Restriction map for
pFN21AB8805
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BseAI BsgI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DpnII DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI HindIII HpaI
KpnI MaeI MamI MboI MfeI
MflI MluI MroI MseI MslI
MunI NcoI NdeI NdeII NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SalI Sau3AI SbfI ScaI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 20 enzymes) #
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AgeI 217 ACCGGT A^CCGGT
AlwNI 79 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 939 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 761 TGCGCA TGC^GCA
BalI 246 TGGCCA TGG^CCA
BsaWI 217 ACCGGT [AT]^CCGG[AT]
BsmFI 378 GTCCC GGGAC(10/14)
BstAPI 78 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 601 GACTACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 462 GGCCGGCC GGCCGG^CC
FspI 761 TGCGCA TGC^GCA
MaeIII 221 GTAAC ^GT[ACGT]AC
MluNI 246 TGGCCA TGG^CCA
MscI 246 TGGCCA TGG^CCA
NlaIII 1002 CATG CATG^
PinAI 217 ACCGGT A^CCGGT
PvuII 394 CAGCTG CAG^CTG
SexAI 962 ACCTGGT A^CC[AT]GGT
TspRI 320 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 939 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 697 GTCTAC GT^[AC][GT]AC
1012 GTCTAC
ApaI 315 GGGCCC GGGCC^C
550 GGGCCC
AspHI 304 GAGCTC G[AT]GC[AT]^C
703 GAGCTC
AvaII 596 GGACC G^G[AT]CC
1029 GGACC
BlpI 396 GCTGAGC GC^T[ACGT]AGC
705 GCTCAGC
BpmI 6 CTGGAG CTGGAG(16/14)
187 CTGGAG
Bpu1102I 396 GCTGAGC GC^T[ACGT]AGC
705 GCTCAGC
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
703 GAGCTC
BssSI 276 CTCGTG CACGAG(-5/-1)
921 CTCGTG
CelII 396 GCTGAGC GC^T[ACGT]AGC
705 GCTCAGC
DraII 314 AGGGCCC [GA]G^G[ACGT]CC[CT]
550 GGGCCCT
EagI 461 CGGCCG C^GGCCG
824 CGGCCG
EclXI 461 CGGCCG C^GGCCG
824 CGGCCG
Eco52I 461 CGGCCG C^GGCCG
824 CGGCCG
EcoO109I 314 AGGGCCC [GA]G^G[ACGT]CC[CT]
550 GGGCCCT
HgaI 388 GACGC GACGC(5/10)
851 GCGTC
HinfI 404 GACTC G^A[ACGT]TC
514 GACTC
HphI 860 TCACC GGTGA(8/7)
989 TCACC
KspI 239 CCGCGG CCGC^GG
835 CCGCGG
NaeI 463 GCCGGC GCC^GGC
689 GCCGGC
NgoMIV 463 GCCGGC G^CCGGC
689 GCCGGC
PleI 404 GACTC GAGTC(4/5)
514 GACTC
SacI 304 GAGCTC GAGCT^C
703 GAGCTC
SacII 239 CCGCGG CCGC^GG
835 CCGCGG
SapI 111 GCTCTTC GCTCTTC(1/4)
306 GCTCTTC
SmaI 20 CCCGGG CCC^GGG
210 CCCGGG
TaqI 560 TCGA T^CGA
572 TCGA
TthHB8I 560 TCGA T^CGA
572 TCGA
XmaCI 20 CCCGGG C^CCGGG
210 CCCGGG
XmaI 20 CCCGGG C^CCGGG
210 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HpaII
ItaI KasI Ksp632I MaeII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIV RsaI
Sau96I ScrFI SfaNI SfoI TseI