Restriction map for
pFN21AB8805
(Linear) (Six-base) MAPSORT of: KIBB8805.seq Check: 6604 from: 1 to: 1053
KIBB8805.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AccI GT'mk_AC
Cuts at: 0 698 1013 1053
Size: 698 315 40
Fragments arranged by size:
698 315 40
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1053
Size: 894 159
AgeI A'CCGG_T
Cuts at: 0 217 1053
Size: 217 836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 121 1053
Size: 121 932
AlwNI CAG_nnn'CTG
Cuts at: 0 84 1053
Size: 84 969
ApaI G_GGCC'C
Cuts at: 0 319 554 1053
Size: 319 235 499
Fragments arranged by size:
499 319 235
AvaI C'yCGr_G
Cuts at: 0 20 210 301 1053
Size: 20 190 91 752
Fragments arranged by size:
752 190 91 20
BanI G'GyrC_C
Cuts at: 0 25 214 487 530 564 669 686 841
Size: 25 189 273 43 34 105 17 155
Cuts at: 841 1053
Size: 212
Fragments arranged by size:
273 212 189 155 105 43 34 25
17
BanII G_rGCy'C
Cuts at: 0 308 319 554 707 1053
Size: 308 11 235 153 346
Fragments arranged by size:
346 308 235 153 11
BbeI G_GCGC'C
Cuts at: 0 29 491 673 690 845 1053
Size: 29 462 182 17 155 208
Fragments arranged by size:
462 208 182 155 29 17
BglI GCCn_nnn'nGGC
Cuts at: 0 545 549 684 1053
Size: 545 4 135 369
Fragments arranged by size:
545 369 135 4
BlpI GC'TnA_GC
Cuts at: 0 397 706 1053
Size: 397 309 347
Fragments arranged by size:
397 347 309
Bme1580I G_kGCm'C
Cuts at: 0 217 319 554 667 1053
Size: 217 102 235 113 386
Fragments arranged by size:
386 235 217 113 102
BmgBI CAC'GTC
Cuts at: 0 609 1053
Size: 609 444
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 1053
Size: 313 740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 208 1053
Size: 27 181 845
Fragments arranged by size:
845 181 27
BsaHI Gr'CG_yC
Cuts at: 0 26 488 670 687 842 1053
Size: 26 462 182 17 155 211
Fragments arranged by size:
462 211 182 155 26 17
BsaWI w'CCGG_w
Cuts at: 0 217 1053
Size: 217 836
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 151 175 181 205 1053
Size: 151 24 6 24 848
Fragments arranged by size:
848 151 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 892 1053
Size: 156 736 161
Fragments arranged by size:
736 161 156
BseYI C'CCAG_C
Cuts at: 0 409 1053
Size: 409 644
BsiEI CG_ry'CG
Cuts at: 0 464 576 827 1053
Size: 464 112 251 226
Fragments arranged by size:
464 251 226 112
BsiHKAI G_wGCw'C
Cuts at: 0 308 707 1053
Size: 308 399 346
Fragments arranged by size:
399 346 308
Bsp1286I G_dGCh'C
Cuts at: 0 217 308 319 554 667 707 1053
Size: 217 91 11 235 113 40 346
Fragments arranged by size:
346 235 217 113 91 40 11
BsrBI CCG'CTC
Cuts at: 0 111 401 570 578 1053
Size: 111 290 169 8 475
Fragments arranged by size:
475 290 169 111 8
BsrFI r'CCGG_y
Cuts at: 0 217 362 463 689 727 991 1053
Size: 217 145 101 226 38 264 62
Fragments arranged by size:
264 226 217 145 101 62 38
BssHII G'CGCG_C
Cuts at: 0 413 799 879 1053
Size: 413 386 80 174
Fragments arranged by size:
413 386 174 80
BssSI C'ACGA_G
Cuts at: 0 276 921 1053
Size: 276 645 132
Fragments arranged by size:
645 276 132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 1053
Size: 84 969
BtgI C'CryG_G
Cuts at: 0 14 239 835 1053
Size: 14 225 596 218
Fragments arranged by size:
596 225 218 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 84 459 477 570 939 1053
Size: 84 375 18 93 369 114
Fragments arranged by size:
375 369 114 93 84 18
DrdI GACnn_nn'nnGTC
Cuts at: 0 607 1053
Size: 607 446
EaeI y'GGCC_r
Cuts at: 0 92 246 461 691 824 891 1053
Size: 92 154 215 230 133 67 162
Fragments arranged by size:
230 215 162 154 133 92 67
EagI C'GGCC_G
Cuts at: 0 461 824 1053
Size: 461 363 229
Fragments arranged by size:
461 363 229
EarI CTCTTCn'nnn_
Cuts at: 0 118 313 511 1053
Size: 118 195 198 542
Fragments arranged by size:
542 198 195 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 796 1053
Size: 796 257
EcoICRI GAG'CTC
Cuts at: 0 306 705 1053
Size: 306 399 348
Fragments arranged by size:
399 348 306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 208 894 1053
Size: 27 181 686 159
Fragments arranged by size:
686 181 159 27
EcoO109I rG'GnC_Cy
Cuts at: 0 315 551 1053
Size: 315 236 502
Fragments arranged by size:
502 315 236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 960 1053
Size: 960 93
FseI GG_CCGG'CC
Cuts at: 0 467 1053
Size: 467 586
FspI TGC'GCA
Cuts at: 0 763 1053
Size: 763 290
FspAI rTGC'GCAy
Cuts at: 0 763 1053
Size: 763 290
HaeII r_GCGC'y
Cuts at: 0 14 29 66 262 369 491 540 673
Size: 14 15 37 196 107 122 49 133
Cuts at: 673 690 775 845 1053
Size: 17 85 70 208
Fragments arranged by size:
208 196 133 122 107 85 70 49
37 17 15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 1053
Size: 505 32 516
Fragments arranged by size:
516 505 32
HincII GTy'rAC
Cuts at: 0 432 636 855 1053
Size: 432 204 219 198
Fragments arranged by size:
432 219 204 198
KasI G'GCGC_C
Cuts at: 0 25 487 669 686 841 1053
Size: 25 462 182 17 155 212
Fragments arranged by size:
462 212 182 155 25 17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 1053
Size: 194 859
MscI TGG'CCA
Cuts at: 0 248 1053
Size: 248 805
MspA1I CmG'CkG
Cuts at: 0 84 241 396 630 837 1053
Size: 84 157 155 234 207 216
Fragments arranged by size:
234 216 207 157 155 84
NaeI GCC'GGC
Cuts at: 0 465 691 1053
Size: 465 226 362
Fragments arranged by size:
465 362 226
NarI GG'CG_CC
Cuts at: 0 26 488 670 687 842 1053
Size: 26 462 182 17 155 211
Fragments arranged by size:
462 211 182 155 26 17
NgoMIV G'CCGG_C
Cuts at: 0 463 689 1053
Size: 463 226 364
Fragments arranged by size:
463 364 226
PspOMI G'GGCC_C
Cuts at: 0 315 550 1053
Size: 315 235 503
Fragments arranged by size:
503 315 235
PvuII CAG'CTG
Cuts at: 0 396 1053
Size: 396 657
SacI G_AGCT'C
Cuts at: 0 308 707 1053
Size: 308 399 346
Fragments arranged by size:
399 346 308
SacII CC_GC'GG
Cuts at: 0 242 838 1053
Size: 242 596 215
Fragments arranged by size:
596 242 215
SapI GCTCTTCn'nnn_
Cuts at: 0 118 313 1053
Size: 118 195 740
Fragments arranged by size:
740 195 118
SexAI A'CCwGG_T
Cuts at: 0 962 1053
Size: 962 91
SfoI GGC'GCC
Cuts at: 0 27 489 671 688 843 1053
Size: 27 462 182 17 155 210
Fragments arranged by size:
462 210 182 155 27 17
SmaI CCC'GGG
Cuts at: 0 22 212 1053
Size: 22 190 841
Fragments arranged by size:
841 190 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 613 919 1053
Size: 613 306 134
Fragments arranged by size:
613 306 134
XmaI C'CCGG_G
Cuts at: 0 20 210 1053
Size: 20 190 843
Fragments arranged by size:
843 190 20
XmnI GAAnn'nnTTC
Cuts at: 0 943 1053
Size: 943 110
Enzymes that do cut:
AccI AcuI AgeI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BglI BlpI Bme1580I BmgBI BmrI BpmI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BsrBI BsrFI BssHII BssSI BstAPI BtgI BtgZI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI
FseI FspI FspAI HaeII Hin4I HincII KasI MmeI
MscI MspA1I NaeI NarI NgoMIV PspOMI PvuII SacI
SacII SapI SexAI SfoI SmaI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII EcoNI EcoRI EcoRV HindIII
HpaI KpnI MfeI MluI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SalI SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI TstI Tth111I
XbaI XcmI XhoI ZraI