Restriction map for
pFN21AB8791
(Linear) (Six-base) MAPSORT of: KIBB8791.seq Check: 5949 from: 1 to: 957
KIBB8791.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 957
Size: 117 840
AflII C'TTAA_G
Cuts at: 0 145 957
Size: 145 812
AflIII A'CryG_T
Cuts at: 0 497 512 957
Size: 497 15 445
Fragments arranged by size:
497 445 15
AgeI A'CCGG_T
Cuts at: 0 353 957
Size: 353 604
AleI CACnn'nnGTG
Cuts at: 0 286 957
Size: 286 671
AlwNI CAG_nnn'CTG
Cuts at: 0 750 957
Size: 750 207
ApaI G_GGCC'C
Cuts at: 0 903 957
Size: 903 54
ApaLI G'TGCA_C
Cuts at: 0 279 957
Size: 279 678
ApoI r'AATT_y
Cuts at: 0 880 957
Size: 880 77
AvaI C'yCGr_G
Cuts at: 0 950 957
Size: 950 7
BanII G_rGCy'C
Cuts at: 0 903 957
Size: 903 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 534 693 957
Size: 318 216 159 264
Fragments arranged by size:
318 264 216 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 151 185 957
Size: 151 34 772
Fragments arranged by size:
772 151 34
BclI T'GATC_A
Cuts at: 0 188 957
Size: 188 769
BglI GCCn_nnn'nGGC
Cuts at: 0 694 957
Size: 694 263
BglII A'GATC_T
Cuts at: 0 410 957
Size: 410 547
Bme1580I G_kGCm'C
Cuts at: 0 283 903 957
Size: 283 620 54
Fragments arranged by size:
620 283 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 660 715 957
Size: 660 55 242
Fragments arranged by size:
660 242 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 574 588 957
Size: 549 25 14 369
Fragments arranged by size:
549 369 25 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 629 714 957
Size: 629 85 243
Fragments arranged by size:
629 243 85
BsaWI w'CCGG_w
Cuts at: 0 353 395 451 957
Size: 353 42 56 506
Fragments arranged by size:
506 353 56 42
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 957
Size: 97 860
BseYI C'CCAG_C
Cuts at: 0 624 957
Size: 624 333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 676 798 957
Size: 411 265 122 159
Fragments arranged by size:
411 265 159 122
BsiHKAI G_wGCw'C
Cuts at: 0 224 283 957
Size: 224 59 674
Fragments arranged by size:
674 224 59
Bsp1286I G_dGCh'C
Cuts at: 0 224 283 903 957
Size: 224 59 620 54
Fragments arranged by size:
620 224 59 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 218 957
Size: 122 96 739
Fragments arranged by size:
739 122 96
BsrBI CCG'CTC
Cuts at: 0 378 957
Size: 378 579
BsrFI r'CCGG_y
Cuts at: 0 353 905 957
Size: 353 552 52
Fragments arranged by size:
552 353 52
BstYI r'GATC_y
Cuts at: 0 410 419 957
Size: 410 9 538
Fragments arranged by size:
538 410 9
BtgI C'CryG_G
Cuts at: 0 126 477 578 957
Size: 126 351 101 379
Fragments arranged by size:
379 351 126 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 666 957
Size: 666 291
EaeI y'GGCC_r
Cuts at: 0 119 362 957
Size: 119 243 595
Fragments arranged by size:
595 243 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 549 574 588 957
Size: 117 432 25 14 369
Fragments arranged by size:
432 369 117 25 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 957
Size: 250 707
EcoO109I rG'GnC_Cy
Cuts at: 0 438 899 957
Size: 438 461 58
Fragments arranged by size:
461 438 58
EcoRI G'AATT_C
Cuts at: 0 880 957
Size: 880 77
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 957
Size: 561 396
HaeII r_GCGC'y
Cuts at: 0 536 957
Size: 536 421
HincII GTy'rAC
Cuts at: 0 45 837 957
Size: 45 792 120
Fragments arranged by size:
792 120 45
MfeI C'AATT_G
Cuts at: 0 811 957
Size: 811 146
MscI TGG'CCA
Cuts at: 0 121 364 957
Size: 121 243 593
Fragments arranged by size:
593 243 121
MslI CAynn'nnrTG
Cuts at: 0 184 286 346 957
Size: 184 102 60 611
Fragments arranged by size:
611 184 102 60
MspA1I CmG'CkG
Cuts at: 0 552 924 957
Size: 552 372 33
Fragments arranged by size:
552 372 33
NcoI C'CATG_G
Cuts at: 0 126 477 578 957
Size: 126 351 101 379
Fragments arranged by size:
379 351 126 101
NspI r_CATG'y
Cuts at: 0 315 501 516 957
Size: 315 186 15 441
Fragments arranged by size:
441 315 186 15
PciI A'CATG_T
Cuts at: 0 497 512 957
Size: 497 15 445
Fragments arranged by size:
497 445 15
PfoI T'CCnGG_A
Cuts at: 0 604 957
Size: 604 353
PpuMI rG'GwC_Cy
Cuts at: 0 438 957
Size: 438 519
PspOMI G'GGCC_C
Cuts at: 0 899 957
Size: 899 58
PstI C_TGCA'G
Cuts at: 0 746 935 957
Size: 746 189 22
Fragments arranged by size:
746 189 22
PvuII CAG'CTG
Cuts at: 0 924 957
Size: 924 33
SbfI CC_TGCA'GG
Cuts at: 0 935 957
Size: 935 22
SfcI C'TryA_G
Cuts at: 0 64 468 675 742 931 957
Size: 64 404 207 67 189 26
Fragments arranged by size:
404 207 189 67 64 26
SmaI CCC'GGG
Cuts at: 0 952 957
Size: 952 5
SmlI C'TyrA_G
Cuts at: 0 145 957
Size: 145 812
StyI C'CwwG_G
Cuts at: 0 126 477 536 578 957
Size: 126 351 59 42 379
Fragments arranged by size:
379 351 126 59 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 658 957
Size: 642 16 299
Fragments arranged by size:
642 299 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 895 927 957
Size: 382 32 481 32 30
Fragments arranged by size:
481 382 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 0 40 957
Size: 40 917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 438 585 921 957
Size: 93 345 147 336 36
Fragments arranged by size:
345 336 147 93 36
XmaI C'CCGG_G
Cuts at: 0 950 957
Size: 950 7
Enzymes that do cut:
AcuI AflII AflIII AgeI AleI AlwNI ApaI ApaLI
ApoI AvaI BanII BbsI BcgI BclI BglI BglII
Bme1580I BmrI BpmI BsaI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI BtgZI
EaeI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII HincII
MfeI MscI MslI MspA1I NcoI NspI PciI PfoI
PpuMI PspOMI PstI PvuII SbfI SfcI SmaI SmlI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BciVI BfrBI BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI
KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XhoI XmnI ZraI