Restriction map for
pFN21AB8783
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BfaI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI Bsp1407I BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI TspRI
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 277 GTCTAC GT^[AC][GT]AC
AcsI 824 AAATTT [GA]^AATT[CT]
AflII 651 CTTAAG C^TTAAG
Alw44I 257 GTGCAC G^TGCAC
AlwNI 753 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 257 GTGCAC G^TGCAC
ApoI 824 AAATTT [GA]^AATT[CT]
AspHI 257 GTGCAC G[AT]GC[AT]^C
BalI 509 TGGCCA TGG^CCA
BanI 405 GGTGCC G^G[CT][GA]CC
BanII 789 GAGCCC G[GA]GC[CT]^C
BciVI 204 GGATAC GTATCC(6/5)
BfrI 651 CTTAAG C^TTAAG
BpmI 91 CTGGAG CTGGAG(16/14)
BsiHKAI 257 GTGCAC G[AT]GC[AT]^C
BspDI 317 ATCGAT AT^CGAT
BspMI 402 GCAGGT ACCTGC(4/8)
BsrDI 285 CATTGC GCAATG(2/0)
BsrI 644 CCAGT ACTGG(1/-1)
BssSI 251 CTCGTG CACGAG(-5/-1)
BstAPI 752 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 164 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 86 AGATCC [GA]^GATC[CT]
ClaI 317 ATCGAT AT^CGAT
DraII 686 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 677 CCATGG C^C[GA][CT]GG
EcoO109I 686 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 677 CCATGG C^C[AT][AT]GG
HapII 10 CCGG C^CGG
HpaII 10 CCGG C^CGG
MaeIII 448 GTGAC ^GT[ACGT]AC
MflI 86 AGATCC [GA]^GATC[CT]
MluNI 509 TGGCCA TGG^CCA
MscI 509 TGGCCA TGG^CCA
MspI 10 CCGG C^CGG
NcoI 677 CCATGG C^CATGG
NlaIV 405 GGTGCC GG[ACGT]^[ACGT]CC
NspI 342 ACATGC [GA]CATG^[CT]
PstI 100 CTGCAG CTGCA^G
SbfI 99 CCTGCAGG CCTGCA^GG
SfcI 100 CTGCAG C^T[GA][CT]AG
SmaI 9 CCCGGG CCC^GGG
Sse8387I 99 CCTGCAGG CCTGCA^GG
StyI 677 CCATGG C^C[AT][AT]GG
TfiI 375 GAATC G^A[AT]TC
Tsp45I 448 GTGAC ^GT[GC]AC
XcmI 161 CCACCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 86 AGATCC [GA]^GATC[CT]
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BcnI 9 CCCGG CC^[GC]GG
10 CCGGG
BsmI 693 GAATGC GAATGC(1/-1)
391 GCATTC
BsrBI 240 CCGCTC CCGCTC(-3/-3)
637 CCGCTC
CfoI 5 GCGC GCG^C
438 GCGC
Cfr13I 420 GGCCC G^G[ACGT]CC
687 GGGCC
EaeI 177 CGGCCA [CT]^GGCC[GA]
509 TGGCCA
FauI 636 CCCGC CCCGC(4/6)
183 GCGGG
HaeII 4 AGCGCC [GA]GCGC^[CT]
437 AGCGCC
HhaI 5 GCGC GCG^C
438 GCGC
HinP1I 5 GCGC G^CGC
438 GCGC
HphI 186 GGTGA GGTGA(8/7)
728 TCACC
MaeII 71 ACGT A^CGT
208 ACGT
MboII 221 GAAGA GAAGA(8/7)
330 GAAGA
MseI 326 TTAA T^TAA
652 TTAA
MslI 594 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
609 CACACCTGTG
NciI 9 CCCGG CC^[GC]GG
10 CCGGG
NlaIII 343 CATG CATG^
678 CATG
PleI 118 GAGTC GAGTC(4/5)
493 GAGTC
PvuII 113 CAGCTG CAG^CTG
816 CAGCTG
Sau96I 420 GGCCC G^G[ACGT]CC
687 GGGCC
SfaNI 705 GATGC GCATC(5/9)
618 GCATC
Tru9I 326 TTAA T^TAA
652 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BbvI BmyI BsaJI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MboI MnlI MspA1I MvaI
MwoI NdeII RsaI Sau3AI ScrFI
SmlI StuI TaqI TseI Tsp509I
TthHB8I