Restriction map for
pFN21AB8780
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MseI
MslI MunI NarI NcoI NdeI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 189 CGCG CG^CG
AspHI 235 GTGCTC G[AT]GC[AT]^C
AvaI 333 CCCGGG C^[CT]CG[GA]G
BbsI 772 GTCTTC GAAGAC(2/6)
BfaI 229 CTAG C^TAG
BglI 577 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 405 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 405 GCTCAGC GC^T[ACGT]AGC
BpuAI 772 GTCTTC GAAGAC(2/6)
BseRI 649 GAGGAG GAGGAG(10/8)
BsiHKAI 235 GTGCTC G[AT]GC[AT]^C
BsoBI 333 CCCGGG C^[CT]CG[GA]G
BsrBI 415 CCGCTC CCGCTC(-3/-3)
BsrFI 659 GCCGGC [GA]^CCGG[CT]
BstUI 189 CGCG CG^CG
BstXI 604 CCAGGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 405 GCTCAGC GC^T[ACGT]AGC
Cfr10I 659 GCCGGC [GA]^CCGG[CT]
DpnII 45 GATC ^GATC
EarI 762 CTCTTC CTCTTC(1/4)
HgaI 549 GCGTC GACGC(5/10)
Ksp632I 762 CTCTTC CTCTTC(1/4)
MaeI 229 CTAG C^TAG
MaeIII 66 GTCAC ^GT[ACGT]AC
MboI 45 GATC ^GATC
MvnI 189 CGCG CG^CG
NaeI 659 GCCGGC GCC^GGC
NdeII 45 GATC ^GATC
NgoMIV 659 GCCGGC G^CCGGC
NheI 228 GCTAGC G^CTAGC
PpuMI 692 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 45 GATC ^GATC
SmaI 333 CCCGGG CCC^GGG
StuI 653 AGGCCT AGG^CCT
TaqI 479 TCGA T^CGA
Tsp45I 66 GTCAC ^GT[GC]AC
TthHB8I 479 TCGA T^CGA
XcmI 710 CCAGCGACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 333 CCCGGG C^CCGGG
XmaI 333 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 375 GTAC GT^AC
739 GTAC
AlwNI 55 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
346 CAGTGTCTG
ApaI 204 GGGCCC GGGCC^C
753 GGGCCC
BalI 278 TGGCCA TGG^CCA
326 TGGCCA
BanII 204 GGGCCC G[GA]GC[CT]^C
753 GGGCCC
BsaI 389 GAGACC GGTCTC(1/5)
530 GAGACC
BspMI 377 ACCTGC ACCTGC(4/8)
735 GCAGGT
BstF5I 96 CATCC GGATG(2/0)
642 CATCC
CfoI 190 GCGC GCG^C
222 GCGC
DdeI 406 CTCAG C^T[ACGT]AG
742 CTCAG
EaeI 278 TGGCCA [CT]^GGCC[GA]
326 TGGCCA
EcoNI 645 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
720 CCTGGCCCAGG
FokI 96 CATCC GGATG(9/13)
642 CATCC
HhaI 190 GCGC GCG^C
222 GCGC
HinP1I 190 GCGC G^CGC
222 GCGC
HphI 484 GGTGA GGTGA(8/7)
194 TCACC
MboII 763 TCTTC GAAGA(8/7)
773 TCTTC
MluNI 278 TGGCCA TGG^CCA
326 TGGCCA
MscI 278 TGGCCA TGG^CCA
326 TGGCCA
RsaI 375 GTAC GT^AC
739 GTAC
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HinfI HpaII ItaI MnlI MspA1I
MspI MvaI MwoI NciI NlaIV
PleI PstI PvuII Sau96I ScrFI
SfcI TseI TspRI