(Circular) (Six-base) MAPSORT of: pFN21AB8818.seq Check: 1208 from: 1 to: 5848 pFN21AB8818 5848 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2022 3057 3911 5295 5563 1632 Size: 390 1035 854 1384 268 1917 Fragments arranged by size: 1917 1384 1035 854 390 268 AflII C'TTAA_G Cuts at: 820 1017 2585 820 Size: 197 1568 4083 Fragments arranged by size: 4083 1568 197 AflIII A'CryG_T Cuts at: 1203 2415 4747 5724 1203 Size: 1212 2332 977 1327 Fragments arranged by size: 2332 1327 1212 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2450 1184 Size: 1266 4582 AlwNI CAG_nnn'CTG Cuts at: 2049 3591 5163 2049 Size: 1542 1572 2734 Fragments arranged by size: 2734 1572 1542 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2263 2274 2765 3077 3157 3383 3535 5644 Size: 770 11 491 312 80 226 152 2109 Cuts at: 5644 1493 Size: 1697 Fragments arranged by size: 2109 1697 770 491 312 226 152 80 11 AseI AT'TA_AT Cuts at: 160 1032 2472 4402 160 Size: 872 1440 1930 1606 Fragments arranged by size: 1930 1606 1440 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3173 1870 Size: 73 1230 4545 Fragments arranged by size: 4545 1230 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3096 3129 3096 Size: 33 5815 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3169 4624 618 Size: 325 217 627 1382 1455 1842 Fragments arranged by size: 1842 1455 1382 627 325 217 BanII G_rGCy'C Cuts at: 1391 3167 1391 Size: 1776 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3083 3740 4950 3083 Size: 657 1210 3981 Fragments arranged by size: 3981 1210 657 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4459 136 Size: 107 122 71 631 605 2787 1525 Fragments arranged by size: 2787 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 3909 5065 1790 Size: 2119 1156 2573 Fragments arranged by size: 2573 2119 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3579 4537 442 Size: 416 53 83 399 24 2162 958 1753 Fragments arranged by size: 2162 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4508 1239 Size: 3269 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3973 4838 5136 3973 Size: 865 298 4685 Fragments arranged by size: 4685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5623 Fragments arranged by size: 5623 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2050 1186 Size: 864 4984 BseYI C'CCAG_C Cuts at: 5051 5547 5051 Size: 496 5352 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3909 3994 5065 3909 Size: 85 1071 4692 Fragments arranged by size: 4692 1071 85 BsmI GAATG_Cn' Cuts at: 2076 3359 3452 2076 Size: 1283 93 4472 Fragments arranged by size: 4472 1283 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3167 3909 3994 5065 1391 Size: 399 1377 742 85 1071 2174 Fragments arranged by size: 2174 1377 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3203 844 Size: 2359 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 4343 4517 65 Size: 1183 1047 2048 174 1396 Fragments arranged by size: 2048 1396 1183 1047 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4492 4679 1700 Size: 110 2682 187 2869 Fragments arranged by size: 2869 2682 187 110 BsrGI T'GTAC_A Cuts at: 96 2418 96 Size: 2322 3526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2971 3178 3545 3930 3947 5399 5485 5497 Size: 999 207 367 385 17 1452 86 12 Cuts at: 5497 5843 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 999 385 367 346 207 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2145 513 Size: 552 1080 4216 Fragments arranged by size: 4216 1080 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 3123 3364 4158 4178 3123 Size: 241 794 20 4793 Fragments arranged by size: 4793 794 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2342 2377 529 Size: 35 1778 35 4000 Fragments arranged by size: 4000 1778 35 35 DraI TTT'AAA Cuts at: 3082 3152 3082 Size: 70 5778 DraIII CAC_nnn'GTG Cuts at: 2372 2613 2372 Size: 241 5607 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3295 4184 5813 Size: 54 1425 50 275 18 1465 889 1629 Cuts at: 5813 8 Size: 43 Fragments arranged by size: 1629 1465 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3778 1353 Size: 2425 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2022 3057 3911 4508 5295 5563 1239 Size: 393 390 1035 854 597 787 268 1524 Fragments arranged by size: 1524 1035 854 787 597 393 390 268 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 4995 1678 Size: 3317 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2533 2565 4489 4521 4563 Size: 32 135 32 961 32 1924 32 42 Cuts at: 4563 4595 1373 Size: 32 2626 Fragments arranged by size: 2626 1924 961 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2175 3192 3438 669 Size: 988 281 237 1017 246 3079 Fragments arranged by size: 3079 1017 988 281 246 237 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 2175 3438 2175 Size: 1263 4585 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5146 1305 Size: 3657 184 2007 Fragments arranged by size: 3657 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4026 Fragments arranged by size: 4026 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2672 3806 4165 4324 518 Size: 648 858 648 1134 359 159 2042 Fragments arranged by size: 2042 1134 858 648 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 3264 3941 5089 5334 1606 Size: 443 1215 677 1148 245 2120 Fragments arranged by size: 2120 1215 1148 677 443 245 NcoI C'CATG_G Cuts at: 513 1065 2145 513 Size: 552 1080 4216 Fragments arranged by size: 4216 1080 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2419 3206 4751 1132 Size: 97 1190 787 1545 2229 Fragments arranged by size: 2229 1545 1190 787 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 2415 4747 2415 Size: 2332 3516 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 2081 3418 2081 Size: 1337 4511 PstI C_TGCA'G Cuts at: 830 3200 830 Size: 2370 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2049 2049 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 3145 4096 5808 3145 Size: 951 1712 3185 Fragments arranged by size: 3185 1712 951 SfcI C'TryA_G Cuts at: 826 1045 2793 3196 4331 5012 5203 826 Size: 219 1748 403 1135 681 191 1471 Fragments arranged by size: 1748 1471 1135 681 403 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 3952 4853 5115 820 Size: 197 926 642 1367 901 262 1553 Fragments arranged by size: 1553 1367 926 901 642 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2145 3287 513 Size: 552 1080 1142 3074 Fragments arranged by size: 3074 1142 1080 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2466 3875 3892 4045 4230 4675 325 Size: 1255 886 1409 17 153 185 445 1498 Fragments arranged by size: 1498 1409 1255 886 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 2815 3143 Size: 274 80 33 51 1443 441 397 328 Cuts at: 3143 4094 5806 96 Size: 951 1712 138 Fragments arranged by size: 1712 1443 951 441 397 328 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2356 2388 5808 5840 2356 Size: 32 3420 32 2364 Fragments arranged by size: 3420 2364 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 2916 3977 2916 Size: 1061 4787 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI