(Circular) (Six-base) MAPSORT of: pFN21AB8773.seq Check: 4371 from: 1 to: 5428 pFN21AB8773 5428 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2433 2433 Size: 5428 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2771 1937 Size: 834 4594 Acc65I G'GTAC_C Cuts at: 2749 2749 Size: 5428 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 3491 4875 5143 1632 Size: 410 1449 1384 268 1917 Fragments arranged by size: 1917 1449 1384 410 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 2121 4327 5304 1203 Size: 918 2206 977 1327 Fragments arranged by size: 2206 1327 977 918 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5428 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2528 2560 2528 Size: 32 5396 AlwNI CAG_nnn'CTG Cuts at: 2384 2439 3171 4743 2384 Size: 55 732 1572 3069 Fragments arranged by size: 3069 1572 732 55 ApaI G_GGCC'C Cuts at: 2621 2621 Size: 5428 ApaLI G'TGCA_C Cuts at: 3485 4641 3485 Size: 1156 4272 ApoI r'AATT_y Cuts at: 1493 2737 2963 3115 5224 1493 Size: 1244 226 152 2109 1697 Fragments arranged by size: 2109 1697 1244 226 152 AseI AT'TA_AT Cuts at: 160 1032 3982 160 Size: 872 2950 1606 Fragments arranged by size: 2950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2457 2633 2753 1870 Size: 73 514 176 120 4545 Fragments arranged by size: 4545 514 176 120 73 BamHI G'GATC_C Cuts at: 2758 3125 2758 Size: 367 5061 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2251 2272 2413 2421 2606 Size: 325 217 627 464 21 141 8 185 Cuts at: 2606 2749 4204 618 Size: 143 1455 1842 Fragments arranged by size: 1842 1455 627 464 325 217 185 143 141 21 8 BanII G_rGCy'C Cuts at: 1391 2065 2166 2621 2634 2747 1391 Size: 674 101 455 13 113 4072 Fragments arranged by size: 4072 674 455 113 101 13 BbeI G_GCGC'C Cuts at: 2276 2276 Size: 5428 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2055 2084 928 Size: 971 156 29 4272 Fragments arranged by size: 4272 971 156 29 BbvCI CC'TCA_GC Cuts at: 2404 2404 Size: 5428 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3619 3653 1903 Size: 34 1682 34 3678 Fragments arranged by size: 3678 1682 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4530 3320 Size: 1210 4218 BclI T'GATC_A Cuts at: 1548 2491 1548 Size: 943 4485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4039 136 Size: 107 122 71 631 605 2367 1525 Fragments arranged by size: 2367 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5423 5423 Size: 5428 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 2338 2621 3489 4645 1790 Size: 548 283 868 1156 2573 Fragments arranged by size: 2573 1156 868 548 283 BmgBI CAC'GTC Cuts at: 1318 2053 2238 2554 1318 Size: 735 185 316 4192 Fragments arranged by size: 4192 735 316 185 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2390 3159 4117 Size: 416 53 83 399 24 973 769 958 Cuts at: 4117 442 Size: 1753 Fragments arranged by size: 1753 973 958 769 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5428 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2563 2563 Size: 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 2653 4088 1239 Size: 1292 122 1435 2579 Fragments arranged by size: 2579 1435 1292 122 Bpu10I CC'TnA_GC Cuts at: 2404 2404 Size: 5428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3553 4418 4716 3553 Size: 865 298 4265 Fragments arranged by size: 4265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2296 2717 4091 882 Size: 617 797 421 1374 2219 Fragments arranged by size: 2219 1374 797 617 421 BsaAI yAC'GTr Cuts at: 493 1204 2704 3219 493 Size: 711 1500 515 2702 Fragments arranged by size: 2702 1500 711 515 BsaBI GATnn'nnATC Cuts at: 5422 5422 Size: 5428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2273 3235 3617 4252 Size: 53 83 186 936 740 962 382 635 Cuts at: 4252 275 Size: 1451 Fragments arranged by size: 1451 962 936 740 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3861 4259 4533 4680 5091 3861 Size: 398 274 147 411 4198 Fragments arranged by size: 4198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2291 2321 1178 Size: 30 165 30 888 30 4285 Fragments arranged by size: 4285 