Restriction map for
pFN21AB8818
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1286I BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI Hin1I HindIII HpaII KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 97 CCGC CCGC(-3/-1)
AflII 580 CTTAAG C^TTAAG
AflIII 410 ACATGT A^C[GA][CT]GT
AlwNI 39 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 466 ATTAAT AT^TAAT
AsnI 466 ATTAAT AT^TAAT
Asp700I 907 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI 41 GCAGC GCAGC(8/12)
BciVI 1084 GGATAC GTATCC(6/5)
BfrI 580 CTTAAG C^TTAAG
BsaJI 140 CCATGG C^C[ACGT][ACGT]GG
BseRI 54 CTCCTC GAGGAG(10/8)
BsiYI 766 CCTAATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 766 CCTAATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 65 GAATGC GAATGC(1/-1)
Bsp1407I 413 TGTACA T^GTACA
BspLU11I 410 ACATGT A^CATGT
BsrDI 291 CATTGC GCAATG(2/0)
BsrGI 413 TGTACA T^GTACA
BssKI 1081 CCAGG ^CC[ACGT]GG
BstNI 1081 CCAGG CC^[AT]GG
BstYI 966 AGATCC [GA]^GATC[CT]
CfoI 69 GCGC GCG^C
DraI 1075 TTTAAA TTT^AAA
DsaI 140 CCATGG C^C[GA][CT]GG
EcoRII 1081 CCAGG ^CC[AT]GG
EcoT14I 140 CCATGG C^C[AT][AT]GG
Fnu4HI 41 GCAGC GC^[ACGT]GC
HaeIII 47 GGCC GG^CC
HhaI 69 GCGC GCG^C
HinP1I 69 GCGC G^CGC
HincII 168 GTTAAC GT[CT]^[GA]AC
HindII 168 GTTAAC GT[CT]^[GA]AC
HpaI 168 GTTAAC GTT^AAC
ItaI 41 GCAGC GC^[ACGT]GC
MaeII 284 ACGT A^CGT
MflI 966 AGATCC [GA]^GATC[CT]
MspA1I 42 CAGCTG C[AC]G^C[GT]G
MvaI 1081 CCAGG CC^[AT]GG
MwoI 784 GCCACTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 140 CCATGG C^CATGG
NspI 410 ACATGT [GA]CATG^[CT]
PshBI 466 ATTAAT AT^TAAT
PvuII 42 CAGCTG CAG^CTG
ScaI 1138 AGTACT AGT^ACT
ScrFI 1081 CCAGG CC^[ACGT]GG
SfaNI 537 GATGC GCATC(5/9)
SfcI 788 CTACAG C^T[GA][CT]AG
SmlI 580 CTTAAG C^T[CT][GA]AG
SspBI 413 TGTACA T^GTACA
StyI 140 CCATGG C^C[AT][AT]GG
TaqI 855 TCGA T^CGA
TseI 41 GCAGC G^C[AT]GC
TthHB8I 855 TCGA T^CGA
XhoII 966 AGATCC [GA]^GATC[CT]
XmnI 907 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AlwI 967 GATCC GGATC(4/5)
450 GGATC
BfaI 364 CTAG C^TAG
1127 CTAG
BsmFI 201 GGGAC GGGAC(10/14)
137 GTCCC
Cac8I 44 GCTGGC GC[ACGT]^[ACGT]GC
540 GCTTGC
DdeI 111 CTCAG C^T[ACGT]AG
963 CTTAG
DraIII 362 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
603 CACAATGTG
HphI 1042 GGTGA GGTGA(8/7)
246 TCACC
MaeI 364 CTAG C^TAG
1127 CTAG
MslI 15 CATAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
663 CATTCTTGTG
TspRI 395 CACTG CA[GC]TG[ACGT][ACGT]^
1112 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsrI
BstF5I DpnII FokI HinfI MaeIII
MboI MboII MnlI MseI NdeII
NlaIII RsaI Sau3AI TfiI Tru9I
Tsp509I