: 4618 136 Size: 1525 Fragments arranged by size: 1525 1424 1107 631 605 415 122 107 71 BglII A'GATC_T Cuts at: 6002 6002 Size: 6007 BlpI GC'TnA_GC Cuts at: 3424 3424 Size: 6007 Bme1580I G_kGCm'C Cuts at: 1790 2906 4068 5224 1790 Size: 1116 1162 1156 2573 Fragments arranged by size: 2573 1162 1156 1116 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3738 4696 442 Size: 416 53 83 399 24 2321 958 1753 Fragments arranged by size: 2321 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2079 3000 3030 4667 1239 Size: 840 921 30 1637 2579 Fragments arranged by size: 2579 1637 921 840 30 Bpu10I CC'TnA_GC Cuts at: 2348 2744 2855 2348 Size: 396 111 5500 Fragments arranged by size: 5500 396 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2161 2420 4132 4997 5295 2161 Size: 259 1712 865 298 2873 Fragments arranged by size: 2873 1712 865 298 259 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4670 882 Size: 617 3171 2219 Fragments arranged by size: 3171 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3798 493 Size: 711 2594 2702 Fragments arranged by size: 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2560 3814 4196 4831 Size: 53 83 186 936 1027 1254 382 635 Cuts at: 4831 275 Size: 1451 Fragments arranged by size: 1451 1254 1027 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2032 2062 3232 3262 1178 Size: 30 165 30 629 30 1170 30 3923 Fragments arranged by size: 3923 1170 629 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2423 2469 2529 2741 2744 2774 1186 Size: 904 333 46 60 212 3 30 4419 Fragments arranged by size: 4419 904 333 212 60 46 30 3 BseYI C'CCAG_C Cuts at: 2985 5210 5706 2985 Size: 2225 496 3286 Fragments arranged by size: 3286 2225 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2734 2850 3024 1163 Size: 573 998 116 174 4146 Fragments arranged by size: 4146 998 573 174 116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2861 3016 4068 4153 5224 2861 Size: 155 1052 85 1071 3644 Fragments arranged by size: 3644 1071 1052 155 85 BsmI GAATG_Cn' Cuts at: 2740 3518 3611 2740 Size: 778 93 5136 Fragments arranged by size: 5136 778 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2534 2861 2906 3016 3326 4068 4153 Size: 399 744 327 45 110 310 742 85 Cuts at: 4153 5224 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 744 742 399 327 310 110 85 45 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 2563 3896 5626 2563 Size: 1333 1730 2944 Fragments arranged by size: 2944 1730 1333 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 2764 2793 3362 844 Size: 1643 277 29 569 3489 Fragments arranged by size: 3489 1643 569 277 29 BsrBI CCG'CTC Cuts at: 1394 1823 3894 1394 Size: 429 2071 3507 Fragments arranged by size: 3507 2071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2928 4502 4676 65 Size: 1183 1680 1574 174 1396 Fragments arranged by size: 1680 1574 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2383 2631 4651 4838 1700 Size: 110 573 248 2020 187 2869 Fragments arranged by size: 2869 2020 573 248 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 2169 3047 794 Size: 1375 878 3754 Fragments arranged by size: 3754 1375 878 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstXI CCAn_nnnn'nTGG Cuts at: 3253 3253 Size: 6007 BstYI r'GATC_y Cuts at: 1972 3337 3704 4089 4106 5558 5644 5656 6002 Size: 1365 367 385 17 1452 86 12 346 Cuts at: 6002 1972 Size: 1977 Fragments arranged by size: 1977 1452 1365 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2290 2342 2923 3153 3252 513 Size: 552 1225 52 581 230 99 3268 Fragments arranged by size: 3268 1225 581 552 230 99 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 2107 2862 3523 4317 4337 2107 Size: 755 661 794 20 3777 Fragments arranged by size: 3777 794 755 661 20 ClaI AT'CG_AT Cuts at: 1554 3203 1554 Size: 1649 4358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2790 3294 3454 Size: 54 1425 50 275 18 960 504 160 Cuts at: 3454 4343 5972 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 960 889 504 275 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3937 1353 Size: 2584 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2859 3014 2859 Size: 155 5852 