888 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 1186 Size: 1045 4383 BseYI C'CCAG_C Cuts at: 2525 4631 5127 2525 Size: 2106 496 2826 Fragments arranged by size: 2826 2106 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2334 1163 Size: 573 598 4257 Fragments arranged by size: 4257 598 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2284 2878 3639 4667 1540 Size: 275 186 283 594 761 1028 2301 Fragments arranged by size: 2301 1028 761 594 283 275 186 BsiHKAI G_wGCw'C Cuts at: 3489 3574 4645 3489 Size: 85 1071 4272 Fragments arranged by size: 4272 1071 85 BsmI GAATG_Cn' Cuts at: 2939 3032 2939 Size: 93 5335 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2065 2166 2338 2621 2634 2747 3489 Size: 399 275 101 172 283 13 113 742 Cuts at: 3489 3574 4645 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 275 172 113 101 85 13 BspEI T'CCGG_A Cuts at: 5091 5091 Size: 5428 BspHI T'CATG_A Cuts at: 3317 5047 3317 Size: 1730 3698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2433 2783 844 Size: 1589 350 3489 Fragments arranged by size: 3489 1589 350 BsrBI CCG'CTC Cuts at: 1394 1823 2178 2269 2278 3315 1394 Size: 429 355 91 9 1037 3507 Fragments arranged by size: 3507 1037 429 355 91 9 BsrDI GCAATG_nn' Cuts at: 65 1248 2187 3923 4097 65 Size: 1183 939 1736 174 1396 Fragments arranged by size: 1736 1396 1183 939 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2069 4072 4259 1700 Size: 110 259 2003 187 2869 Fragments arranged by size: 2869 2003 259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5428 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5428 BssSI C'ACGA_G Cuts at: 2013 2042 3488 4500 2013 Size: 29 1446 1012 2941 Fragments arranged by size: 2941 1446 1012 29 BstAPI GCAn_nnn'nTGC Cuts at: 794 2342 794 Size: 1548 3880 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstXI CCAn_nnnn'nTGG Cuts at: 2533 2533 Size: 5428 BstYI r'GATC_y Cuts at: 1972 2208 2301 2712 2758 3125 3510 3527 4979 Size: 236 93 411 46 367 385 17 1452 Cuts at: 4979 5065 5077 5423 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 411 385 367 346 236 93 86 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 2295 513 Size: 552 1230 3646 Fragments arranged by size: 3646 1230 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4689 Fragments arranged by size: 4689 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3738 3758 2944 Size: 794 20 4614 Fragments arranged by size: 4614 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5393 DraI TTT'AAA Cuts at: 2732 2732 Size: 5428 DraIII CAC_nnn'GTG Cuts at: 2707 2707 Size: 5428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2288 4435 809 Size: 507 972 2147 1802 Fragments arranged by size: 2147 1802 972 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2067 2362 Size: 54 1425 50 275 18 208 29 295 Cuts at: 2362 2649 2875 3764 5393 8 Size: 287 226 889 1629 43 Fragments arranged by size: 1629 1425 889 295 287 275 226 208 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2875 1537 Size: 275 1063 4090 Fragments arranged by size: 4090 1063 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2532 3358 1353 Size: 665 514 826 3423 Fragments arranged by size: 3423 826 665 514 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4015 4389 4535 387 Size: 1064 138 2426 374 146 1280 Fragments arranged by size: 2426 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2531 2653 3491 4088 4875 5143 Size: 393 410 489 122 838 597 787 268 Cuts at: 5143 1239 Size: 1524 Fragments arranged by size: 1524 838 787 597 489 410 393 268 122 EcoO109I rG'GnC_Cy Cuts at: 2618 2660 2618 Size: 42 5386 EcoRI G'AATT_C Cuts at: 2737 3115 2737 Size: 378 5050 FspI TGC'GCA Cuts at: 3934 3934 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 2276 2439 4575 1678 Size: 598 163 2136 2531 Fragments arranged by size: 2531 2136 598 163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4069 4101 4143 4175 1373 Size: 32 135 32 2497 32 42 32 2626 Fragments arranged by size: 2626 2497 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2772 3018 669 Size: 988 281 834 246 3079 Fragments arranged by size: 3079 988 834 281 246 HindIII A'AGCT_T Cuts at: 748 2473 748 Size: 1725 3703 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KasI G'GCGC_C Cuts at: 2272 2272 Size: 5428 KpnI G_GTAC'C Cuts at: 2753 2753 Size: 5428 MfeI C'AATT_G Cuts at: 3027 3027 Size: 5428 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2706 4542 4726 1305 Size: 1401 1836 184 2007 Fragments arranged by size: 2007 1836 1401 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2040 10 Size: 54 1425 343 208 3398 Fragments arranged by size: 3398 1425 343 208 54 MslI CAynn'nnrTG Cuts at: 518 1166 2156 3386 3745 3904 518 Size: 648 990 1230 359 159 2042 Fragments arranged by size: 2042 1230 990 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2104 2529 2673 2844 3521 4669 4914 1606 Size: 498 425 144 171 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 498 425 245 171 144 NaeI GCC'GGC Cuts at: 2071 2071 Size: 5428 NarI GG'CG_CC Cuts at: 2273 2273 Size: 5428 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4876 NdeI CA'TA_TG Cuts at: 387 387 Size: 5428 NgoMIV G'CCGG_C Cuts at: 2069 2069 Size: 5428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5428 NotI GC'GGCC_GC Cuts at: 2875 2875 Size: 5428 NspI r_CATG'y Cuts at: 1132 1229 2125 2155 2786 4331 1132 Size: 97 896 30 631 1545 2229 Fragments arranged by size: 2229 1545 896 631 97 30 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5428 PciI A'CATG_T Cuts at: 2121 4327 2121 Size: 2206 3222 PflMI CCAn_nnn'nTGG Cuts at: 1108 2707 1108 Size: 1599 3829 PfoI T'CCnGG_A Cuts at: 2115 2115 Size: 5428 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PmlI CAC'GTG Cuts at: 2704 2704 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PspOMI G'GGCC_C Cuts at: 2617 2617 Size: 5428 PspXI vC'TCGA_Gb Cuts at: 2633 2633 Size: 5428 PstI C_TGCA'G Cuts at: 830 2103 2266 2430 2780 830 Size: 1273 163 164 350 3478 Fragments arranged by size: 3478 1273 350 164 163 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5428 PvuII CAG'CTG Cuts at: 2529 2673 2529 Size: 144 5284 RsrII CG'GwC_CG Cuts at: 2200 2200 Size: 5428 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SapI GCTCTTCn'nnn_ Cuts at: 2532 2532 Size: 5428 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 2586 3676 5388 2586 Size: 1090 1712 2626 Fragments arranged by size: 2626 1712 1090 SfcI C'TryA_G Cuts at: 826 1045 2099 2262 2426 2776 3911 4592 4783 Size: 219 1054 163 164 350 1135 681 191 Cuts at: 4783 826 Size: 1471 Fragments arranged by size: 1471 1135 1054 681 350 219 191 164 163 SfoI GGC'GCC Cuts at: 2274 2274 Size: 5428 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2755 1872 Size: 883 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2457 2633 3532 4433 4695 820 Size: 197 926 514 176 899 901 262 1553 Fragments arranged by size: 1553 926 901 899 514 262 197 176 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2786 1132 Size: 1654 3774 SspI AAT'ATT Cuts at: 5 52 3352 5 Size: 47 3300 2081 Fragments arranged by size: 3300 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5428 StyI C'CwwG_G Cuts at: 513 1065 2417 2867 513 Size: 552 1352 450 3074 Fragments arranged by size: 3074 1352 552 450 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3455 3472 3625 3810 4255 325 Size: 1255 1875 17 153 185 445 1498 Fragments arranged by size: 1875 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2584 3674 5386 Size: 274 80 33 51 1443 607 1090 1712 Cuts at: 5386 96 Size: 138 Fragments arranged by size: 1712 1443 1090 607 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 2291 2323 2359 2391 5388 5420 2222 Size: 32 37 32 36 32 2997 32 2230 Fragments arranged by size: 2997 2230 37 36 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2083 1316 Size: 767 4661 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 2457 2633 1943 Size: 514 176 4738 Fragments arranged by size: 4738 514 176 XmaI C'CCGG_G Cuts at: 1870 2753 1870 Size: 883 4545 XmnI GAAnn'nnTTC Cuts at: 3557 3557 Size: 5428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PsrI SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI