Restriction map for
pFN21AB8783
(Linear) (Six-base) MAPSORT of: KIBB8783.seq Check: 8737 from: 1 to: 831
KIBB8783.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 393 831
Size: 393 438
AccI GT'mk_AC
Cuts at: 0 278 831
Size: 278 553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 831
Size: 214 617
AflII C'TTAA_G
Cuts at: 0 651 831
Size: 651 180
AleI CACnn'nnGTG
Cuts at: 0 598 613 831
Size: 598 15 218
Fragments arranged by size:
598 218 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 125 631 831
Size: 125 506 200
Fragments arranged by size:
506 200 125
AlwNI CAG_nnn'CTG
Cuts at: 0 758 831
Size: 758 73
ApaLI G'TGCA_C
Cuts at: 0 257 831
Size: 257 574
ApoI r'AATT_y
Cuts at: 0 824 831
Size: 824 7
AvaI C'yCGr_G
Cuts at: 0 9 136 547 831
Size: 9 127 411 284
Fragments arranged by size:
411 284 127 9
BanI G'GyrC_C
Cuts at: 0 405 831
Size: 405 426
BanII G_rGCy'C
Cuts at: 0 793 831
Size: 793 38
BciVI GTATCCnnnnn_n'
Cuts at: 0 198 831
Size: 198 633
Bme1580I G_kGCm'C
Cuts at: 0 261 410 831
Size: 261 149 421
Fragments arranged by size:
421 261 149
BmrI ACTGGGnnnn_n'
Cuts at: 0 638 831
Size: 638 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 831
Size: 112 719
Bpu10I CC'TnA_GC
Cuts at: 0 683 831
Size: 683 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 649 831
Size: 28 621 182
Fragments arranged by size:
621 182 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 591 789 831
Size: 131 460 198 42
Fragments arranged by size:
460 198 131 42
BsiHKAI G_wGCw'C
Cuts at: 0 261 831
Size: 261 570
BsmI GAATG_Cn'
Cuts at: 0 391 699 831
Size: 391 308 132
Fragments arranged by size:
391 308 132
Bsp1286I G_dGCh'C
Cuts at: 0 261 410 793 831
Size: 261 149 383 38
Fragments arranged by size:
383 261 149 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 393 831
Size: 393 438
BsrBI CCG'CTC
Cuts at: 0 242 639 831
Size: 242 397 192
Fragments arranged by size:
397 242 192
BsrDI GCAATG_nn'
Cuts at: 0 284 831
Size: 284 547
BssSI C'ACGA_G
Cuts at: 0 251 831
Size: 251 580
BstAPI GCAn_nnn'nTGC
Cuts at: 0 758 831
Size: 758 73
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 831
Size: 171 660
BstYI r'GATC_y
Cuts at: 0 86 831
Size: 86 745
BtgI C'CryG_G
Cuts at: 0 677 831
Size: 677 154
ClaI AT'CG_AT
Cuts at: 0 318 831
Size: 318 513
EaeI y'GGCC_r
Cuts at: 0 177 509 831
Size: 177 332 322
Fragments arranged by size:
332 322 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 214 831
Size: 112 102 617
Fragments arranged by size:
617 112 102
EcoO109I rG'GnC_Cy
Cuts at: 0 687 831
Size: 687 144
HaeII r_GCGC'y
Cuts at: 0 8 441 831
Size: 8 433 390
Fragments arranged by size:
433 390 8
MscI TGG'CCA
Cuts at: 0 511 831
Size: 511 320
MslI CAynn'nnrTG
Cuts at: 0 598 613 831
Size: 598 15 218
Fragments arranged by size:
598 218 15
MspA1I CmG'CkG
Cuts at: 0 115 183 296 818 831
Size: 115 68 113 522 13
Fragments arranged by size:
522 115 113 68 13
NcoI C'CATG_G
Cuts at: 0 677 831
Size: 677 154
NspI r_CATG'y
Cuts at: 0 346 831
Size: 346 485
PasI CC'CwG_GG
Cuts at: 0 410 831
Size: 410 421
PfoI T'CCnGG_A
Cuts at: 0 89 831
Size: 89 742
PstI C_TGCA'G
Cuts at: 0 104 831
Size: 104 727
PvuII CAG'CTG
Cuts at: 0 115 818 831
Size: 115 703 13
Fragments arranged by size:
703 115 13
SbfI CC_TGCA'GG
Cuts at: 0 104 831
Size: 104 727
SfcI C'TryA_G
Cuts at: 0 100 831
Size: 100 731
SmaI CCC'GGG
Cuts at: 0 11 831
Size: 11 820
SmlI C'TyrA_G
Cuts at: 0 43 651 664 831
Size: 43 608 13 167
Fragments arranged by size:
608 167 43 13
StuI AGG'CCT
Cuts at: 0 28 427 713 831
Size: 28 399 286 118
Fragments arranged by size:
399 286 118 28
StyI C'CwwG_G
Cuts at: 0 677 831
Size: 677 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 433 810 831
Size: 433 377 21
Fragments arranged by size:
433 377 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 652 684 831
Size: 652 32 147
Fragments arranged by size:
652 147 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 831
Size: 168 663
XmaI C'CCGG_G
Cuts at: 0 9 831
Size: 9 822
Enzymes that do cut:
AarI AccI AcuI AflII AleI AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BciVI Bme1580I BmrI BpmI
Bpu10I BpuEI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstYI BtgI ClaI EaeI
Eco57MI EcoO109I HaeII MscI MslI MspA1I NcoI NspI
PasI PfoI PstI PvuII SbfI SfcI SmaI SmlI
StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI