(Circular) (Six-base) MAPSORT of: pFN21AB8783.seq Check: 3326 from: 1 to: 5536 pFN21AB8783 5536 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2398 2398 Size: 5536 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3346 278 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2283 2879 1937 Size: 346 596 4594 Fragments arranged by size: 4594 596 346 Acc65I G'GTAC_C Cuts at: 2857 2857 Size: 5536 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 3599 4983 5251 1632 Size: 587 1380 1384 268 1917 Fragments arranged by size: 1917 1384 1380 587 268 AflII C'TTAA_G Cuts at: 820 1017 2656 820 Size: 197 1639 3700 Fragments arranged by size: 3700 1639 197 AflIII A'CryG_T Cuts at: 1203 4435 5412 1203 Size: 3232 977 1327 Fragments arranged by size: 3232 1327 977 AgeI A'CCGG_T Cuts at: 4367 4367 Size: 5536 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 2603 2618 1166 Size: 1437 15 4084 Fragments arranged by size: 4084 1437 15 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2130 2636 1184 Size: 946 506 4084 Fragments arranged by size: 4084 946 506 AlwNI CAG_nnn'CTG Cuts at: 2763 3279 4851 2763 Size: 516 1572 3448 Fragments arranged by size: 3448 1572 516 ApaLI G'TGCA_C Cuts at: 2262 3593 4749 2262 Size: 1331 1156 3049 Fragments arranged by size: 3049 1331 1156 ApoI r'AATT_y Cuts at: 1493 2829 2845 3071 3223 5332 1493 Size: 1336 16 226 152 2109 1697 Fragments arranged by size: 2109 1697 1336 226 152 16 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2141 2552 2861 1870 Size: 73 71 127 411 309 4545 Fragments arranged by size: 4545 411 309 127 73 71 BamHI G'GATC_C Cuts at: 2866 3233 2866 Size: 367 5169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2410 2857 4312 618 Size: 325 217 627 623 447 1455 1842 Fragments arranged by size: 1842 1455 627 623 447 325 217 BanII G_rGCy'C Cuts at: 1391 2798 2855 1391 Size: 1407 57 4072 Fragments arranged by size: 4072 1407 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3727 3761 1903 Size: 34 1790 34 3678 Fragments arranged by size: 3678 1790 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2203 3428 4638 2203 Size: 1225 1210 3101 Fragments arranged by size: 3101 1225 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4147 136 Size: 107 122 71 631 605 2475 1525 Fragments arranged by size: 2475 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5531 5531 Size: 5536 BlpI GC'TnA_GC Cuts at: 2953 2953 Size: 5536 Bme1580I G_kGCm'C Cuts at: 1790 2266 2415 3597 4753 1790 Size: 476 149 1182 1156 2573 Fragments arranged by size: 2573 1182 1156 476 149 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5536 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2643 3267 4225 Size: 416 53 83 399 24 1226 624 958 Cuts at: 4225 442 Size: 1753 Fragments arranged by size: 1753 1226 958 624 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 4196 1239 Size: 878 2079 2579 Fragments arranged by size: 2579 2079 878 Bpu10I CC'TnA_GC Cuts at: 2688 2688 Size: 5536 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2033 2654 3661 4526 4824 2033 Size: 621 1007 865 298 2745 Fragments arranged by size: 2745 1007 865 621 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4199 882 Size: 617 2700 2219 Fragments arranged by size: 2700 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3327 493 Size: 711 2123 2702 Fragments arranged by size: 2702 2123 711 BsaBI GATnn'nnATC Cuts at: 5530 5530 Size: 5536 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3343 3725 4360 275 Size: 53 83 186 936 1810 382 635 1451 Fragments arranged by size: 1810 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3969 4367 4641 4788 5199 3969 Size: 398 274 147 411 4306 Fragments arranged by size: 4306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5311 Fragments arranged by size: 5311 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5536 BseYI C'CCAG_C Cuts at: 4739 5235 4739 Size: 496 5040 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2136 2596 2794 1163 Size: 573 400 460 198 3905 Fragments arranged by size: 3905 573 460 400 198 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2986 3747 4775 1540 Size: 275 186 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2266 3597 3682 4753 2266 Size: 1331 85 1071 3049 Fragments arranged by size: 3049 1331 1071 85 BsmI GAATG_Cn' Cuts at: 2396 2704 3047 3140 2396 Size: 308 343 93 4792 Fragments arranged by size: 4792 343 308 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2415 2798 2855 3597 3682 4753 Size: 399 476 149 383 57 742 85 1071 Cuts at: 4753 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 476 399 383 149 85 57 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 3425 5155 3425 Size: 1730 3806 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2398 2891 844 Size: 1554 493 3489 Fragments arranged by size: 3489 1554 493 BsrBI CCG'CTC Cuts at: 1394 1823 2247 2644 3423 1394 Size: 429 424 397 779 3507 Fragments arranged by size: 3507 779 429 424 397 BsrDI GCAATG_nn' Cuts at: 65 1248 2289 4031 4205 65 Size: 1183 1041 1742 174 1396 Fragments arranged by size: 1742 1396 1183 1041 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4180 4367 1700 Size: 110 2370 187 2869 Fragments arranged by size: 2869 2370 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5536 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5536 BssSI C'ACGA_G Cuts at: 2256 3596 4608 2256 Size: 1340 1012 3184 Fragments arranged by size: 3184 1340 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2763 794 Size: 1969 3567 BstBI TT'CG_AA Cuts at: 4340 4340 Size: 5536 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5536 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2176 Size: 5536 BstYI r'GATC_y Cuts at: 1972 2091 2866 3233 3618 3635 5087 5173 5185 Size: 119 775 367 385 17 1452 86 12 Cuts at: 5185 5531 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 775 385 367 346 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2682 513 Size: 552 1617 3367 Fragments arranged by size: 3367 1617 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4797 Fragments arranged by size: 4797 683 56 BtsI GCAGTG_nn' Cuts at: 3052 3846 3866 3052 Size: 794 20 4722 Fragments arranged by size: 4722 794 20 ClaI AT'CG_AT Cuts at: 1554 2323 1554 Size: 769 4767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5501 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4543 809 Size: 507 3227 1802 Fragments arranged by size: 3227 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2182 2514 2983 Size: 54 1425 50 275 18 352 332 469 Cuts at: 2983 3872 5501 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 469 352 332 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2983 1537 Size: 275 1171 4090 Fragments arranged by size: 4090 1171 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3466 1353 Size: 2113 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4123 4497 4643 387 Size: 1064 138 2534 374 146 1280 Fragments arranged by size: 2534 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2219 3599 4196 4983 5251 1239 Size: 393 485 102 1380 597 787 268 1524 Fragments arranged by size: 1524 1380 787 597 485 393 268 102 EcoO109I rG'GnC_Cy Cuts at: 2692 2692 Size: 5536 EcoRI G'AATT_C Cuts at: 2845 3223 2845 Size: 378 5158 FspI TGC'GCA Cuts at: 4042 4042 Size: 5536 HaeII r_GCGC'y Cuts at: 1678 2013 2446 4683 1678 Size: 335 433 2237 2531 Fragments arranged by size: 2531 2237 433 335 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373 Size: 32 135 32 2605 32 42 32 2626 Fragments arranged by size: 2626 2605 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2880 3126 669 Size: 988 281 942 246 3079 Fragments arranged by size: 3079 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KpnI G_GTAC'C Cuts at: 2861 2861 Size: 5536 MfeI C'AATT_G Cuts at: 3135 3135 Size: 5536 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4650 4834 1305 Size: 3345 184 2007 Fragments arranged by size: 3345 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2516 10 Size: 54 1425 343 684 3030 Fragments arranged by size: 3030 1425 684 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 2618 3494 3853 4012 518 Size: 648 1437 15 876 359 159 2042 Fragments arranged by size: 2042 1437 876 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2120 2188 2301 2823 2952 3629 4777 5022 Size: 514 68 113 522 129 677 1148 245 Cuts at: 5022 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 522 514 245 129 113 68 NcoI C'CATG_G Cuts at: 513 1065 2682 513 Size: 552 1617 3367 Fragments arranged by size: 3367 1617 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5536 NotI GC'GGCC_GC Cuts at: 2983 2983 Size: 5536 NspI r_CATG'y Cuts at: 1132 1229 2351 2894 4439 1132 Size: 97 1122 543 1545 2229 Fragments arranged by size: 2229 1545 1122 543 97 PasI CC'CwG_GG Cuts at: 1348 2415 1348 Size: 1067 4469 PciI A'CATG_T Cuts at: 4435 4435 Size: 5536 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5536 PfoI T'CCnGG_A Cuts at: 2094 2094 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5536 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PstI C_TGCA'G Cuts at: 830 2109 2888 830 Size: 1279 779 3478 Fragments arranged by size: 3478 1279 779 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5536 PvuII CAG'CTG Cuts at: 2120 2823 2120 Size: 703 4833 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SbfI CC_TGCA'GG Cuts at: 2109 2888 2109 Size: 779 4757 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SfcI C'TryA_G Cuts at: 826 1045 2105 2884 4019 4700 4891 826 Size: 219 1060 779 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 779 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5536 SmaI CCC'GGG Cuts at: 1872 2016 2863 1872 Size: 144 847 4545 Fragments arranged by size: 4545 847 144 SmlI C'TyrA_G Cuts at: 820 1017 1943 2048 2656 2669 3640 4541 4803 Size: 197 926 105 608 13 971 901 262 Cuts at: 4803 820 Size: 1553 Fragments arranged by size: 1553 971 926 901 608 262 197 105 13 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5536 SphI G_CATG'C Cuts at: 1132 2894 1132 Size: 1762 3774 SspI AAT'ATT Cuts at: 5 52 3460 5 Size: 47 3408 2081 Fragments arranged by size: 3408 2081 47 StuI AGG'CCT Cuts at: 1517 2033 2432 2718 1517 Size: 516 399 286 4335 Fragments arranged by size: 4335 516 399 286 StyI C'CwwG_G Cuts at: 513 1065 2682 2975 513 Size: 552 1617 293 3074 Fragments arranged by size: 3074 1617 552 293 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2438 2815 3563 3580 3733 3918 4363 Size: 1255 858 377 748 17 153 185 445 Cuts at: 4363 325 Size: 1498 Fragments arranged by size: 1498 1255 858 748 445 377 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3782 5494 96 Size: 274 80 33 51 1443 1805 1712 138 Fragments arranged by size: 1805 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2657 2689 5496 5528 2657 Size: 32 2807 32 2665 Fragments arranged by size: 2807 2665 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2872 2872 Size: 5536 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2173 2173 Size: 5536 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5536 XmaI C'CCGG_G Cuts at: 1870 2014 2861 1870 Size: 144 847 4545 Fragments arranged by size: 4545 847 144 XmnI GAAnn'nnTTC Cuts at: 3665 3665 Size: 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3344 276 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI