(Circular) (Six-base) MAPSORT of: pFN21AB8777.seq Check: 4199 from: 1 to: 5476 pFN21AB8777 5476 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2271 2271 Size: 5476 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3286 278 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2819 1937 Size: 882 4594 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 3603 3976 1563 Size: 2040 373 3063 Fragments arranged by size: 3063 2040 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 2231 2504 2744 3539 4923 5191 1632 Size: 521 78 273 240 795 1384 268 1917 Fragments arranged by size: 1917 1384 795 521 273 268 240 78 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 4375 5352 1203 Size: 3172 977 1327 Fragments arranged by size: 3172 1327 977 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 4205 4205 Size: 5476 AleI CACnn'nnGTG Cuts at: 1166 2528 2615 1166 Size: 1362 87 4027 Fragments arranged by size: 4027 1362 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5476 AlwNI CAG_nnn'CTG Cuts at: 2138 2263 2569 2589 3219 4791 2138 Size: 125 306 20 630 1572 2823 Fragments arranged by size: 2823 1572 630 306 125 20 ApaI G_GGCC'C Cuts at: 2775 2775 Size: 5476 ApaLI G'TGCA_C Cuts at: 3533 4689 3533 Size: 1156 4320 ApoI r'AATT_y Cuts at: 1493 2785 3011 3163 5272 1493 Size: 1292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 152 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2052 2180 2767 2801 1870 Size: 73 109 128 587 34 4545 Fragments arranged by size: 4545 587 128 109 73 34 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2414 2447 2414 Size: 33 5443 BamHI G'GATC_C Cuts at: 2385 2806 3173 2385 Size: 421 367 4688 Fragments arranged by size: 4688 421 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2093 2118 2579 2709 Size: 325 217 627 222 84 25 461 130 Cuts at: 2709 2797 4252 618 Size: 88 1455 1842 Fragments arranged by size: 1842 1455 627 461 325 222 217 130 88 84 25 BanII G_rGCy'C Cuts at: 1391 2775 2795 1391 Size: 1384 20 4072 Fragments arranged by size: 4072 1384 20 BbeI G_GCGC'C Cuts at: 2097 2097 Size: 5476 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2140 928 Size: 971 241 4264 Fragments arranged by size: 4264 971 241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 1903 Size: 34 1730 34 3678 Fragments arranged by size: 3678 1730 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2306 4087 136 Size: 107 122 71 631 605 634 1781 1525 Fragments arranged by size: 1781 1525 634 631 605 122 107 71 BglII A'GATC_T Cuts at: 2229 5471 2229 Size: 3242 2234 BlpI GC'TnA_GC Cuts at: 2893 2893 Size: 5476 Bme1580I G_kGCm'C Cuts at: 1790 2012 2123 2775 3537 4693 1790 Size: 222 111 652 762 1156 2573 Fragments arranged by size: 2573 1156 762 652 222 111 BmgBI CAC'GTC Cuts at: 1318 2617 1318 Size: 1299 4177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3207 4165 442 Size: 416 53 83 399 24 1790 958 1753 Fragments arranged by size: 1790 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 4136 1239 Size: 1496 1401 2579 Fragments arranged by size: 2579 1496 1401 Bpu10I CC'TnA_GC Cuts at: 2273 2273 Size: 5476 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3601 4466 4764 3601 Size: 865 298 4313 Fragments arranged by size: 4313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2050 4139 882 Size: 617 551 2089 2219 Fragments arranged by size: 2219 2089 617 551 BsaAI yAC'GTr Cuts at: 493 1204 3267 493 Size: 711 2063 2702 Fragments arranged by size: 2702 2063 711 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 2397 3283 3665 Size: 53 83 186 936 561 303 886 382 Cuts at: 3665 4300 275 Size: 635 1451 Fragments arranged by size: 1451 936 886 635 561 382 303 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 4307 4581 4728 5139 3909 Size: 398 274 147 411 4246 Fragments arranged by size: 4246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2216 2246 2459 2489 1178 Size: 30 165 30 813 30 213 30 4165 Fragments arranged by size: 4165 813 213 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2552 1186 Size: 877 489 4110 Fragments arranged by size: 4110 877 489 BseYI C'CCAG_C Cuts at: 2735 4679 5175 2735 Size: 1944 496 3036 Fragments arranged by size: 3036 1944 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 2249 2429 2552 1163 Size: 573 333 180 180 123 4087 Fragments arranged by size: 4087 573 333 180 180 123 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2926 3687 4715 1540 Size: 275 186 925 761 1028 2301 Fragments arranged by size: 2301 1028 925 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 3537 3622 4693 2208 Size: 1329 85 1071 2991 Fragments arranged by size: 2991 1329 1071 85 BsmI GAATG_Cn' Cuts at: 2987 3080 2987 Size: 93 5383 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2123 2208 2775 2795 3537 3622 Size: 399 222 111 85 567 20 742 85 Cuts at: 3622 4693 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 567 399 222 111 85 85 20 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 2831 844 Size: 1427 560 3489 Fragments arranged by size: 3489 1427 560 BsrBI CCG'CTC Cuts at: 1394 1823 3363 1394 Size: 429 1540 3507 Fragments arranged by size: 3507 1540 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3971 4145 65 Size: 1183 2723 174 1396 Fragments arranged by size: 2723 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2115 2268 4120 4307 1700 Size: 110 305 153 1852 187 2869 Fragments arranged by size: 2869 1852 305 187 153 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5476 BssSI C'ACGA_G Cuts at: 3536 4548 3536 Size: 1012 4464 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5476 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 2629 1184 Size: 1445 4031 BstXI CCAn_nnnn'nTGG Cuts at: 2617 2617 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2229 2284 2385 2806 3173 3558 3575 5027 Size: 257 55 101 421 367 385 17 1452 Cuts at: 5027 5113 5125 5471 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 421 385 367 346 257 101 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2652 513 Size: 552 939 648 3337 Fragments arranged by size: 3337 939 648 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2446 2992 3786 3806 2446 Size: 546 794 20 4116 Fragments arranged by size: 4116 794 546 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4483 809 Size: 507 3167 1802 Fragments arranged by size: 3167 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2337 2619 Size: 54 1425 50 275 18 363 144 282 Cuts at: 2619 2923 3812 5441 8 Size: 304 889 1629 43 Fragments arranged by size: 1629 1425 889 363 304 282 275 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2923 1537 Size: 275 1111 4090 Fragments arranged by size: 4090 1111 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2081 3406 1353 Size: 719 9 1325 3423 Fragments arranged by size: 3423 1325 719 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2308 4063 4437 4583 387 Size: 1064 138 719 1755 374 146 1280 Fragments arranged by size: 1755 1280 1064 719 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2231 2504 2735 2744 3539 4136 Size: 393 521 78 273 231 9 795 597 Cuts at: 4136 4923 5191 1239 Size: 787 268 1524 Fragments arranged by size: 1524 795 787 597 521 393 273 268 231 78 9 EcoNI CCTnn'n_nnAGG Cuts at: 2171 2465 2171 Size: 294 5182 EcoO109I rG'GnC_Cy Cuts at: 2164 2176 2250 2344 2470 2755 2771 2164 Size: 12 74 94 126 285 16 4869 Fragments arranged by size: 4869 285 126 94 74 16 12 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 2097 2418 2742 4623 1678 Size: 419 321 324 1881 2531 Fragments arranged by size: 2531 1881 419 324 321 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 4117 4149 4191 Size: 32 135 32 911 32 1602 32 42 Cuts at: 4191 4223 1373 Size: 32 2626 Fragments arranged by size: 2626 1602 911 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2820 3066 669 Size: 988 281 882 246 3079 Fragments arranged by size: 3079 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KasI G'GCGC_C Cuts at: 2093 2093 Size: 5476 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4590 4774 1305 Size: 3285 184 2007 Fragments arranged by size: 3285 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2621 10 Size: 54 1425 343 789 2865 Fragments arranged by size: 2865 1425 789 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 2615 3434 3793 3952 518 Size: 648 1362 87 819 359 159 2042 Fragments arranged by size: 2042 1362 819 648 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2892 3569 4717 4962 1606 Size: 1286 677 1148 245 2120 Fragments arranged by size: 2120 1286 1148 677 245 NarI GG'CG_CC Cuts at: 2094 2094 Size: 5476 NcoI C'CATG_G Cuts at: 513 1065 2004 2652 513 Size: 552 939 648 3337 Fragments arranged by size: 3337 939 648 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2834 4379 1132 Size: 97 1605 1545 2229 Fragments arranged by size: 2229 1605 1545 97 PasI CC'CwG_GG Cuts at: 1348 2401 1348 Size: 1053 4423 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5476 PfoI T'CCnGG_A Cuts at: 2241 2241 Size: 5476 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 2755 2176 Size: 579 4897 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PspOMI G'GGCC_C Cuts at: 2771 2771 Size: 5476 PstI C_TGCA'G Cuts at: 830 2156 2466 2594 2828 830 Size: 1326 310 128 234 3478 Fragments arranged by size: 3478 1326 310 234 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 SalI G'TCGA_C Cuts at: 1936 2818 1936 Size: 882 4594 SbfI CC_TGCA'GG Cuts at: 2466 2828 2466 Size: 362 5114 ScaI AGT'ACT Cuts at: 3724 5436 3724 Size: 1712 3764 SfcI C'TryA_G Cuts at: 826 1045 2152 2462 2590 2824 3959 4640 4831 Size: 219 1107 310 128 234 1135 681 191 Cuts at: 4831 826 Size: 1471 Fragments arranged by size: 1471 1135 1107 681 310 234 219 191 128 SfoI GGC'GCC Cuts at: 2095 2095 Size: 5476 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2054 2769 2803 1872 Size: 182 715 34 4545 Fragments arranged by size: 4545 715 182 34 SmlI C'TyrA_G Cuts at: 820 1017 1943 3580 4481 4743 820 Size: 197 926 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 3400 5 Size: 47 3348 2081 Fragments arranged by size: 3348 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5476 StyI C'CwwG_G Cuts at: 513 1065 2004 2391 2652 2751 2915 513 Size: 552 939 387 261 99 164 3074 Fragments arranged by size: 3074 939 552 387 261 164 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3503 3520 3673 3858 4303 325 Size: 1255 1923 17 153 185 445 1498 Fragments arranged by size: 1923 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 3722 5434 Size: 274 80 33 51 1443 696 1049 1712 Cuts at: 5434 96 Size: 138 Fragments arranged by size: 1712 1443 1049 696 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2459 2491 5436 5468 2459 Size: 32 2945 32 2467 Fragments arranged by size: 2945 2467 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5476 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2398 2398 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2052 2767 2801 1870 Size: 182 715 34 4545 Fragments arranged by size: 4545 715 182 34 XmnI GAAnn'nnTTC Cuts at: 3605 3605 Size: 5476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3284 276 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI