Restriction map for
pFN21AB8791
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BfaI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TfiI
Tsp45I TthHB8I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcsI 880 GAATTC [GA]^AATT[CT]
AflII 145 CTTAAG C^TTAAG
AgeI 353 ACCGGT A^CCGGT
Alw44I 279 GTGCAC G^TGCAC
AlwI 419 GGATC GGATC(4/5)
AlwNI 745 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 899 GGGCCC GGGCC^C
ApaLI 279 GTGCAC G^TGCAC
ApoI 880 GAATTC [GA]^AATT[CT]
AspI 37 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 950 CCCGGG C^[CT]CG[GA]G
BanII 899 GGGCCC G[GA]GC[CT]^C
BcgI 162 CGATTATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 188 TGATCA T^GATCA
BfrI 145 CTTAAG C^TTAAG
BglI 688 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 410 AGATCT A^GATCT
BseRI 106 CTCCTC GAGGAG(10/8)
BsoBI 950 CCCGGG C^[CT]CG[GA]G
BsrBI 376 CCGCTC CCGCTC(-3/-3)
CfoI 533 GCGC GCG^C
EcoNI 246 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 880 GAATTC G^AATTC
FbaI 188 TGATCA T^GATCA
HaeII 532 AGCGCC [GA]GCGC^[CT]
HgaI 323 GCGTC GACGC(5/10)
HhaI 533 GCGC GCG^C
HinP1I 533 GCGC G^CGC
HinfI 3 GAGTC G^A[ACGT]TC
MaeIII 371 GTTAC ^GT[ACGT]AC
MfeI 811 CAATTG C^AATTG
MseI 146 TTAA T^TAA
MunI 811 CAATTG C^AATTG
PflFI 37 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 353 ACCGGT A^CCGGT
PleI 3 GAGTC GAGTC(4/5)
PpuMI 437 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 922 CAGCTG CAG^CTG
SbfI 930 CCTGCAGG CCTGCA^GG
SmaI 950 CCCGGG CCC^GGG
SmlI 145 CTTAAG C^T[CT][GA]AG
Sse8387I 930 CCTGCAGG CCTGCA^GG
Tru9I 146 TTAA T^TAA
TspRI 18 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 37 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 950 CCCGGG C^CCGGG
XmaI 950 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 256 GTAC GT^AC
315 GTAC
AflIII 497 ACATGT A^C[GA][CT]GT
512 ACATGT
AspHI 220 GTGCTC G[AT]GC[AT]^C
279 GTGCAC
BalI 119 TGGCCA TGG^CCA
362 TGGCCA
BcnI 950 CCCGG CC^[GC]GG
951 CCGGG
BsaI 635 GAGACC GGTCTC(1/5)
708 GGTCTC
BsiHKAI 220 GTGCTC G[AT]GC[AT]^C
279 GTGCAC
BsmAI 635 GAGAC GTCTC(1/5)
709 GTCTC
BspLU11I 497 ACATGT A^CATGT
512 ACATGT
BspMI 113 ACCTGC ACCTGC(4/8)
209 ACCTGC
BsrFI 353 ACCGGT [GA]^CCGG[CT]
905 ACCGGC
BstYI 410 AGATCT [GA]^GATC[CT]
419 GGATCT
Cfr10I 353 ACCGGT [GA]^CCGG[CT]
905 ACCGGC
DraII 437 GGGTCCT [GA]G^G[ACGT]CC[CT]
898 AGGGCCC
EaeI 119 TGGCCA [CT]^GGCC[GA]
362 TGGCCA
EcoO109I 437 GGGTCCT [GA]G^G[ACGT]CC[CT]
898 AGGGCCC
FauI 375 CCCGC CCCGC(4/6)
549 CCCGC
HincII 43 GTCAAC GT[CT]^[GA]AC
835 GTCAAC
HindII 43 GTCAAC GT[CT]^[GA]AC
835 GTCAAC
MflI 410 AGATCT [GA]^GATC[CT]
419 GGATCT
MluNI 119 TGGCCA TGG^CCA
362 TGGCCA
MscI 119 TGGCCA TGG^CCA
362 TGGCCA
MspA1I 550 CCGCTG C[AC]G^C[GT]G
922 CAGCTG
NciI 950 CCCGG CC^[GC]GG
951 CCGGG
PstI 742 CTGCAG CTGCA^G
931 CTGCAG
RsaI 256 GTAC GT^AC
315 GTAC
Tsp509I 812 AATT ^AATT
881 AATT
XhoII 410 AGATCT [GA]^GATC[CT]
419 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AvaII BbsI BbvI
BmyI BpmI BpuAI BsaJI BsaWI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MboI MboII MnlI
MslI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI XcmI