(Circular) (Six-base) MAPSORT of: pFN21AB8805.seq Check: 7235 from: 1 to: 5758 pFN21AB8805 5758 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2703 3018 3101 1937 Size: 766 315 83 4594 Fragments arranged by size: 4594 766 315 83 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2899 3821 5205 5473 1632 Size: 1267 922 1384 268 1917 Fragments arranged by size: 1917 1384 1267 922 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 2222 4589 2222 Size: 2367 3391 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2126 1184 Size: 942 4816 AlwNI CAG_nnn'CTG Cuts at: 2089 3501 5073 2089 Size: 1412 1572 2774 Fragments arranged by size: 2774 1572 1412 ApaI G_GGCC'C Cuts at: 2324 2559 2324 Size: 235 5523 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 3067 3293 3445 5554 1493 Size: 1574 226 152 2109 1697 Fragments arranged by size: 2109 1697 1574 226 152 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2215 2306 3083 1870 Size: 73 82 190 91 777 4545 Fragments arranged by size: 4545 777 190 91 82 73 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2219 2492 2535 2569 Size: 325 217 627 243 189 273 43 34 Cuts at: 2569 2674 2691 2846 3079 4534 618 Size: 105 17 155 233 1455 1842 Fragments arranged by size: 1842 1455 627 325 273 243 233 217 189 155 105 43 34 17 BanII G_rGCy'C Cuts at: 1391 2313 2324 2559 2712 3077 1391 Size: 922 11 235 153 365 4072 Fragments arranged by size: 4072 922 365 235 153 11 BbeI G_GCGC'C Cuts at: 2034 2496 2678 2695 2850 2034 Size: 462 182 17 155 4942 Fragments arranged by size: 4942 462 182 155 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4787 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2550 2554 2689 Size: 107 122 71 631 605 878 4 135 Cuts at: 2689 4369 136 Size: 1680 1525 Fragments arranged by size: 1680 1525 878 631 605 135 122 107 71 4 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 2402 2711 3175 2402 Size: 309 464 4985 Fragments arranged by size: 4985 464 309 Bme1580I G_kGCm'C Cuts at: 1790 2222 2324 2559 2672 3819 4975 1790 Size: 432 102 235 113 1147 1156 2573 Fragments arranged by size: 2573 1156 1147 432 235 113 102 BmgBI CAC'GTC Cuts at: 1318 2614 1318 Size: 1296 4462 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2318 3489 4447 Size: 416 53 83 399 24 901 1171 958 Cuts at: 4447 442 Size: 1753 Fragments arranged by size: 1753 1171 958 901 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 2213 4418 1239 Size: 793 181 2205 2579 Fragments arranged by size: 2579 2205 793 181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4421 882 Size: 617 2922 2219 Fragments arranged by size: 2922 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2493 2675 2692 Size: 53 83 186 936 498 462 182 17 Cuts at: 2692 2847 3565 3947 4582 275 Size: 155 718 382 635 1451 Fragments arranged by size: 1451 936 718 635 498 462 382 186 182 155 83 53 17 BsaWI w'CCGG_w Cuts at: 2222 4191 4589 4863 5010 5421 2222 Size: 1969 398 274 147 411 2559 Fragments arranged by size: 2559 1969 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2156 2180 2186 2210 1178 Size: 30 165 30 753 24 6 24 4726 Fragments arranged by size: 4726 753 165 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2161 2897 3068 1186 Size: 975 736 171 3876 Fragments arranged by size: 3876 975 736 171 BseYI C'CCAG_C Cuts at: 2414 4961 5457 2414 Size: 2547 496 2715 Fragments arranged by size: 2715 2547 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2469 2581 2832 3208 3969 4997 Size: 275 186 468 112 251 376 761 1028 Cuts at: 4997 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 468 376 275 251 186 112 BsiHKAI G_wGCw'C Cuts at: 2313 2712 3819 3904 4975 2313 Size: 399 1107 85 1071 3096 Fragments arranged by size: 3096 1107 1071 399 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2222 2313 2324 2559 2672 2712 3077 Size: 399 432 91 11 235 113 40 365 Cuts at: 3077 3819 3904 4975 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 365 235 113 91 85 40 11 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3113 844 Size: 2269 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2116 2406 2575 2583 3645 1394 Size: 429 293 290 169 8 1062 3507 Fragments arranged by size: 3507 1062 429 293 290 169 8 BsrDI GCAATG_nn' Cuts at: 65 1248 4253 4427 65 Size: 1183 3005 174 1396 Fragments arranged by size: 3005 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2222 2367 2468 2694 2732 2996 4402 Size: 110 412 145 101 226 38 264 1406 Cuts at: 4402 4589 1700 Size: 187 2869 Fragments arranged by size: 2869 1406 412 264 226 187 145 110 101 38 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 2418 2804 2884 1925 Size: 493 386 80 4799 Fragments arranged by size: 4799 493 386 80 BssSI C'ACGA_G Cuts at: 2281 2926 3818 4830 2281 Size: 645 892 1012 3209 Fragments arranged by size: 3209 1012 892 645 BstAPI GCAn_nnn'nTGC Cuts at: 794 2089 794 Size: 1295 4463 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstYI r'GATC_y Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753 Size: 1116 367 385 17 1452 86 12 346 Cuts at: 5753 1972 Size: 1977 Fragments arranged by size: 1977 1452 1116 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2019 2244 2840 513 Size: 552 954 225 596 3431 Fragments arranged by size: 3431 954 596 552 225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2089 2464 2482 2575 2944 487 Size: 683 56 863 375 18 93 369 3301 Fragments arranged by size: 3301 863 683 375 369 93 56 18 BtsI GCAGTG_nn' Cuts at: 3274 4068 4088 3274 Size: 794 20 4944 Fragments arranged by size: 4944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2612 4765 809 Size: 507 1296 2153 1802 Fragments arranged by size: 2153 1802 1296 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2251 2466 Size: 54 1425 50 275 18 267 154 215 Cuts at: 2466 2696 2829 2896 3205 4094 5723 8 Size: 230 133 67 309 889 1629 43 Fragments arranged by size: 1629 1425 889 309 275 267 230 215 154 133 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2466 2829 3205 1537 Size: 275 654 363 376 4090 Fragments arranged by size: 4090 654 376 363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 2318 2516 3688 1353 Size: 770 195 198 1172 3423 Fragments arranged by size: 3423 1172 770 198 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2801 4345 4719 4865 387 Size: 1064 138 1212 1544 374 146 1280 Fragments arranged by size: 1544 1280 1212 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 2710 2311 Size: 399 5359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2213 2899 3821 4418 5205 5473 Size: 393 400 181 686 922 597 787 268 Cuts at: 5473 1239 Size: 1524 Fragments arranged by size: 1524 922 787 686 597 400 393 268 181 EcoO109I rG'GnC_Cy Cuts at: 2320 2556 2320 Size: 236 5522 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2965 2965 Size: 5758 FseI GG_CCGG'CC Cuts at: 2472 2472 Size: 5758 FspI TGC'GCA Cuts at: 2768 4264 2768 Size: 1496 4262 FspAI rTGC'GCAy Cuts at: 2768 2768 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 2019 2034 2071 2267 2374 2496 2545 2678 Size: 341 15 37 196 107 122 49 133 Cuts at: 2678 2695 2780 2850 4905 1678 Size: 17 85 70 2055 2531 Fragments arranged by size: 2531 2055 341 196 133 122 107 85 70 49 37 17 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2510 2542 4399 4431 4473 Size: 32 135 32 938 32 1857 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 1857 938 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2437 2641 2860 3102 3348 669 Size: 988 281 499 204 219 242 246 3079 Fragments arranged by size: 3079 988 499 281 246 242 219 204 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KasI G'GCGC_C Cuts at: 2030 2492 2674 2691 2846 2030 Size: 462 182 17 155 4942 Fragments arranged by size: 4942 462 182 155 17 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2199 4872 5056 1305 Size: 894 2673 184 2007 Fragments arranged by size: 2673 2007 894 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2253 10 Size: 54 1425 343 421 3515 Fragments arranged by size: 3515 1425 421 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3716 4075 4234 518 Size: 648 2550 359 159 2042 Fragments arranged by size: 2550 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2246 2401 2635 2842 3174 3851 4999 Size: 483 157 155 234 207 332 677 1148 Cuts at: 4999 5244 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 483 332 245 234 207 157 155 NaeI GCC'GGC Cuts at: 2470 2696 2470 Size: 226 5532 NarI GG'CG_CC Cuts at: 2031 2493 2675 2692 2847 2031 Size: 462 182 17 155 4942 Fragments arranged by size: 4942 462 182 155 17 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5206 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NgoMIV G'CCGG_C Cuts at: 2468 2694 2468 Size: 226 5532 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4856 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspOMI G'GGCC_C Cuts at: 2320 2555 2320 Size: 235 5523 PstI C_TGCA'G Cuts at: 830 3110 830 Size: 2280 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2401 2401 Size: 5758 SacI G_AGCT'C Cuts at: 2313 2712 2313 Size: 399 5359 SacII CC_GC'GG Cuts at: 2247 2843 2247 Size: 596 5162 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SapI GCTCTTCn'nnn_ Cuts at: 2123 2318 2123 Size: 195 5563 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SexAI A'CCwGG_T Cuts at: 2967 2967 Size: 5758 SfcI C'TryA_G Cuts at: 826 1045 3106 4241 4922 5113 826 Size: 219 2061 1135 681 191 1471 Fragments arranged by size: 2061 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2032 2494 2676 2693 2848 2032 Size: 462 182 17 155 4942 Fragments arranged by size: 4942 462 182 155 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 2027 2217 3085 1872 Size: 155 190 868 4545 Fragments arranged by size: 4545 868 190 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 3862 4763 5025 820 Size: 197 926 1919 901 262 1553 Fragments arranged by size: 1919 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 3197 513 Size: 552 2132 3074 Fragments arranged by size: 3074 2132 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2618 2924 3785 3802 3955 4140 4585 Size: 1255 1038 306 861 17 153 185 445 Cuts at: 4585 325 Size: 1498 Fragments arranged by size: 1498 1255 1038 861 445 306 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4004 5716 96 Size: 274 80 33 51 1443 2027 1712 138 Fragments arranged by size: 2027 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 2025 2215 3083 1870 Size: 155 190 868 4545 Fragments arranged by size: 4545 868 190 155 XmnI GAAnn'nnTTC Cuts at: 2948 3887 2948 Size: 939 4819 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SfiI SrfI SwaI XcmI