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 3000 3030 4070 4667 5454 5722 Size: 393 447 921 30 1040 597 787 268 Cuts at: 5722 1239 Size: 1524 Fragments arranged by size: 1524 1040 921 787 597 447 393 268 30 EcoO109I rG'GnC_Cy Cuts at: 2054 2413 2054 Size: 359 5648 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 EcoRV GAT'ATC Cuts at: 2177 2578 2177 Size: 401 5606 FspI TGC'GCA Cuts at: 3287 4513 3287 Size: 1226 4781 FspAI rTGC'GCAy Cuts at: 3287 3287 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 5154 1678 Size: 3476 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373 Size: 32 135 32 3076 32 42 32 2626 Fragments arranged by size: 3076 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3351 3597 669 Size: 988 281 1413 246 3079 Fragments arranged by size: 3079 1413 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 2037 3332 2037 Size: 1295 4712 MfeI C'AATT_G Cuts at: 3120 3606 3120 Size: 486 5521 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2229 5121 5305 1305 Size: 924 2892 184 2007 Fragments arranged by size: 2892 2007 924 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2792 10 Size: 54 1425 343 960 3225 Fragments arranged by size: 3225 1425 960 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3251 3296 3965 4324 4483 518 Size: 648 2085 45 669 359 159 2042 Fragments arranged by size: 2085 2042 669 648 359 159 45 MspA1I CmG'CkG Cuts at: 1606 2046 2366 3020 3104 3423 4100 5248 5493 Size: 440 320 654 84 319 677 1148 245 Cuts at: 5493 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 654 440 320 319 245 84 NaeI GCC'GGC Cuts at: 2633 2633 Size: 6007 NcoI C'CATG_G Cuts at: 513 1065 2290 2923 3153 3252 513 Size: 552 1225 633 230 99 3268 Fragments arranged by size: 3268 1225 633 552 230 99 NdeI CA'TA_TG Cuts at: 387 2677 387 Size: 2290 3717 NgoMIV G'CCGG_C Cuts at: 2631 2631 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 3286 3365 4910 1132 Size: 97 2057 79 1545 2229 Fragments arranged by size: 2229 2057 1545 97 79 PasI CC'CwG_GG Cuts at: 1348 3127 1348 Size: 1779 4228 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 3133 3217 1108 Size: 2025 84 3898 Fragments arranged by size: 3898 2025 84 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2413 2413 Size: 6007 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PstI C_TGCA'G Cuts at: 830 3359 830 Size: 2529 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2366 3020 3104 2366 Size: 654 84 5269 Fragments arranged by size: 5269 654 84 SacI G_AGCT'C Cuts at: 2861 3016 2861 Size: 155 5852 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2128 2966 3355 4490 5171 5362 826 Size: 219 1083 838 389 1135 681 191 1471 Fragments arranged by size: 1471 1135 1083 838 681 389 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 2631 1700 Size: 931 5076 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2140 2435 4111 5012 5274 820 Size: 197 926 197 295 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 295 262 197 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3286 3365 1132 Size: 2154 79 3774 Fragments arranged by size: 3774 2154 79 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6007 StyI C'CwwG_G Cuts at: 513 1065 2290 2298 2371 2841 2923 3153 3216 Size: 552 1225 8 73 470 82 230 63 Cuts at: 3216 3252 3446 513 Size: 36 194 3074 Fragments arranged by size: 3074 1225 552 470 230 194 82 73 63 36 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2199 4034 4051 4204 4389 4834 325 Size: 1255 619 1835 17 153 185 445 1498 Fragments arranged by size: 1835 1498 1255 619 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4253 5965 96 Size: 274 80 33 51 1443 2276 1712 138 Fragments arranged by size: 2276 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2032 2064 3232 3264 5967 5999 2032 Size: 32 1168 32 2703 32 2040 Fragments arranged by size: 2703 2040 1168 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2559 1316 Size: 1243 4764 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3160 3250 3160 Size: 90 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2561 3815 276 Size: 53 83 186 936 1027 1254 2468 Fragments arranged by size: 2468 1254 1027 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI