Restriction map for
pFN21AB8776
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BseAI BseRI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AgeI 1012 ACCGGT A^CCGGT
AlwI 1173 GATCC GGATC(4/5)
AlwNI 1398 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 1525 GGTCC G^G[AT]CC
BanI 1331 GGCACC G^G[CT][GA]CC
BbvI 1283 GCTGC GCAGC(8/12)
BcnI 1056 CCGGG CC^[GC]GG
BfaI 868 CTAG C^TAG
BlpI 812 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 812 GCTCAGC GC^T[ACGT]AGC
BsaI 1438 GAGACC GGTCTC(1/5)
BsgI 1118 GTGCAG GTGCAG(16/14)
BsmBI 242 CGTCTC CGTCTC(1/5)
BstYI 1172 AGATCC [GA]^GATC[CT]
CelII 812 GCTCAGC GC^T[ACGT]AGC
Cfr13I 1525 GGTCC G^G[ACGT]CC
DpnII 1173 GATC ^GATC
DraII 1524 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 645 CTCTTC CTCTTC(1/4)
EcoO109I 1524 GGGTCCC [GA]G^G[ACGT]CC[CT]
Fnu4HI 1283 GCTGC GC^[ACGT]GC
HaeIII 1321 GGCC GG^CC
HindIII 797 AAGCTT A^AGCTT
ItaI 1283 GCTGC GC^[ACGT]GC
Ksp632I 645 CTCTTC CTCTTC(1/4)
MaeI 868 CTAG C^TAG
MboI 1173 GATC ^GATC
MflI 1172 AGATCC [GA]^GATC[CT]
NciI 1056 CCGGG CC^[GC]GG
NdeII 1173 GATC ^GATC
PinAI 1012 ACCGGT A^CCGGT
PpuMI 1524 GGGTCCC [GA]G^G[AT]CC[CT]
Sau3AI 1173 GATC ^GATC
Sau96I 1525 GGTCC G^G[ACGT]CC
SgrAI 1011 CACCGGTG C[GA]^CCGG[CT]G
SmlI 138 CTTGAG C^T[CT][GA]AG
TseI 1283 GCTGC G^C[AT]GC
XhoII 1172 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 314 CCGC CCGC(-3/-1)
1127 GCGG
BanII 558 GAGCTC G[GA]GC[CT]^C
1566 GAGCTC
BsaWI 676 ACCGGA [AT]^CCGG[AT]
1012 ACCGGT
BsmI 778 GAATGC GAATGC(1/-1)
862 GAATGC
BsrFI 1012 ACCGGT [GA]^CCGG[CT]
1532 GCCGGT
BstF5I 899 CATCC GGATG(2/0)
1571 CATCC
Cac8I 223 GCAAGC GC[ACGT]^[ACGT]GC
1318 GCTGGC
Cfr10I 1012 ACCGGT [GA]^CCGG[CT]
1532 GCCGGT
DsaI 395 CCATGG C^C[GA][CT]GG
1613 CCATGG
EcoRI 836 GAATTC G^AATTC
1424 GAATTC
EcoT14I 395 CCATGG C^C[AT][AT]GG
1613 CCATGG
FauI 313 CCCGC CCCGC(4/6)
1127 GCGGG
FokI 899 CATCC GGATG(9/13)
1571 CATCC
MboII 413 GAAGA GAAGA(8/7)
646 TCTTC
NcoI 395 CCATGG C^CATGG
1613 CCATGG
PflMI 390 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1456 CCAGAATGTGG
SacI 558 GAGCTC GAGCT^C
1566 GAGCTC
StyI 395 CCATGG C^C[AT][AT]GG
1613 CCATGG
TaqI 1226 TCGA T^CGA
1581 TCGA
Tsp45I 308 GTGAC ^GT[GC]AC
359 GTGAC
TthHB8I 1226 TCGA T^CGA
1581 TCGA
Van91I 390 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1456 CCAGAATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AspHI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI DdeI EcoRII
HapII HinfI HpaII HphI MaeIII
MnlI MseI MslI MspI MvaI
NlaIII NlaIV PleI RsaI ScrFI
SfaNI TfiI Tru9I Tsp509I TspRI
XmnI