(Circular) (Six-base) MAPSORT of: pFN21AB8778.seq Check: 4677 from: 1 to: 5479 pFN21AB8778 5479 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2329 2329 Size: 5479 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3289 278 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2822 1937 Size: 885 4594 Acc65I G'GTAC_C Cuts at: 2800 2800 Size: 5479 AclI AA'CG_TT Cuts at: 1563 3606 3979 1563 Size: 2043 373 3063 Fragments arranged by size: 3063 2043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3542 4926 5194 1632 Size: 1910 1384 268 1917 Fragments arranged by size: 1917 1910 1384 268 AfeI AGC'GCT Cuts at: 2297 2297 Size: 5479 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5282 AflIII A'CryG_T Cuts at: 1203 2175 4378 5355 1203 Size: 972 2203 977 1327 Fragments arranged by size: 2203 1327 977 972 AgeI A'CCGG_T Cuts at: 4310 4310 Size: 5479 AhdI GACnn_n'nnGTC Cuts at: 4208 4208 Size: 5479 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5479 AlwNI CAG_nnn'CTG Cuts at: 2194 2344 2558 3222 4794 2194 Size: 150 214 664 1572 2879 Fragments arranged by size: 2879 1572 664 214 150 ApaI G_GGCC'C Cuts at: 2133 2515 2679 2133 Size: 382 164 4933 Fragments arranged by size: 4933 382 164 ApaLI G'TGCA_C Cuts at: 3536 4692 3536 Size: 1156 4323 ApoI r'AATT_y Cuts at: 1493 2788 3014 3166 5275 1493 Size: 1295 226 152 2109 1697 Fragments arranged by size: 2109 1697 1295 226 152 AscI GG'CGCG_CC Cuts at: 2261 2261 Size: 5479 AseI AT'TA_AT Cuts at: 160 1032 4033 160 Size: 872 3001 1606 Fragments arranged by size: 3001 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 AvaI C'yCGr_G Cuts at: 1870 1943 2126 2146 2165 2508 2532 2804 1870 Size: 73 183 20 19 343 24 272 4545 Fragments arranged by size: 4545 343 272 183 73 24 20 19 BamHI G'GATC_C Cuts at: 2809 3176 2809 Size: 367 5112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2429 2800 4255 618 Size: 325 217 627 642 371 1455 1842 Fragments arranged by size: 1842 1455 642 627 371 325 217 BanII G_rGCy'C Cuts at: 1391 2133 2252 2515 2679 2712 2798 1391 Size: 742 119 263 164 33 86 4072 Fragments arranged by size: 4072 742 263 164 119 86 33 BbeI G_GCGC'C Cuts at: 2433 2433 Size: 5479 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2618 928 Size: 971 719 3789 Fragments arranged by size: 3789 971 719 BbvCI CC'TCA_GC Cuts at: 2159 2159 Size: 5479 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3670 3704 1903 Size: 34 1733 34 3678 Fragments arranged by size: 3678 1733 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3371 4581 3371 Size: 1210 4269 BclI T'GATC_A Cuts at: 1548 1548 Size: 5479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 4090 136 Size: 107 122 71 631 605 538 1880 1525 Fragments arranged by size: 1880 1525 631 605 538 122 107 71 BglII A'GATC_T Cuts at: 5474 5474 Size: 5479 BlpI GC'TnA_GC Cuts at: 2896 2896 Size: 5479 Bme1580I G_kGCm'C Cuts at: 1790 2133 2451 2515 2679 3540 4696 1790 Size: 343 318 64 164 861 1156 2573 Fragments arranged by size: 2573 1156 861 343 318 164 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5479 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3210 4168 442 Size: 416 53 83 399 24 1793 958 1753 Fragments arranged by size: 1793 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2106 1056 Size: 1050 4429 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 4139 1239 Size: 1139 1761 2579 Fragments arranged by size: 2579 1761 1139 Bpu10I CC'TnA_GC Cuts at: 2159 2159 Size: 5479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3604 4469 4767 3604 Size: 865 298 4316 Fragments arranged by size: 4316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2711 4142 882 Size: 617 1212 1431 2219 Fragments arranged by size: 2219 1431 1212 617 BsaAI yAC'GTr Cuts at: 493 1204 3270 493 Size: 711 2066 2702 Fragments arranged by size: 2702 2066 711 BsaBI GATnn'nnATC Cuts at: 5473 5473 Size: 5479 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2430 3286 3668 4303 Size: 53 83 186 936 897 856 382 635 Cuts at: 4303 275 Size: 1451 Fragments arranged by size: 1451 936 897 856 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2290 3912 4310 4584 4731 5142 2290 Size: 1622 398 274 147 411 2627 Fragments arranged by size: 2627 1622 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2359 2389 2528 2554 2558 Size: 30 165 30 956 30 139 26 4 Cuts at: 2558 2584 1178 Size: 26 4073 Fragments arranged by size: 4073 956 165 139 30 30 30 26 26 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2001 2060 2134 2669 1186 Size: 815 59 74 535 3996 Fragments arranged by size: 3996 815 535 74 59 BseYI C'CCAG_C Cuts at: 2344 2551 2558 4682 5178 2344 Size: 207 7 2124 496 2645 Fragments arranged by size: 2645 2124 496 207 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2200 1163 Size: 573 464 4442 Fragments arranged by size: 4442 573 464 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3690 4718 1540 Size: 275 186 928 761 1028 2301 Fragments arranged by size: 2301 1028 928 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2712 3540 3625 4696 2712 Size: 828 85 1071 3495 Fragments arranged by size: 3495 1071 828 85 BsmI GAATG_Cn' Cuts at: 2990 3083 2990 Size: 93 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2252 2451 2515 2679 2712 2798 Size: 399 343 119 199 64 164 33 86 Cuts at: 2798 3540 3625 4696 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 199 164 119 86 85 64 33 BspEI T'CCGG_A Cuts at: 2290 5142 2290 Size: 2852 2627 BspHI T'CATG_A Cuts at: 3368 5098 3368 Size: 1730 3749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2329 2834 844 Size: 1485 505 3489 Fragments arranged by size: 3489 1485 505 BsrBI CCG'CTC Cuts at: 1394 1823 2315 2392 3366 1394 Size: 429 492 77 974 3507 Fragments arranged by size: 3507 974 492 429 77 BsrDI GCAATG_nn' Cuts at: 65 1248 3974 4148 65 Size: 1183 2726 174 1396 Fragments arranged by size: 2726 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2119 4123 4310 1700 Size: 110 309 2004 187 2869 Fragments arranged by size: 2869 2004 309 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5479 BssHII G'CGCG_C Cuts at: 1925 2261 1925 Size: 336 5143 BssSI C'ACGA_G Cuts at: 3539 4551 3539 Size: 1012 4467 BstAPI GCAn_nnn'nTGC Cuts at: 794 2194 794 Size: 1400 4079 BstBI TT'CG_AA Cuts at: 4283 4283 Size: 5479 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5479 BstYI r'GATC_y Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474 Size: 837 367 385 17 1452 86 12 346 Cuts at: 5474 1972 Size: 1977 Fragments arranged by size: 1977 1452 837 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2281 2425 2664 513 Size: 552 1216 144 239 3328 Fragments arranged by size: 3328 1216 552 239 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4740 Fragments arranged by size: 4740 683 56 BtsI GCAGTG_nn' Cuts at: 2995 3789 3809 2995 Size: 794 20 4665 Fragments arranged by size: 4665 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5444 DraI TTT'AAA Cuts at: 2783 2783 Size: 5479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4486 809 Size: 507 3170 1802 Fragments arranged by size: 3170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444 Size: 54 1425 50 275 18 1096 889 1629 Cuts at: 5444 8 Size: 43 Fragments arranged by size: 1629 1425 1096 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 1537 Size: 275 1114 4090 Fragments arranged by size: 4090 1114 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3409 1353 Size: 2056 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2129 2542 4066 4440 4586 387 Size: 1064 138 540 413 1524 374 146 1280 Fragments arranged by size: 1524 1280 1064 540 413 374 146 138 EcoICRI GAG'CTC Cuts at: 2710 2710 Size: 5479 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 3542 4139 4926 5194 1239 Size: 393 746 1164 597 787 268 1524 Fragments arranged by size: 1524 1164 787 746 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2605 2605 Size: 5479 EcoO109I rG'GnC_Cy Cuts at: 2370 2562 2370 Size: 192 5287 EcoRI G'AATT_C Cuts at: 2788 3166 2788 Size: 378 5101 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 5479 FspI TGC'GCA Cuts at: 2302 2357 3985 2302 Size: 55 1628 3796 Fragments arranged by size: 3796 1628 55 HaeII r_GCGC'y Cuts at: 1678 2237 2299 2433 4626 1678 Size: 559 62 134 2193 2531 Fragments arranged by size: 2531 2193 559 134 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2359 2391 2528 2554 2560 Size: 32 135 32 787 32 137 26 6 Cuts at: 2560 2586 4120 4152 4194 4226 1373 Size: 26 1534 32 42 32 2626 Fragments arranged by size: 2626 1534 787 137 135 42 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 2823 3069 669 Size: 988 281 885 246 3079 Fragments arranged by size: 3079 988 885 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5479 HpaI GTT'AAC Cuts at: 3069 3069 Size: 5479 KasI G'GCGC_C Cuts at: 2429 2429 Size: 5479 KpnI G_GTAC'C Cuts at: 2804 2804 Size: 5479 MfeI C'AATT_G Cuts at: 3078 3078 Size: 5479 MluI A'CGCG_T Cuts at: 2175 5355 2175 Size: 3180 2299 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4593 4777 1305 Size: 3288 184 2007 Fragments arranged by size: 3288 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3657 Fragments arranged by size: 3657 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3437 3796 3955 518 Size: 648 2271 359 159 2042 Fragments arranged by size: 2271 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2184 2219 2283 2558 2895 3572 4720 4965 Size: 578 35 64 275 337 677 1148 245 Cuts at: 4965 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 578 337 275 245 64 35 NarI GG'CG_CC Cuts at: 2430 2430 Size: 5479 NcoI C'CATG_G Cuts at: 513 1065 2664 513 Size: 552 1599 3328 Fragments arranged by size: 3328 1599 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5479 NheI G'CTAG_C Cuts at: 1052 2102 1052 Size: 1050 4429 NotI GC'GGCC_GC Cuts at: 2926 2926 Size: 5479 NspI r_CATG'y Cuts at: 1132 1229 2837 4382 1132 Size: 97 1608 1545 2229 Fragments arranged by size: 2229 1608 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5479 PciI A'CATG_T Cuts at: 4378 4378 Size: 5479 PflMI CCAn_nnn'nTGG Cuts at: 1108 2558 1108 Size: 1450 4029 PmeI GTTT'AAAC Cuts at: 2783 2783 Size: 5479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3548 3580 5061 5093 3548 Size: 32 1481 32 3934 Fragments arranged by size: 3934 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2562 2562 Size: 5479 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5479 PsiI TTA'TAA Cuts at: 3049 3049 Size: 5479 PspOMI G'GGCC_C Cuts at: 2129 2511 2675 2129 Size: 382 164 4933 Fragments arranged by size: 4933 382 164 PstI C_TGCA'G Cuts at: 830 2831 830 Size: 2001 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5479 PvuII CAG'CTG Cuts at: 2184 2184 Size: 5479 SacI G_AGCT'C Cuts at: 2712 2712 Size: 5479 SacII CC_GC'GG Cuts at: 2284 2284 Size: 5479 SalI G'TCGA_C Cuts at: 1936 2821 1936 Size: 885 4594 SanDI GG'GwC_CC Cuts at: 2562 2562 Size: 5479 SbfI CC_TGCA'GG Cuts at: 2831 2831 Size: 5479 ScaI AGT'ACT Cuts at: 3727 5439 3727 Size: 1712 3767 SfcI C'TryA_G Cuts at: 826 1045 2827 3962 4643 4834 826 Size: 219 1782 1135 681 191 1471 Fragments arranged by size: 1782 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2431 2431 Size: 5479 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5479 SmaI CCC'GGG Cuts at: 1872 2128 2510 2534 2806 1872 Size: 256 382 24 272 4545 Fragments arranged by size: 4545 382 272 256 24 SmlI C'TyrA_G Cuts at: 820 1017 1943 3583 4484 4746 820 Size: 197 926 1640 901 262 1553 Fragments arranged by size: 1640 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3270 493 Size: 2777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5479 SphI G_CATG'C Cuts at: 1132 2837 1132 Size: 1705 3774 SspI AAT'ATT Cuts at: 5 52 3403 5 Size: 47 3351 2081 Fragments arranged by size: 3351 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5479 StyI C'CwwG_G Cuts at: 513 1065 2640 2664 2918 513 Size: 552 1575 24 254 3074 Fragments arranged by size: 3074 1575 552 254 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3506 3523 3676 3861 4306 325 Size: 1255 1926 17 153 185 445 1498 Fragments arranged by size: 1926 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3725 5437 96 Size: 274 80 33 51 1443 1748 1712 138 Fragments arranged by size: 1748 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5439 5471 5439 Size: 32 5447 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5479 XbaI T'CTAG_A Cuts at: 2815 2815 Size: 5479 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5479 XmaI C'CCGG_G Cuts at: 1870 2126 2508 2532 2804 1870 Size: 256 382 24 272 4545 Fragments arranged by size: 4545 382 272 256 24 XmnI GAAnn'nnTTC Cuts at: 3608 3608 Size: 5479 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3287 276 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI XcmI