(Circular) (Six-base) MAPSORT of: pFN21AB8780.seq Check: 6581 from: 1 to: 5482 pFN21AB8780 5482 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3292 278 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 1937 Size: 888 4594 Acc65I G'GTAC_C Cuts at: 2803 2803 Size: 5482 AclI AA'CG_TT Cuts at: 1563 3609 3982 1563 Size: 2046 373 3063 Fragments arranged by size: 3063 2046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2132 3545 4929 5197 1632 Size: 455 45 1413 1384 268 1917 Fragments arranged by size: 1917 1413 1384 455 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5285 AflIII A'CryG_T Cuts at: 1203 4381 5358 1203 Size: 3178 977 1327 Fragments arranged by size: 3178 1327 977 AgeI A'CCGG_T Cuts at: 4313 4313 Size: 5482 AhdI GACnn_n'nnGTC Cuts at: 4211 4211 Size: 5482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5482 AlwNI CAG_nnn'CTG Cuts at: 2065 2356 3225 4797 2065 Size: 291 869 1572 2750 Fragments arranged by size: 2750 1572 869 291 ApaI G_GGCC'C Cuts at: 2213 2762 2213 Size: 549 4933 ApaLI G'TGCA_C Cuts at: 3539 4695 3539 Size: 1156 4326 ApoI r'AATT_y Cuts at: 1493 2791 3017 3169 5278 1493 Size: 1298 226 152 2109 1697 Fragments arranged by size: 2109 1697 1298 226 152 AseI AT'TA_AT Cuts at: 160 1032 4036 160 Size: 872 3004 1606 Fragments arranged by size: 3004 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 AvaI C'yCGr_G Cuts at: 1870 1943 2338 2807 1870 Size: 73 395 469 4545 Fragments arranged by size: 4545 469 395 73 BamHI G'GATC_C Cuts at: 2812 3179 2812 Size: 367 5115 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2803 4258 618 Size: 325 217 627 1016 1455 1842 Fragments arranged by size: 1842 1455 1016 627 325 217 BanII G_rGCy'C Cuts at: 1391 2213 2762 2801 1391 Size: 822 549 39 4072 Fragments arranged by size: 4072 822 549 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2770 928 Size: 971 871 3640 Fragments arranged by size: 3640 971 871 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3673 3707 1903 Size: 34 1736 34 3678 Fragments arranged by size: 3678 1736 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3374 4584 3374 Size: 1210 4272 BclI T'GATC_A Cuts at: 1548 1548 Size: 5482 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2588 4093 136 Size: 107 122 71 631 605 916 1505 1525 Fragments arranged by size: 1525 1505 916 631 605 122 107 71 BglII A'GATC_T Cuts at: 5477 5477 Size: 5482 BlpI GC'TnA_GC Cuts at: 2411 2899 2411 Size: 488 4994 Bme1580I G_kGCm'C Cuts at: 1790 2213 2762 3543 4699 1790 Size: 423 549 781 1156 2573 Fragments arranged by size: 2573 1156 781 549 423 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5482 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2452 2626 3213 Size: 416 53 83 399 24 1035 174 587 Cuts at: 3213 4171 442 Size: 958 1753 Fragments arranged by size: 1753 1035 958 587 416 399 174 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2237 1056 Size: 1181 4301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1998 2114 2189 2294 2396 2548 2672 4142 Size: 759 116 75 105 102 152 124 1470 Cuts at: 4142 1239 Size: 2579 Fragments arranged by size: 2579 1470 759 152 124 116 105 102 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3607 4472 4770 3607 Size: 865 298 4319 Fragments arranged by size: 4319 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2388 2529 4145 882 Size: 617 889 141 1616 2219 Fragments arranged by size: 2219 1616 889 617 141 BsaAI yAC'GTr Cuts at: 493 1204 3273 493 Size: 711 2069 2702 Fragments arranged by size: 2702 2069 711 BsaBI GATnn'nnATC Cuts at: 5476 5476 Size: 5482 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3289 3671 4306 275 Size: 53 83 186 936 1756 382 635 1451 Fragments arranged by size: 1756 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3915 4313 4587 4734 5145 3915 Size: 398 274 147 411 4252 Fragments arranged by size: 4252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5257 Fragments arranged by size: 5257 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 1186 Size: 1483 3999 BseYI C'CCAG_C Cuts at: 2058 2360 2558 2592 2630 2694 4685 5181 2058 Size: 302 198 34 38 64 1991 496 2359 Fragments arranged by size: 2359 1991 496 302 198 64 38 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4909 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2932 3693 4721 1540 Size: 275 186 931 761 1028 2301 Fragments arranged by size: 2301 1028 931 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 3543 3628 4699 2244 Size: 1299 85 1071 3027 Fragments arranged by size: 3027 1299 1071 85 BsmI GAATG_Cn' Cuts at: 2993 3086 2993 Size: 93 5389 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2213 2244 2762 2801 3543 3628 4699 Size: 399 423 31 518 39 742 85 1071 Cuts at: 4699 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 518 423 399 85 39 31 BspEI T'CCGG_A Cuts at: 5145 5145 Size: 5482 BspHI T'CATG_A Cuts at: 3371 5101 3371 Size: 1730 3752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 2731 2837 844 Size: 1547 340 106 3489 Fragments arranged by size: 3489 1547 340 106 BsrBI CCG'CTC Cuts at: 1394 1823 2422 3369 1394 Size: 429 599 947 3507 Fragments arranged by size: 3507 947 599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3977 4151 65 Size: 1183 2729 174 1396 Fragments arranged by size: 2729 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2664 4126 4313 1700 Size: 110 854 1462 187 2869 Fragments arranged by size: 2869 1462 854 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5482 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5482 BssSI C'ACGA_G Cuts at: 3542 4554 3542 Size: 1012 4470 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5482 BstBI TT'CG_AA Cuts at: 4286 4286 Size: 5482 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5482 BstXI CCAn_nnnn'nTGG Cuts at: 2616 2616 Size: 5482 BstYI r'GATC_y Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477 Size: 840 367 385 17 1452 86 12 346 Cuts at: 5477 1972 Size: 1977 Fragments arranged by size: 1977 1452 840 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4743 Fragments arranged by size: 4743 683 56 BtsI GCAGTG_nn' Cuts at: 2357 2681 2998 3792 3812 2357 Size: 324 317 794 20 4027 Fragments arranged by size: 4027 794 324 317 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5447 DraI TTT'AAA Cuts at: 2786 2786 Size: 5482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4489 809 Size: 507 3173 1802 Fragments arranged by size: 3173 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 2331 2929 Size: 54 1425 50 275 18 453 48 598 Cuts at: 2929 3818 5447 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 598 453 275 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2929 1537 Size: 275 1117 4090 Fragments arranged by size: 4090 1117 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2773 3412 1353 Size: 1420 639 3423 Fragments arranged by size: 3423 1420 639 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2055 2750 4069 4443 4589 387 Size: 1064 138 466 695 1319 374 146 1280 Fragments arranged by size: 1319 1280 1064 695 466 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1998 2087 2114 2132 2189 2294 2396 Size: 393 366 89 27 18 57 105 102 Cuts at: 2396 2548 2672 3545 4142 4929 5197 1239 Size: 152 124 873 597 787 268 1524 Fragments arranged by size: 1524 873 787 597 393 366 268 152 124 105 102 89 57 27 18 EcoNI CCTnn'n_nnAGG Cuts at: 2654 2729 2654 Size: 75 5407 EcoO109I rG'GnC_Cy Cuts at: 2573 2698 2759 2573 Size: 125 61 5296 Fragments arranged by size: 5296 125 61 EcoRI G'AATT_C Cuts at: 2791 3169 2791 Size: 378 5104 FspI TGC'GCA Cuts at: 3988 3988 Size: 5482 HaeII r_GCGC'y Cuts at: 1678 4629 1678 Size: 2951 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 4123 4155 4197 Size: 32 135 32 462 32 2057 32 42 Cuts at: 4197 4229 1373 Size: 32 2626 Fragments arranged by size: 2626 2057 462 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2826 3072 669 Size: 988 281 888 246 3079 Fragments arranged by size: 3079 988 888 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5482 HpaI GTT'AAC Cuts at: 3072 3072 Size: 5482 KpnI G_GTAC'C Cuts at: 2807 2807 Size: 5482 MfeI C'AATT_G Cuts at: 3081 3081 Size: 5482 MluI A'CGCG_T Cuts at: 5358 5358 Size: 5482 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 4596 4780 1305 Size: 972 2319 184 2007 Fragments arranged by size: 2319 2007 972 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2285 2333 10 Size: 54 1425 343 453 48 3159 Fragments arranged by size: 3159 1425 453 343 54 48 MslI CAynn'nnrTG Cuts at: 518 1166 3440 3799 3958 518 Size: 648 2274 359 159 2042 Fragments arranged by size: 2274 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2062 2215 2347 2527 2898 3575 4723 Size: 412 44 153 132 180 371 677 1148 Cuts at: 4723 4968 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 412 371 245 180 153 132 44 NaeI GCC'GGC Cuts at: 2666 2666 Size: 5482 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5482 NgoMIV G'CCGG_C Cuts at: 2664 2664 Size: 5482 NheI G'CTAG_C Cuts at: 1052 2233 1052 Size: 1181 4301 NotI GC'GGCC_GC Cuts at: 2929 2929 Size: 5482 NspI r_CATG'y Cuts at: 1132 1229 2840 4385 1132 Size: 97 1611 1545 2229 Fragments arranged by size: 2229 1611 1545 97 PasI CC'CwG_GG Cuts at: 1348 2598 1348 Size: 1250 4232 PciI A'CATG_T Cuts at: 4381 4381 Size: 5482 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5482 PfoI T'CCnGG_A Cuts at: 2454 2649 2673 2454 Size: 195 24 5263 Fragments arranged by size: 5263 195 24 PmeI GTTT'AAAC Cuts at: 2786 2786 Size: 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3551 3583 5064 5096 3551 Size: 32 1481 32 3937 Fragments arranged by size: 3937 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2698 2698 Size: 5482 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5482 PsiI TTA'TAA Cuts at: 3052 3052 Size: 5482 PspOMI G'GGCC_C Cuts at: 2209 2758 2209 Size: 549 4933 PstI C_TGCA'G Cuts at: 830 2049 2352 2452 2688 2834 830 Size: 1219 303 100 236 146 3478 Fragments arranged by size: 3478 1219 303 236 146 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5482 PvuII CAG'CTG Cuts at: 2062 2347 2527 2062 Size: 285 180 5017 Fragments arranged by size: 5017 285 180 SalI G'TCGA_C Cuts at: 1936 2824 1936 Size: 888 4594 SbfI CC_TGCA'GG Cuts at: 2834 2834 Size: 5482 ScaI AGT'ACT Cuts at: 3730 5442 3730 Size: 1712 3770 SfcI C'TryA_G Cuts at: 826 1045 2045 2348 2448 2684 2830 3965 4646 Size: 219 1000 303 100 236 146 1135 681 Cuts at: 4646 4837 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1000 681 303 236 219 191 146 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5482 SmaI CCC'GGG Cuts at: 1872 2340 2809 1872 Size: 468 469 4545 Fragments arranged by size: 4545 469 468 SmlI C'TyrA_G Cuts at: 820 1017 1943 3586 4487 4749 820 Size: 197 926 1643 901 262 1553 Fragments arranged by size: 1643 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3273 493 Size: 2780 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5482 SphI G_CATG'C Cuts at: 1132 2840 1132 Size: 1708 3774 SspI AAT'ATT Cuts at: 5 52 3406 5 Size: 47 3354 2081 Fragments arranged by size: 3354 2081 47 StuI AGG'CCT Cuts at: 1517 2660 1517 Size: 1143 4339 StyI C'CwwG_G Cuts at: 513 1065 2004 2921 513 Size: 552 939 917 3074 Fragments arranged by size: 3074 939 917 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2216 2590 3509 3526 3679 3864 4309 Size: 1255 636 374 919 17 153 185 445 Cuts at: 4309 325 Size: 1498 Fragments arranged by size: 1498 1255 919 636 445 374 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3728 5440 96 Size: 274 80 33 51 1443 1751 1712 138 Fragments arranged by size: 1751 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5442 5474 5442 Size: 32 5450 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5482 XbaI T'CTAG_A Cuts at: 2818 2818 Size: 5482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2722 2722 Size: 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5482 XmaI C'CCGG_G Cuts at: 1870 2338 2807 1870 Size: 468 469 4545 Fragments arranged by size: 4545 469 468 XmnI GAAnn'nnTTC Cuts at: 3611 3611 Size: 5482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3290 276 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI