(Circular) (Six-base) MAPSORT of: pFN21AB8796.seq Check: 5341 from: 1 to: 5704 pFN21AB8796 5704 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2565 3047 1937 Size: 628 482 4594 Fragments arranged by size: 4594 628 482 Acc65I G'GTAC_C Cuts at: 2550 3025 2550 Size: 475 5229 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 3767 5151 5419 1632 Size: 407 1728 1384 268 1917 Fragments arranged by size: 1917 1728 1384 407 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 2902 4603 5580 1203 Size: 1699 1701 977 1327 Fragments arranged by size: 1701 1699 1327 977 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5704 AlwNI CAG_nnn'CTG Cuts at: 2752 3447 5019 2752 Size: 695 1572 3437 Fragments arranged by size: 3437 1572 695 ApaLI G'TGCA_C Cuts at: 3761 4917 3761 Size: 1156 4548 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 3029 1870 Size: 73 1086 4545 Fragments arranged by size: 4545 1086 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2550 2661 2938 2972 2990 Size: 325 217 627 763 111 277 34 18 Cuts at: 2990 3025 4480 618 Size: 35 1455 1842 Fragments arranged by size: 1842 1455 763 627 325 277 217 111 35 34 18 BanII G_rGCy'C Cuts at: 1391 2405 3023 1391 Size: 1014 618 4072 Fragments arranged by size: 4072 1014 618 BbeI G_GCGC'C Cuts at: 2665 2994 2665 Size: 329 5375 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2860 928 Size: 971 961 3772 Fragments arranged by size: 3772 971 961 BbvCI CC'TCA_GC Cuts at: 2135 2711 2135 Size: 576 5128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3895 3929 1903 Size: 34 1958 34 3678 Fragments arranged by size: 3678 1958 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2145 4315 136 Size: 107 122 71 631 605 473 2170 1525 Fragments arranged by size: 2170 1525 631 605 473 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 3121 3121 Size: 5704 Bme1580I G_kGCm'C Cuts at: 1790 3765 4921 1790 Size: 1975 1156 2573 Fragments arranged by size: 2573 1975 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2290 3435 4393 Size: 416 53 83 399 24 873 1145 958 Cuts at: 4393 442 Size: 1753 Fragments arranged by size: 1753 1145 958 873 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 3010 2321 Size: 689 5015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 4364 1239 Size: 971 2154 2579 Fragments arranged by size: 2579 2154 971 Bpu10I CC'TnA_GC Cuts at: 2135 2711 2135 Size: 576 5128 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3829 4694 4992 3829 Size: 865 298 4541 Fragments arranged by size: 4541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2547 2620 3495 493 Size: 711 1343 73 875 2702 Fragments arranged by size: 2702 1343 875 711 73 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2662 2991 3511 3893 Size: 53 83 186 936 1129 329 520 382 Cuts at: 3893 4528 275 Size: 635 1451 Fragments arranged by size: 1451 1129 936 635 520 382 329 186 83 53 BsaWI w'CCGG_w Cuts at: 2772 4137 4535 4809 4956 5367 2772 Size: 1365 398 274 147 411 3109 Fragments arranged by size: 3109 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 2892 2922 1178 Size: 30 165 30 1140 30 319 30 3960 Fragments arranged by size: 3960 1140 319 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2306 1186 Size: 937 183 4584 Fragments arranged by size: 4584 937 183 BseYI C'CCAG_C Cuts at: 2286 2293 2715 2942 2947 4907 5403 2286 Size: 7 422 227 5 1960 496 2587 Fragments arranged by size: 2587 1960 496 422 227 7 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3154 3915 4943 1540 Size: 275 186 1153 761 1028 2301 Fragments arranged by size: 2301 1153 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2033 2862 3765 3850 4921 2033 Size: 829 903 85 1071 2816 Fragments arranged by size: 2816 1071 903 829 85 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2405 2862 3023 3765 3850 4921 Size: 399 243 372 457 161 742 85 1071 Cuts at: 4921 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 457 399 372 243 161 85 BspEI T'CCGG_A Cuts at: 2772 5367 2772 Size: 2595 3109 BspHI T'CATG_A Cuts at: 2370 3593 5323 2370 Size: 1223 1730 2751 Fragments arranged by size: 2751 1730 1223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3059 844 Size: 2215 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2646 3591 1394 Size: 429 823 945 3507 Fragments arranged by size: 3507 945 823 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2928 4348 4535 1700 Size: 110 1118 1420 187 2869 Fragments arranged by size: 2869 1420 1118 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 3764 4776 3764 Size: 1012 4692 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5704 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 2449 1184 Size: 1265 4439 BstXI CCAn_nnnn'nTGG Cuts at: 2280 2698 2969 2280 Size: 418 271 5015 Fragments arranged by size: 5015 418 271 BstYI r'GATC_y Cuts at: 1972 2265 2625 3034 3401 3786 3803 5255 5341 Size: 293 360 409 367 385 17 1452 86 Cuts at: 5341 5353 5699 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 409 385 367 360 346 293 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2014 2630 2014 Size: 616 5088 BtgI C'CryG_G Cuts at: 513 1065 2004 2454 513 Size: 552 939 450 3763 Fragments arranged by size: 3763 939 552 450 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 2839 3220 4014 4034 2839 Size: 381 794 20 4509 Fragments arranged by size: 4509 794 381 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DraIII CAC_nnn'GTG Cuts at: 2512 2512 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3151 4040 5669 Size: 54 1425 50 275 18 1321 889 1629 Cuts at: 5669 8 Size: 43 Fragments arranged by size: 1629 1425 1321 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2585 3634 1353 Size: 1232 1049 3423 Fragments arranged by size: 3423 1232 1049 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2210 3767 4364 5151 5419 1239 Size: 393 407 171 1557 597 787 268 1524 Fragments arranged by size: 1557 1524 787 597 407 393 268 171 EcoO109I rG'GnC_Cy Cuts at: 2167 2634 2720 2167 Size: 467 86 5151 Fragments arranged by size: 5151 467 86 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2701 2701 Size: 5704 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 2665 2994 4851 1678 Size: 987 329 1857 2531 Fragments arranged by size: 2531 1857 987 329 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373 Size: 32 135 32 2773 32 42 32 2626 Fragments arranged by size: 2773 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3294 669 Size: 988 281 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KasI G'GCGC_C Cuts at: 2661 2990 2661 Size: 329 5375 KpnI G_GTAC'C Cuts at: 2554 3029 2554 Size: 475 5229 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4818 5002 1305 Size: 3513 184 2007 Fragments arranged by size: 3513 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3882 Fragments arranged by size: 3882 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2696 3662 4021 4180 518 Size: 648 1293 237 966 359 159 2042 Fragments arranged by size: 2042 1293 966 648 359 237 159 MspA1I CmG'CkG Cuts at: 1606 2436 2715 3120 3797 4945 5190 1606 Size: 830 279 405 677 1148 245 2120 Fragments arranged by size: 2120 1148 830 677 405 279 245 NaeI GCC'GGC Cuts at: 2930 2930 Size: 5704 NarI GG'CG_CC Cuts at: 2662 2991 2662 Size: 329 5375 NcoI C'CATG_G Cuts at: 513 1065 2004 2454 513 Size: 552 939 450 3763 Fragments arranged by size: 3763 939 552 450 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NgoMIV G'CCGG_C Cuts at: 2928 2928 Size: 5704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 2906 3062 4607 1132 Size: 97 1677 156 1545 2229 Fragments arranged by size: 2229 1677 1545 156 97 PasI CC'CwG_GG Cuts at: 1348 2596 1348 Size: 1248 4456 PciI A'CATG_T Cuts at: 2902 4603 2902 Size: 1701 4003 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5704 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PmlI CAC'GTG Cuts at: 2547 2547 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2167 2634 2720 2167 Size: 467 86 5151 Fragments arranged by size: 5151 467 86 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PstI C_TGCA'G Cuts at: 830 2748 2937 3056 830 Size: 1918 189 119 3478 Fragments arranged by size: 3478 1918 189 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 PvuII CAG'CTG Cuts at: 2715 2715 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SanDI GG'GwC_CC Cuts at: 2720 2720 Size: 5704 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SexAI A'CCwGG_T Cuts at: 2235 2235 Size: 5704 SfcI C'TryA_G Cuts at: 826 1045 2344 2744 2933 3052 4187 4868 5059 Size: 219 1299 400 189 119 1135 681 191 Cuts at: 5059 826 Size: 1471 Fragments arranged by size: 1471 1299 1135 681 400 219 191 189 119 SfoI GGC'GCC Cuts at: 2663 2992 2663 Size: 329 5375 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3808 4709 4971 820 Size: 197 926 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 2081 1517 Size: 564 5140 StyI C'CwwG_G Cuts at: 513 1065 2004 2454 3143 513 Size: 552 939 450 689 3074 Fragments arranged by size: 3074 939 689 552 450 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2278 2956 3731 3748 3901 4086 4531 Size: 1255 698 678 775 17 153 185 445 Cuts at: 4531 325 Size: 1498 Fragments arranged by size: 1498 1255 775 698 678 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3950 5662 96 Size: 274 80 33 51 1443 1973 1712 138 Fragments arranged by size: 1973 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5664 5696 5664 Size: 32 5672 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI XcmI